IDENTIFICATION OF HOST ORIGIN OF PARASITES OF BARK BEETLES (COLEOPTERA: SCOLYTIDAE) BY FATTY ACID COMPOSITION

1981 ◽  
Vol 113 (3) ◽  
pp. 205-212 ◽  
Author(s):  
Louis H. Kudon ◽  
C. Wayne Berisford

AbstractThe free fatty acid composition of the southern pine beetle, Dendroctonus frontalis Zimmermann, and associated bark beetles was determined by gas-liquid chromatography. The lipid composition of several species of hymenopterous parasites matched the bark beetle hosts from which they were reared. Lipids from field collected parasites were compared with the lipid composition of possible bark beetle hosts to determine host of origin. Parasites ovipositing on a host were usually found to have a lipid composition matching that host. Approximately 20% of the parasites that were observed attempting to parasitize the southern pine beetle apparently developed on other bark beetle hosts.

1993 ◽  
Vol 56 (4) ◽  
pp. 302-305 ◽  
Author(s):  
V. K. JUNEJA ◽  
P. M. DAVIDSON

The sensitivity of Listeria monocytogenes Scott A and ATCC 19114 to antimicrobial compounds was altered when bacterial membrane lipid composition was modified by growth in the presence of added fatty acids. Analysis of cellular fatty acid composition by gas-liquid chromatography indicated that L. monocytogenes Scott A cells contained 0.97, 2.32, 0.81, and 0.72% (relative) of C14:0, C16:0, C18:0, and C18:l, respectively. In the presence of exogenously supplied C14:0, C16:0, C18:0, and C18:l, the percentages increased to 14.03, 30.92, 16.30, and 27.90%. Average MICs for L. monocytogenes Scott A and ATCC 19114 to sodium chloride, tertiary butylhydroquinone, methyl paraben, and propyl paraben were 10.0%, 81, 1406, and 544 μg/ml, respectively. Growing either strain in the presence of 50 μg/ml of either exogenously added C14:0 or C18:0 fatty acids increased their resistance to the four antimicrobial compounds. However, growth in the presence of C18:1 led to increased sensitivity to the antimicrobial agents. The results indicate that the susceptibility of L. monocytogenes to antimicrobial agents is related to the lipid composition of the cell membrane. Consequently, food preservation processes which alter fatty acid composition of L. monocytogenes could result in changes in antimicrobial susceptibility.


2020 ◽  
Author(s):  
Stephen R Clarke ◽  
Jessica Hartshorn

Abstract The southern pine beetle (SPB) Dendroctonus frontalis Zimmermann, is the most important insect pest of pines in the southeastern United States, with outbreaks often resulting in thousands of hectares of pine mortality. Natural enemies and competitors have been cited as significant regulators of SPB populations and, therefore, outbreaks. A recent outbreak on the Homochitto National Forest (NF) in Mississippi provided an opportunity to undertake a case study comparing population fluctuations of SPB, its major predator Thanasimus dubius, and its competitors, Ips bark beetles. Trap catches of all three were tracked through the course of the outbreak on the Homochitto NF as well as in two other forests with low or no SPB activity. The number of predators collected initially increased on the Homochitto NF in response to the SPB outbreak, but their impact on reducing infestation numbers was unclear. Numbers of Ips trapped were similar across all three forests, indicating that other factors were regulating SPB populations. The outbreak only lasted a single year, and its brevity likely limited the availability of host resources for natural enemy and competitor populations. Additional studies are warranted to explore the mechanisms affecting the extent and duration of SPB outbreaks, such as active forest management. Study Implications: The widespread application of cut-and-leave treatments during a short duration southern pine beetle (SPB) outbreak on the Homochitto National Forest did not result in immediate increased collections of Ips bark beetles, competitors of SPB. Similar population trends of Ips bark beetles in areas with and without SPB infestations suggest that other factors, such as climate, regulate population numbers. Low numbers of the main SPB predator, Thanasimus dubius, immediately preceded an outbreak, and trap collections increased after outbreak onset, reaffirming the importance of this natural enemy in SPB population fluctuations.


PEDIATRICS ◽  
1989 ◽  
Vol 83 (4) ◽  
pp. 632-632
Author(s):  
JOEL BITMAN ◽  
MARGIT HAMOSH ◽  
D. L. WOOD ◽  
L. M. FREED ◽  
P. HAMOSH

This report from Golembeski and Emery adds to the small quantity of literature in this field. Drs Golembeski and Emery claim that they are presenting information that counters our statement,"The abnormalities in fatty acid composition of the cystic fibrosis milk may contraindicate its use for the nursing infant." However, no data regarding fatty acid composition were presented. In their Table, only total lipids are shown. In our study, we acknowledged that mean total lipids were sufficient to supply the energy needs of the nursing infant.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 232-233
Author(s):  
Ekaterina Karpova ◽  
Ludmila Chizhova ◽  
Eugenia Surzhikova

Abstract The early period of postnatal ontogeny of sheep is characterized by a variety of metabolic processes that result in the formation and consolidation of a certain type of metabolism. A special role is played by lipids, which together with proteins and carbohydrates constitute the bulk of organic compounds, cells and the organism as a whole. The purpose of this research was to study the polymorphism of CAST and GH genes as well as its association with the fatty acid composition of blood plasma lipids in lambs. The content of fatty acids was determined by gas-liquid chromatography in the form of methyl esters. From the data obtained, it follows that in all studied ontogeny periods the peripheral blood of sheep with CASTNN and CASTMM genotypes had a higher concentration of fatty acids such as palmitic, stearic, linoleic, and arachidonic acids. The content is as follows: 27.58, 45.40, 3.16, 0.28% vs. 26.21, 45.31, 3.62, 0.18% at the age of 2 months; 25.07, 43.25, 6.05, 1.74% vs. 23.24, 38.76, 6.49, 1.32% at the age of 4 months; 20.64, 22.26, 14.17, 3.38% vs. 17.64, 19.91, 15.07, 2.46% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Studies of blood lipid composition of GHBBand GHAA genotype carriers showed that palmitic, stearic, linoleic, and arachidonic acids were dominant: 27.95, 27.96, 19.04% vs. 26.08, 23.88, 17.56% at the age of 2 months, 46.87, 39.87, 22.37% vs. 3.24, 6.87, 15.56% at the age of 4 months; 0.45, 1.68, 3.81% vs. 0.29, 1.29, 3.19% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Thus, this study emphasizes that there is a close relationship between the ratio of fatty acids in total blood lipids in lambs and the intensity of growth and development, ensuring and controlling the direction of biochemical processes in the growth and development of young sheep.


2021 ◽  
pp. 56-59
Author(s):  
Лев Арсенович Оганесянц ◽  
Александр Львович Панасюк ◽  
Елена Ивановна Кузьмина ◽  
Дмитрий Александрович Свиридов ◽  
Михаил Юрьевич Ганин

Продукты масложировой промышленности играют важную роль в формировании рациона питания человека. При этом особое внимание отводится растительным маслам ввиду их высокой физиологической ценности. На сегодняшний день достаточно остро стоит вопрос о способах их идентификации. В связи с тем, что стоимость растительного масла в значительной степени обуславливается выбором сырья, наиболее распространенным видом фальсификации является внесение недобросовестными производителями в готовую продукцию более дешевых видов масел. Приведен анализ современных способов идентификации растительных масел из различного сырья. До настоящего времени метод газожидкостной хроматографии являлся основным при выявлении примесей посторонних жиров в продукции по ее жирнокислотному составу. Также для выявления фальсификатов могут быть использованы такие инструментальные методы, как ЯМР-спектроскопия, ИК-спектроскопия, а также метод изотопной масс-спектрометрии, который является наиболее перспективным. Особый научный интерес представляет идентификация растительных масел по их географическому месту происхождения. Основываясь на анализе литературы, показано, что наибольшее распространение при установлении региональной принадлежности масел получили исследования, направленные на изучение изотопных характеристик углерода (13С/12С), кислорода (18O/16O) и водорода (2H/1H) элементов, входящих в состав продукта, а также его жирнокислотного состава. Описаны преимущества комплексного подхода исследований, включающего в себя создание массива данных, состоящего из значений различных показателей, и его глубокий анализ с использованием статистических методов анализа. Математическая модель может быть усилена данными элементного профиля масла, изотопных характеристик отдельно взятых жирных кислот или содержанием фенольных соединений. Products of the fat and oil industry play an important role in shaping human diet. At the same time, special attention is paid to vegetable oils in view of their high physiological value. Today, the question of how to identify them is quite acute. Due to the fact that the cost of vegetable oil is largely determined by the choice of raw materials, the most common falsification type is the introduction of cheaper oils types into finished products by unscrupulous manufacturers. The authors provide an analysis of modern methods for identifying vegetable oils from various raw materials. It has been shown that the gas-liquid chromatography method is the main one in identifying products by their fatty acid composition and makes it possible to establish the presence of foreign fats impurities. Also, methods of isotope mass spectrometry, NMR spectroscopy, IR spectroscopy and electrophysical methods of analysis can be used to detect counterfeits. Of particular scientific interest is the vegetable oils identification by their geographical place of origin, which is very important for some countries of the European Union. Based on the analysis of literature, it has been shown that the most widespread in the regional oils affiliation establishing are researches aimed at researching the isotopic characteristics of carbon (13C/12C), oxygen (18O/16O) and hydrogen (2H/1H) of the elements that make up the product, and also its fatty acid composition. The advantages of an integrated research approach are described, including the creation of a data array consisting of the values of various indicators and its in-depth analysis using statistical analysis methods. The mathematical model can be strengthened by data on the elemental profile of the oil, isotopic characteristics of individual fatty acids, or the content of phenolic compounds.


1970 ◽  
Vol 42 (4) ◽  
pp. 455-464 ◽  
Author(s):  
Md Moshfekus Saleh-E-In ◽  
Sudhangshu Kumar Roy

Anethum sowa L. (Dill) seeds were investigated to determine the fatty acid composition and proximate analyses. The seeds contain 9.36 % fatty oil. The saturated and unsaturated fatty acids contributed 6.22% and 93.78% respectively of the oil. The per cent composition of the extracted oil was identified by Gas Liquid Chromatography (GLC). Among the six fatty acids identified from this study oleic acid contributed the highest proportion (87.10%), where as, linolenic, palmitic, stearic, behenic and arachidic all together contributed the rest (12.90%). Proximate analyses showed that A. sowa. seeds are good source of dietary fibre. Overall Dill seeds oil can be considered as a good source of oleic acid. Key words: Anethum sowa, dill seed oil, fatty acid composition, oleic acid, linolenic acid, Gas liquid chromatography. Bangladesh J. Sci. Ind. Res. 42(4), 455-464, 2007


1976 ◽  
Vol 86 (1) ◽  
pp. 151-154 ◽  
Author(s):  
P. Gregory ◽  
C. O. Grogan

SUMMARYGas liquid chromatography was used to investigate the influence of several T, S and unclassified cytoplasms on the fatty acid composition of oil from A632 and CrS4HLA maize seeds. The fatty acid compositions of the A632 and CrS4HLA seeds differed markedly from each other with respect to oleic and linoleic acids but were stable within each seed line with respect to all sixteen of the cytoplasms tested.


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