APPLICATION OF NUCLEAR POLYHEDROSIS VIRUS TO CONTROL BRUCE SPANWORM (LEPIDOPTERA: GEOMETRIDAE)

1980 ◽  
Vol 112 (7) ◽  
pp. 741-744 ◽  
Author(s):  
W. G. H. Ives ◽  
J. C. Cunningham
Keyword(s):  
British Columbia ◽  
Nuclear Polyhedrosis Virus ◽  
Detailed Examination ◽  
Maritime Provinces ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Bruce Spanworm

A polyhedrosis virus was found in collections of Bruce spanworm, Operophtera bruceata (Hulst), larvae from two locations in British Columbia in 1957 (O. N. Morris, pers. comm.). Bruce spanworm was prevalent in the Maritime Provinces and Quebec in 1963 and 1964 and a polyhedrosis virus was credited with terminating this outbreak (Forbes et al. 1964, 1965; Martineau 1964, 1965). A detailed examination of the virus from Quebec showed that it was a singly-embedded (unicapsid) nuclear polyhedrosis virus (Smirnoff 1964) which is classified as Baculovirus subgroup A (Matthews 1979).

AERIAL APPLICATION OF TWO BACULOVIRUSES AGAINST THE WESTERN SPRUCE BUDWORM, CHORISTONEURA OCCIDENTALIS FREEMAN (LEPIDOPTERA: TORTRICIDAE), IN BRITISH COLUMBIA

1989 ◽  
Vol 121 (3) ◽  
pp. 209-217 ◽  
Author(s):  
I.S. Otvos ◽  
J.C. Cunningham ◽  
W.J. Kaupp
Keyword(s):  
British Columbia ◽  
Nuclear Polyhedrosis Virus ◽  
Adult Emergence ◽  
Spruce Budworm ◽  
Choristoneura Occidentalis ◽  
Western Spruce Budworm ◽  
Granulosis Virus ◽  
Population Reduction ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

AbstractTwo viruses, one a nuclear polyhedrosis virus and the other a granulosis virus, were applied in an attempt to initiate epizootics in populations of western spruce budworm, Choristoneura occidentalis Freeman, on Douglas-fir trees, Pseudotsuga menziesii (Mirb.) Franco, in southeastern British Columbia. Two 172-ha plots were aerially treated in 1982 with 9.0 kg of lyophilized, virus-infected larval powder that was formulated in an emulsifiable oil tank mix and applied at 9.4 L per hectare. Each plot was treated when larval populations were at the peak of the fourth instar. The nuclear polyhedrosis virus was applied at 5.4 × 1011 polyhedral inclusion bodies per hectare and the granulosis virus at 1.7 × 1014 capsules per hectare. Results showed that the granulosis virus treatment caused 34.6% population reduction (Abbott’s formula) and the nuclear polyhedrosis virus 51.8%. Larvae from treated and check plots were reared individually in the laboratory and the incidence of viruses, parasitoids, and successful adult emergence was recorded. Studies m these plots continued in 1983 and 1984. Although vertical transmission of both viruses was evident, their impact on budworm mortality was less than in 1982. Consequently, the epizootics were not sufficiently intense to control the target insect population.

scholarly journals Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 704
Author(s):  
Qian Yu ◽  
Pengfei Chang ◽  
Xiaoxuan Liu ◽  
Peng Lü ◽  
Qi Tang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bombyx Mori ◽  
Nuclear Polyhedrosis Virus ◽  
Expression System ◽  
Efficient Production ◽  
Human Bocavirus ◽  
Adeno Associated Virus ◽  
Silkworm Pupae ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

Recombinant adeno-associated virus (AAV) vectors have broad application prospects in the field of gene therapy. The establishment of low-cost and large-scale manufacturing is now the general agenda for industry. The baculovirus-insect cell/larva expression system has great potential for these applications due to its scalability and predictable biosafety. To establish a more efficient production system, Bombyx mori pupae were used as a new platform and infected with recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV). The production of a chimeric recombinant adeno-associated virus (rAAV) serotype 2/human bocavirus type-1 (HBoV1) vector was used to evaluate the efficiency of this new baculovirus expression vector (BEV)–insect expression system. For this purpose, we constructed two recombinant BmNPVs, which were named rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP. The yields of rAAV2/HBoV1 derived from the rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP co-infected BmN cells exceeded 2 × 104 vector genomes (VG) per cell. The rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP can express stably for at least five passages. Significantly, rAAV2/HBoV1 could be efficiently generated from BmNPV-infected silkworm larvae and pupae at average yields of 2.52 × 1012 VG/larva and 4.6 × 1012 VG/pupa, respectively. However, the vectors produced from the larvae and pupae had a high percentage of empty particles, which suggests that further optimization is required for this platform in the future. Our work shows that silkworm pupae, as an efficient bioreactor, have great potential for application in the production of gene therapy vectors.

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