Membrane Rafts: Part 2: Looking for Functional Rafts in Cell Membranes (41:55)

SciVee ◽  
2009 ◽  
Keyword(s):  
Cell Membranes ◽  
Membrane Rafts

Use of Detergents to Study Membrane Rafts: The Good, the Bad, and the Ugly

2003 ◽  
Vol 384 (9) ◽  
pp. 1259-1263 ◽  
Author(s):  
H. Shogomori ◽  
D.A. Brown
Keyword(s):  
Lipid Rafts ◽  
Cell Membranes ◽  
Eukaryotic Cell ◽  
Membrane Rafts ◽  
Ionic Detergents ◽  
Wide Range ◽  
Range Of Functions ◽  
Acyl Chains

Abstract Eukaryotic cell membranes contain microdomains called lipid rafts, which are cholesterol-rich domains in which lipid acyl chains are tightly packed and highly extended. A variety of proteins associate preferentially with rafts, and this raft association is important in a wide range of functions. A powerful and widely used method for studying lipid rafts takes advantage of their insolubility in non-ionic detergents. Here we describe the basis of detergent insolubility, and review strengths, limitations, and unresolved puzzles regarding this method.

scholarly journals On scattered waves and lipid domains: detecting membrane rafts with X-rays and neutrons

Soft Matter ◽  
2015 ◽  
Vol 11 (47) ◽  
pp. 9055-9072 ◽  
Author(s):  
Drew Marquardt ◽  
Frederick A. Heberle ◽  
Jonathan D. Nickels ◽  
Georg Pabst ◽  
John Katsaras
Keyword(s):  
Cell Membranes ◽  
Model Membrane ◽  
Biological Role ◽  
Membrane Rafts ◽  
Lipid Domains ◽  
X Rays ◽  
Membrane Systems ◽  
Mesoscopic Structure

In order to understand the biological role of lipids in cell membranes, it is necessary to determine the mesoscopic structure of well-defined model membrane systems.

Segregation of Gangliosides GM1 and GD3 on Cell Membranes, Isolated Membrane Rafts, and Defined Supported Lipid Monolayers

2001 ◽  
Vol 382 (2) ◽  
Author(s):  
Kavita A. Vyas ◽  
Himatkumar V. Patel ◽  
Alka A. Vyas ◽  
Ronald L. Schnaar
Keyword(s):  
Cell Membranes ◽  
Membrane Rafts ◽  
Lipid Monolayers

Photoreceptor disk membrane substructures by means of the complementary freeze-fracture-replica methods

1978 ◽  
Vol 36 (2) ◽  
pp. 156-157
Author(s):  
A. Tonosaki ◽  
M. Yamasaki ◽  
H. Washioka ◽  
J. Mizoguchi
Keyword(s):  
Cell Membranes ◽  
Freeze Fracture ◽  
Purple Membrane ◽  
Remarkable Case ◽  
Single Face ◽  
Disk Membrane ◽  
Associated Proteins ◽  
Photoreceptor Membranes

A vertebrate disk membrane is composed of 40 % lipids and 60 % proteins. Its fracture faces have been classed into the plasmic (PF) and exoplasmic faces (EF), complementary with each other, like those of most other types of cell membranes. The hypothesis assuming the PF particles as representing membrane-associated proteins has been challenged by serious questions if they in fact emerge from the crystalline formation or decoration effects during freezing and shadowing processes. This problem seems to be yet unanswered, despite the remarkable case of the purple membrane of Halobacterium, partly because most observations have been made on the replicas from a single face of specimen, and partly because, in the case of photoreceptor membranes, the conformation of a rhodopsin and its relatives remains yet uncertain. The former defect seems to be partially fulfilled with complementary replica methods.

Introduction to Cell Membranes at Molecular Resolution

1978 ◽  
Vol 36 (3) ◽  
pp. 497-502
Author(s):  
R.J. Barrnett
Keyword(s):  
Cell Membranes ◽  
International Federation ◽  
Mountain Range ◽  
Cellular Membranes ◽  
Short History ◽  
Biological Organization ◽  
Macromolecular Assemblies ◽  
The Past

This subject, is like observing the panorama of a mountain range, magnificent towering peaks, but it doesn't take much duration of observation to recognize that they are still in the process of formation. The mountains consist of approaches, materials and methods and the rocky substance of information has accumulated to such a degree that I find myself concentrating on the foothills in the foreground in order to keep up with the advance; the edifices behind form a wonderous, substantive background. It's a short history for such an accumulation and much of it has been moved by the members of the societies that make up this International Federation. My panel of speakers are here to provide what we hope is an interesting scientific fare, based on the fact that there is a continuum of biological organization from biochemical molecules through macromolecular assemblies and cellular membranes to the cell itself. Indeed, this fact explains the whole range of towering peaks that have emerged progressively during the past 25 years.

Membrane–cytoskeleton interactions in cholesterol-dependent domain formation

2015 ◽  
Vol 57 ◽  
pp. 177-187 ◽  
Author(s):  
Jennifer N. Byrum ◽  
William Rodgers
Keyword(s):  
Biological Properties ◽  
Membrane Rafts ◽  
Membrane Domains ◽  
Biological Functions ◽  
Membrane Cytoskeleton ◽  
Heterogeneous Structures ◽  
Integral Role ◽  
Model Cell ◽  
Actomyosin Cytoskeleton ◽  
Dependent Domain

Since the inception of the fluid mosaic model, cell membranes have come to be recognized as heterogeneous structures composed of discrete protein and lipid domains of various dimensions and biological functions. The structural and biological properties of membrane domains are represented by CDM (cholesterol-dependent membrane) domains, frequently referred to as membrane ‘rafts’. Biological functions attributed to CDMs include signal transduction. In T-cells, CDMs function in the regulation of the Src family kinase Lck (p56lck) by sequestering Lck from its activator CD45. Despite evidence of discrete CDM domains with specific functions, the mechanism by which they form and are maintained within a fluid and dynamic lipid bilayer is not completely understood. In the present chapter, we discuss recent advances showing that the actomyosin cytoskeleton has an integral role in the formation of CDM domains. Using Lck as a model, we also discuss recent findings regarding cytoskeleton-dependent CDM domain functions in protein regulation.

664: CXCL12/CXCR4 Trans-Activation of HER2 in Lipid Rafts of Prostate Cancer Cell Membranes Promotes Bone Metastasis

2007 ◽  
Vol 177 (4S) ◽  
pp. 223-223
Author(s):  
Sreenivasa R. Chinni ◽  
Hamilto Yamamoto ◽  
Zhong Dong ◽  
Aaron Sabbota ◽  
Sanaa Nabha ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Lipid Rafts ◽  
Cancer Cell ◽  
Cell Membranes ◽  
Prostate Cancer Cell
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