scholarly journals Low-molecular-weight Collagen Peptide Ameliorates Osteoarthritis Progression through Promoting Extracellular Matrix Synthesis by Chondrocytes in a Rabbit Anterior Cruciate Ligament Transection Model

2021 ◽  
Author(s):  
Mun-Hoe Lee ◽  
Hyeong-Min Kim ◽  
Hee-Chul Chung ◽  
Do-Un Kim ◽  
Jin-Hee Lee
Keyword(s):  
Molecular Weight ◽  
Extracellular Matrix ◽  
Anterior Cruciate Ligament ◽  
Cruciate Ligament ◽  
Low Molecular Weight ◽  
Anterior Cruciate Ligament Transection ◽  
Matrix Synthesis ◽  
Collagen Peptide ◽  
Osteoarthritis Progression ◽  
Anterior Cruciate

Influence of age on osteoarthritis progression after anterior cruciate ligament transection in rats

2014 ◽  
Vol 55 ◽  
pp. 44-48 ◽  
Author(s):  
María Luisa Ferrándiz ◽  
María Carmen Terencio ◽  
Ramón Ruhí ◽  
Josep Vergés ◽  
Eulàlia Montell ◽  
...  
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cruciate Ligament ◽  
Anterior Cruciate Ligament Transection ◽  
Osteoarthritis Progression ◽  
Anterior Cruciate

scholarly journals Comparison of pain behaviour and osteoarthritis progression between anterior cruciate ligament transection and osteochondral injury in rat models

2018 ◽  
Vol 7 (3) ◽  
pp. 244-251 ◽  
Author(s):  
T. Tawonsawatruk ◽  
O. Sriwatananukulkit ◽  
W. Himakhun ◽  
W. Hemstapat
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cruciate Ligament ◽  
Histological Evaluation ◽  
Anterior Cruciate Ligament Transection ◽  
Pain Behaviour ◽  
Rat Models ◽  
Injury Model ◽  
Osteochondral Injury ◽  
Osteoarthritis Progression ◽  
Anterior Cruciate

Objectives In this study, we compared the pain behaviour and osteoarthritis (OA) progression between anterior cruciate ligament transection (ACLT) and osteochondral injury in surgically-induced OA rat models. Methods OA was induced in the knee joints of male Wistar rats using transection of the ACL or induction of osteochondral injury. Changes in the percentage of high limb weight distribution (%HLWD) on the operated hind limb were used to determine the pain behaviour in these models. The development of OA was assessed and compared using a histological evaluation based on the Osteoarthritis Research Society International (OARSI) cartilage OA histopathology score. Results Both models showed an increase in joint pain as indicated by a significant (p < 0.05) decrease in the values of %HLWD at one week post-surgery. In the osteochondral injury model, the %HLWD returned to normal within three weeks, while in the ACLT model, a significant decrease in the %HLWD was persistent over an eight-week period. In addition, OA progression was more advanced in the ACLT model than in the osteochondral injury model. Furthermore, the ACLT model exhibited a higher mean OA score than that of the osteochondral injury model at 12 weeks. Conclusion The development of pain patterns in the ACLT and osteochondral injury models is different in that the OA progression was significant in the ACLT model. Although both can be used as models for a post-traumatic injury of the knee, the selection of appropriate models for OA in preclinical studies should be specified and relevant to the clinical scenario. Cite this article: T. Tawonsawatruk, O. Sriwatananukulkit, W. Himakhun, W. Hemstapat. Comparison of pain behaviour and osteoarthritis progression between anterior cruciate ligament transection and osteochondral injury in rat models. Bone Joint Res 2018;7:244–251. DOI: 10.1302/2046-3758.73.BJR-2017-0121.R2.

Anterior Cruciate Ligament Transection–Induced Cellular and Extracellular Events in Menisci: Implications for Osteoarthritis

2018 ◽  
Vol 46 (5) ◽  
pp. 1185-1198 ◽  
Author(s):  
Jing Xie ◽  
Demao Zhang ◽  
Yunfeng Lin ◽  
Quan Yuan ◽  
Xuedong Zhou
Keyword(s):  
Extracellular Matrix ◽  
Anterior Cruciate Ligament ◽  
Articular Cartilage ◽  
Lysyl Oxidase ◽  
Cruciate Ligament ◽  
Cartilage Destruction ◽  
Knee Joints ◽  
Inflammatory Factors ◽  
Anterior Cruciate Ligament Transection ◽  
Anterior Cruciate

Background: The meniscus plays an important role in knee joint diseases such as osteoarthritis (OA). Meniscal injuries can be accompanied by joint catabolic events initiated by inflammation, leading to articular cartilage destruction, but the cellular events responsible for intrinsic meniscal injury and the extracellular matrix changes necessary for meniscal degradation are not well known. Purpose: To explore the cellular and matrix-related changes of menisci based on a mouse OA model of anterior cruciate ligament transection (ACLT). Study Design: Controlled laboratory study. Methods: A mouse ACLT OA model was established by transection of anterior cruciate ligaments on the right knee joints of 8-week-old male (n = 34) and female (n = 34) C57 mice. The knee joints were collected at 1, 2, 4, and 8 weeks after ACLT surgery, and the meniscal changes were analyzed by radiography, histology, immunohistochemistry, immunoblot, and quantitative real-time polymerase chain reaction. Results: The deterioration of menisci was more extensive than that of articular cartilage and subchondral bone at 4 weeks after ACLT surgery. The rapid loss of collagen II and Sox9 in chondrocyte-like cells in the white-white zone of menisci was confirmed, and the activation of potential meniscus progenitor cells and chondroblasts was identified based on the increase of CD90, CD105, and Runx2. Further, the intrinsic inflammation in the bone marrow–like zone of menisci was activated by enhancement of dendritic cells (CD11c+), T cells (CD3+), and macrophages (F4/80+) with the increase of the inflammatory factors interleukin 1β and tumor necrosis factor α. Finally, the extracellular matrix events involving changes in chemokines, increases of matrix proteases (matrix metalloproteinases and ADAMTS5), and decreases of lysyl oxidase family were elucidated. Conclusion: ACLT-induced meniscal changes not only could explain the contribution of the meniscus to the progress of OA but also could provide a cue for initiation of preventive treatments in the early stages of OA. Clinical Relevance: This study provides support for better protection of menisci in ACL injury–induced conditions such as OA and indicates that menisci should be considered in the development of clinical pharmacological interventions.

scholarly journals High-resolution MRI and micro-CT in an ex vivo rabbit anterior cruciate ligament transection model of osteoarthritis

2004 ◽  
Vol 12 (8) ◽  
pp. 614-626 ◽  
Author(s):  
Danika L. Batiste ◽  
Alexandra Kirkley ◽  
Sheila Laverty ◽  
Lisa M.F. Thain ◽  
Alison R. Spouge ◽  
...  
Keyword(s):  
Anterior Cruciate Ligament ◽  
High Resolution ◽  
Ex Vivo ◽  
Cruciate Ligament ◽  
Micro Ct ◽  
Anterior Cruciate Ligament Transection ◽  
High Resolution Mri ◽  
Anterior Cruciate
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