scholarly journals Antimicrobial Peptides from Lactobacillus plantarum UTNGt2 Prevent Harmful Bacteria Growth on Fresh Tomatoes

2019 ◽  
Vol 29 (10) ◽  
pp. 1553-1560 ◽  
Author(s):  
Gabriela Nicoleta Tenea ◽  
Tatiana Delgado Pozo
Keyword(s):  
Antimicrobial Peptides ◽  
Lactobacillus Plantarum ◽  
Bacteria Growth

Lactobacillus plantarum ZJ316 improves the quality of Stachys sieboldii Miq. pickle by inhibiting harmful bacteria growth and degrading nitrite, promoting the gut microbiota health in vitro

2022 ◽  
Author(s):  
Shu Ting Hang ◽  
Ling zhou Zeng ◽  
Jia run Han ◽  
Zhong qin Zhang ◽  
Qingqing Zhou ◽  
...  
Keyword(s):  
Quality Control ◽  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Microbial Contamination ◽  
Nitrite Accumulation ◽  
Bacteria Growth ◽  
Lactobacillus Plantarum Zj316 ◽  
Stachys Sieboldii

Microbial contamination and nitrite accumulation are two major concerns on the quality control of fermented vegetables. In the present study, a lactic acid bacteria strain Lactobacillus plantarum ZJ316 (ZJ316) was...

scholarly journals Karakteristik Bakteri di Perairan Mangrove Pesisir Kraton Pasuruan (Characterization of Bacteria Isolated from Mangrove Coastal Waters of Kraton, Pasuruan)

2014 ◽  
Vol 19 (1) ◽  
pp. 35 ◽  
Author(s):  
Yahya Yahya ◽  
Happy Nursyam ◽  
Yenny Risjani ◽  
Seomarno Soemarno
Keyword(s):  
Pseudomonas Putida ◽  
Lactobacillus Plantarum ◽  
Bacillus Megaterium ◽  
Micrococcus Luteus ◽  
Avicennia Marina ◽  
Bacteria Growth ◽  
Rhizophora Apiculata ◽  
Sonneratia Alba ◽  
Avicennia Alba

Bakteri memainkan peran penting dalam ekosistem mangrove terutama dalam mengurai searasah daun. Keberadaan bakteri ini sangat dipengaruhi oleh berbagai faktor lingkungan. Penelitian ini bertujuan untuk mengisolasi dan karakterisasi bakteri dari perairan mangrove dari dengan vegetasi yang berbeda yakni Avicennia marina, Rhizophora apiculata, Avicennia alba dan Sonneratia alba. Pengambilan sampel dilakukan di perairan mangrove desa Bulu Kerto Kraton Pasuruan. Proses karakterisasi bakteri dilakukan di laboratorium mikrobiologi Fakultas Kedokteran Universitas Brawijaya Malang. Metode penelitian yang digunakan adalah eksploratif sedangkan parameter uji adalah isolasi bakteri dan uji morfologi, biokimia, serta deteksi pertumbuhan bakteri yang dominan. Hasil penelitian diperoleh 7 isolat bakteri Avicennia marina yaitu Nitrococcus sp., B. subtilis, B. pumilus, Pseudomonas putida. P.stutzeri, Micrococcus luteus, Vibrio sp, 6 isolat bakteri Rhizophora apiculata yaitu B. megaterium, Nitrococcus sp, Staphylococcus sp., P.putida, Lactobacillus sp., B. Subtilis,  8 isolat bakteri Avicennia alba yaitu B.megaterium, B.pumilus, B. subtilis, Nitrococcus sp., Pseudomonas putida, P. stutzeri, B. mycoides, Micrococcus sp.. dan 6 isolat bakteri di Sonneratia alba yaitu Bacillus megaterium, Nitrococcus sp., B.subtilis, Planococcus citreus, B. mycoides, Lactobacillus plantarum. Hasil ini menunjukkan bahwa jenis bakteri dipengaruhi oleh vegetasi mangrove yang ada di suatu perairan. Kata kunci: bakteri, perairan mangrove, vegetasi Bacteria play an important role in the ecosystem, especially in degrading mangrove leaves (litter). The presence of these bacteria is greatly influenced by various environmental factors. This research aims to isolate and characterized bacteria from mangrove waters with different vegetaion namely Avicennia marina, Rhizophora apiculata, Avicennia alba and Sonneratia alba. Characterization of the bacteria were done at microbiological laboratory in Medical Faculty, Brawijaya University. Several tests were done in this explorative research i.e. morphological test, biochemistry, and bacteria growth. The results showed there were 7 isolated bacterias found at Avicennia marina area i.e. Nitrococcus sp, B. subtilis, B. pumilus, Pseudomonas putida. P. stutzeri, Micrococcus luteus, Vibrio sp.; 6 isolated bacterias found at  Rhizophora apiculata area i.e. B. megaterium, Nitrococcus sp, Staphylococcus sp., P. putida, Lactobacillus sp., B. subtilis; 8  isolaed bacterias from Avicennia alba area i.e. B. megaterium, B. pumilus, B. subtilis, Nitrococcus sp., Pseudomonas putida, P. stutzeri, B. mycoides, Micrococcus sp., and 6 isolated bacterias from Sonneratia alba area i.e. Bacillus megaterium, Nitrococcus sp, B. subtilis, Planococcus citreus, B. mycoides, Lactobacillus plantarum. These results indicate that the type of bacteria is influenced by the existing mangrove vegetation. Keywords: bacteria, mangrove waters,vegetation

Effect of Isothiocyanates on the Activity of Lactobacillus plantarum Exposed to Irradiation

2020 ◽  
Vol 850 ◽  
pp. 219-224
Author(s):  
Kristīne Kalneniece ◽  
Toms Kusiņš ◽  
Inga Balode ◽  
Līva Mazkalniņa ◽  
Kārlis Švirksts ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Physiological Activity ◽  
Living Cells ◽  
Quinone Reductase ◽  
Tryptone Soya Broth ◽  
Bacteria Growth ◽  
Bacterial Cultures ◽  
Maximum Inhibition ◽  
De Man ◽  
Grown Culture

Two isothiocyanates, i.e., sulforaphane (SFA) and sulforaphene (SFE), are suggested to be used as an alternative chemopreventive diet. This study was focused on the effect of SFA and SFE on Lactobacillus plantarum, which has been subjected to the irradiation (2-50 Gy). The cultures grown in De Man, Rogosa and Sharpe (MRS) and Tryptone Soya Broth (TSB) were compared in terms of bacteria physiological activity under tested conditions. Broth composition notably influenced the bacteria growth kinetic parameters, as well as culture response to the oxidative stress. Activity of L. plantarum cells after irradiation was evaluated by their dehydrogenase (DHA) and quinone-reductase (QR) activities. The enzyme activity was quantified in living cells. Bacterial cultures obtained in MRS and TSB broth, demonstrated contrasting characteristics in their enzyme activities. The MRS-grown culture did not show any QR activity, whereas the TSB-grown cells showed a non-linear response towards gamma-irradiation with a maximum inhibition being at 10 Gy. Addition of SFA or SFE in concentration of 1 µg/mL to the cultures before irradiation exposure recovered the QR activity from 23% in a non-amended variant up to 102% and 121%, respectively, taking the non-irradiated non-amended variant as 100%.

scholarly journals Inoculum effect of antimicrobial peptides

2020 ◽  
Author(s):  
M. R. Loffredo ◽  
F. Savini ◽  
S. Bobone ◽  
B. Casciaro ◽  
H. Franzyk ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Cell Density ◽  
Bacterial Load ◽  
Initial Density ◽  
Resistant Bacteria ◽  
Bacterial Killing ◽  
Initial Inoculum ◽  
Bacteria Growth ◽  
Bacterial Drug Resistance ◽  
Inoculum Effect

AbstractThe activity of many antibiotics depends on the initial density of cells used in bacteria growth inhibition assays. This phenomenon, termed the inoculum effect, can have important consequences for the therapeutic efficacy of the drugs, since bacterial loads vary by several orders of magnitude in clinically relevant infections. Antimicrobial peptides are a promising class of molecules to fight drug-resistant bacteria, since they act mainly by perturbing the cell membranes rather than by inhibiting intracellular targets. Here we report the first systematic characterization of the inoculum effect for this class of antibacterial compounds. Thirteen peptides (including all-D enantiomers) and peptidomimetics were analyzed by measuring minimum inhibitory concentration values, covering more than 7 orders of magnitude in inoculated cell density. In all cases, we observed a significant inoculum effect for cell densities above 5 × 104 cells/mL, while the active concentrations remained constant (within the micromolar range) for lower densities. In the case of membrane-active peptides, these data can be rationalized by considering a simple model, taking into account peptide-cell association and hypothesizing that a threshold number of cell-bound peptide molecules is required in order to cause a killing effect. The observed effects question the clinical utility of activity and selectivity determinations performed at a fixed, standardized cell density. A routine evaluation of the inoculum dependence of the activity of antimicrobial peptides and peptidomimetics should be considered.Significance statementBacterial drug resistance is a crucial threat to global health and antimicrobials with novel mechanisms of action are severely needed. Antimicrobial peptides are natural molecules that kill bacteria mostly by perturbing their membranes and represent promising compounds to fight resistant microbes. Their activity is normally tested under standardized conditions of bacterial density. However, the bacterial load in clinically relevant infections varies by many orders of magnitude. Here we showed that the minimum peptide concentration needed for bacterial killing can vary by more than 100 times with an increase in the density of cells in the initial inoculum of the assay (inoculum effect) These findings question utility of the presently used activity screening assays and our current understanding of antimicrobial peptides.

scholarly journals Polydiacetylene Vesicles Acting as Colorimetric Sensor for the Detection of Plantaricin LD1 Purified From Lactobacillus Plantarum LD1

2021 ◽  
Author(s):  
Manoj Kumar Yadav ◽  
Santosh Kumar Tiwari
Keyword(s):  
Antimicrobial Peptides ◽  
Lactobacillus Plantarum ◽  
Pore Formation ◽  
Membrane Lipids ◽  
Membrane Disruption ◽  
Target Cells ◽  
Artificial Membrane ◽  
Physiological Processes ◽  
Colorimetric Response ◽  
Pda Vesicles

Abstract The interaction of antimicrobial peptides with membrane lipids plays a major role in numerous physiological processes. Bacteriocins are antimicrobial peptides known to kill target cells by pore formation and membrane disruption. In this study, polydiacetylene (PDA) vesicles were applied as artificial membrane for detection of plantaricin LD1 purified from Lactobacillus plantarum LD1. Plantaricin LD1 (200 µg/ml) was able to change the color of PDA vesicles from blue to red with colorimetric response CR % 30.26 ± 0.59. Nisin (200 µg/ml), used as control, also changed the color of the vesicles with CR % 50.56 ± 0.98 validating the membrane-acting nature of these bacteriocins. The PDA vesicles treated with nisin and plantaricin LD1 showed increased infrared absorbance at 1411.46 cm-1 and 1000-1150 cm-1 indicated the interaction of bacteriocins with phospholipids and fatty acids, respectively. Further, microscopic examination also suggested the disruption of bacteriocin-treated vesicles indicating the interaction of bacteriocins. These findings suggest that the PDA vesicles may be used as bio-mimetic sensor for the detection of bacteriocins produced by several probiotics in food and therapeutic applications.

scholarly journals Purification and Molecular Characterization of Two-Antimicrobial Peptides Produced by Lactobacillus plantarum DU10

2015 ◽  
Vol 9 (2) ◽  
pp. 46-58
Author(s):  
Zergui Amina ◽  
Saidi Noureddine ◽  
Arul Venkatesan ◽  
Bessaiah Hicham ◽  
Benmalek Yamina ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Molecular Characterization ◽  
Lactobacillus Plantarum
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