Antifungal effect of antimicrobial peptides (AMPs LR14) derived from Lactobacillus plantarum strain LR/14 and their applications in prevention of grain spoilage

2014 ◽  
Vol 42 ◽  
pp. 1-7 ◽  
Author(s):  
Ruchi Gupta ◽  
Sheela Srivastava
Keyword(s):  
Antimicrobial Peptides ◽  
Lactobacillus Plantarum ◽  
Antifungal Effect ◽  
Grain Spoilage

scholarly journals Polydiacetylene Vesicles Acting as Colorimetric Sensor for the Detection of Plantaricin LD1 Purified From Lactobacillus Plantarum LD1

2021 ◽  
Author(s):  
Manoj Kumar Yadav ◽  
Santosh Kumar Tiwari
Keyword(s):  
Antimicrobial Peptides ◽  
Lactobacillus Plantarum ◽  
Pore Formation ◽  
Membrane Lipids ◽  
Membrane Disruption ◽  
Target Cells ◽  
Artificial Membrane ◽  
Physiological Processes ◽  
Colorimetric Response ◽  
Pda Vesicles

Abstract The interaction of antimicrobial peptides with membrane lipids plays a major role in numerous physiological processes. Bacteriocins are antimicrobial peptides known to kill target cells by pore formation and membrane disruption. In this study, polydiacetylene (PDA) vesicles were applied as artificial membrane for detection of plantaricin LD1 purified from Lactobacillus plantarum LD1. Plantaricin LD1 (200 µg/ml) was able to change the color of PDA vesicles from blue to red with colorimetric response CR % 30.26 ± 0.59. Nisin (200 µg/ml), used as control, also changed the color of the vesicles with CR % 50.56 ± 0.98 validating the membrane-acting nature of these bacteriocins. The PDA vesicles treated with nisin and plantaricin LD1 showed increased infrared absorbance at 1411.46 cm-1 and 1000-1150 cm-1 indicated the interaction of bacteriocins with phospholipids and fatty acids, respectively. Further, microscopic examination also suggested the disruption of bacteriocin-treated vesicles indicating the interaction of bacteriocins. These findings suggest that the PDA vesicles may be used as bio-mimetic sensor for the detection of bacteriocins produced by several probiotics in food and therapeutic applications.

scholarly journals Purification and Molecular Characterization of Two-Antimicrobial Peptides Produced by Lactobacillus plantarum DU10

2015 ◽  
Vol 9 (2) ◽  
pp. 46-58
Author(s):  
Zergui Amina ◽  
Saidi Noureddine ◽  
Arul Venkatesan ◽  
Bessaiah Hicham ◽  
Benmalek Yamina ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Molecular Characterization ◽  
Lactobacillus Plantarum

Antimicrobial peptides in COPD

Pneumologie ◽  
2009 ◽  
Vol 63 (S 01) ◽  
Author(s):  
G Günther ◽  
E Andresen ◽  
J Bullwinkel ◽  
C Lange ◽  
H Heine
Keyword(s):  
Antimicrobial Peptides
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