Enterococcus faecium LKE12 Cell-Free Extract Accelerates Host Plant Growth via Gibberellin and Indole-3-Acetic Acid Secretion

2015 ◽  
Vol 25 (9) ◽  
pp. 1467-1475 ◽  
Author(s):  
Ko-Eun Lee ◽  
Ramalingam Radhakrishnan ◽  
Sang-Mo Kang ◽  
Young-Hyun You ◽  
Gil-Jae Joo ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Acid Secretion ◽  
Host Plant ◽  
Enterococcus Faecium ◽  
Cell Free Extract ◽  
Indole 3 Acetic Acid

scholarly journals Biochemical and Genetic Bases of Indole-3-Acetic Acid (Auxin Phytohormone) Degradation by the Plant-Growth-Promoting Rhizobacterium Paraburkholderia phytofirmans PsJN

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Raúl Donoso ◽  
Pablo Leiva-Novoa ◽  
Ana Zúñiga ◽  
Tania Timmermann ◽  
Gonzalo Recabarren-Gajardo ◽  
...  
Keyword(s):  
Energy Source ◽  
Gene Regulation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Plant Hormone ◽  
Content Type ◽  
Gene Functions ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

ABSTRACT Several bacteria use the plant hormone indole-3-acetic acid (IAA) as a sole carbon and energy source. A cluster of genes (named iac) encoding IAA degradation has been reported in Pseudomonas putida 1290, but the functions of these genes are not completely understood. The plant-growth-promoting rhizobacterium Paraburkholderia phytofirmans PsJN harbors iac gene homologues in its genome, but with a different gene organization and context than those of P. putida 1290. The iac gene functions enable P. phytofirmans to use IAA as a sole carbon and energy source. Employing a heterologous expression system approach, P. phytofirmans iac genes with previously undescribed functions were associated with specific biochemical steps. In addition, two uncharacterized genes, previously unreported in P. putida and found to be related to major facilitator and tautomerase superfamilies, are involved in removal of an IAA metabolite called dioxindole-3-acetate. Similar to the case in strain 1290, IAA degradation proceeds through catechol as intermediate, which is subsequently degraded by ortho-ring cleavage. A putative two-component regulatory system and a LysR-type regulator, which apparently respond to IAA and dioxindole-3-acetate, respectively, are involved in iac gene regulation in P. phytofirmans. These results provide new insights about unknown gene functions and complex regulatory mechanisms in IAA bacterial catabolism. IMPORTANCE This study describes indole-3-acetic acid (auxin phytohormone) degradation in the well-known betaproteobacterium P. phytofirmans PsJN and comprises a complete description of genes, some of them with previously unreported functions, and the general basis of their gene regulation. This work contributes to the understanding of how beneficial bacteria interact with plants, helping them to grow and/or to resist environmental stresses, through a complex set of molecular signals, in this case through degradation of a highly relevant plant hormone.

Rhizobium leguminosarum as a plant growth-promoting rhizobacterium: direct growth promotion of canola and lettuce

1996 ◽  
Vol 42 (3) ◽  
pp. 279-283 ◽  
Author(s):  
T. C. Noel ◽  
C. Sheng ◽  
C. K. Yost ◽  
R. P. Pharis ◽  
M. F. Hynes
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Rhizobium Leguminosarum ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Direct Growth ◽  
Early Seedling ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Early seedling root growth of the nonlegumes canola (Brassica campestris cv. Tobin, Brassica napus cv. Westar) and lettuce (Lactuca saliva cv. Grand Rapids) was significantly promoted by inoculation of seeds with certain strains of Rhizobium leguminosarum, including nitrogen- and nonnitrogen-fixing derivatives under gnotobiotic conditions. The growfh-promotive effect appears to be direct, with possible involvement of the plant growth regulators indole-3-acetic acid and cytokinin. Auxotrophic Rhizobium mutants requiring tryptophan or adenosine (precursors for indole-3-acetic acid and cytokinin synthesis, respectively) did not promote growth to the extent of the parent strain. The findings of this study demonstrate a new facet of the Rhizobium–plant relationship and that Rhizobium leguminosarum can be considered a plant growth-promoting rhizobacterium (PGPR).Key words: Rhizobium, plant growth-promoting rhizobacteria, PGPR, indole-3-acetic acid, cytokinin, roots, auxotrophic mutants.

scholarly journals Quorum Sensing and Indole-3-Acetic Acid Degradation Play a Role in Colonization and Plant Growth Promotion of Arabidopsis thaliana by Burkholderia phytofirmans PsJN

2013 ◽  
Vol 26 (5) ◽  
pp. 546-553 ◽  
Author(s):  
Ana Zúñiga ◽  
María Josefina Poupin ◽  
Raúl Donoso ◽  
Thomas Ledger ◽  
Nicolás Guiliani ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Quorum Sensing ◽  
Plant Growth ◽  
Growth Promotion ◽  
Auxin Signaling ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid ◽  
Burkholderia Phytofirmans Psjn

Although not fully understood, molecular communication in the rhizosphere plays an important role regulating traits involved in plant–bacteria association. Burkholderia phytofirmans PsJN is a well-known plant-growth-promoting bacterium, which establishes rhizospheric and endophytic colonization in different plants. A competent colonization is essential for plant-growth-promoting effects produced by bacteria. Using appropriate mutant strains of B. phytofirmans, we obtained evidence for the importance of N-acyl homoserine lactone-mediated (quorum sensing) cell-to-cell communication in efficient colonization of Arabidopsis thaliana plants and the establishment of a beneficial interaction. We also observed that bacterial degradation of the auxin indole-3-acetic acid (IAA) plays a key role in plant-growth-promoting traits and is necessary for efficient rhizosphere colonization. Wildtype B. phytofirmans but not the iacC mutant in IAA mineralization is able to restore promotion effects in roots of A. thaliana in the presence of exogenously added IAA, indicating the importance of this trait for promoting primary root length. Using a transgenic A. thaliana line with suppressed auxin signaling (miR393) and analyzing the expression of auxin receptors in wild-type inoculated plants, we provide evidence that auxin signaling in plants is necessary for the growth promotion effects produced by B. phytofirmans. The interplay between ethylene and auxin signaling was also confirmed by the response of the plant to a 1-aminocyclopropane-1-carboxylate deaminase bacterial mutant strain.

scholarly journals Indole-3-Acetic Acid and Humic Acid Increase the Bio-Degradation of Diesel Oil in Soil Polluted with Pb and Cd

2021 ◽  
Author(s):  
Amir Hossein Baghaie
Keyword(s):  
Plant Physiology ◽  
Acetic Acid ◽  
Humic Acid ◽  
Plant Growth ◽  
Atomic Absorption Spectroscopy ◽  
Foliar Application ◽  
Organic Amendments ◽  
Diesel Oil ◽  
Soil Samples ◽  
Indole 3 Acetic Acid

Introduction: Soil remediation is one of the most important fields in environmental studies. This study was conducted to investigate the effect of indole-3-acetic acid (IAA) and humic acid (HA) on increasing the bio-degradation of diesel oil in soil polluted with (lead) Pb and cadmium (Cd). Materials and Methods: Treatments included foliar application of IAA (0 (control) and 30 ppm) and soil application of HA (0 (control) and 200 mg/kg soil) in the soil contaminated with Cd (0 (control), 10 and 15 mg/kg soil), Pb (0 (control) and 1600 mg/kg soil), and diesel oil (0 (control), and 8% (W/W)). The sunflower was planted in all soil samples. The plants were harvested after 70 days and Pb and Cd concentrations of plants were measured using Atomic Absorption Spectroscopy. Results: Foliar application of IAA at the rate of 30 mg/l significantly increased the Cd and Pb phytoremediation by 14.8% and 13.4%, respectively. For HA application, it was increased by 11.3% and 10.2%, respectively.  A significant increase was found in degradation percentage of diesel oil in soil by 12.6%, when the soil was treated with 200 mg HA/kg soil. Conclusion: It can be concluded that application of organic amendments such as IAA or HA can be a suitable way for increasing plant growth and increasing plant phytoremediation efficiency, especially in the soil contaminated with diesel oil. However, the phytoremediation efficiency is dependent on the plant physiology and the type of soil pollution that should be considered.

Promotion of plant growth and inhibition of enzymic degradation of indole-3-acetic acid by metabolites of carbofuran, a carbamate insecticide

1977 ◽  
Vol 55 (5) ◽  
pp. 574-579 ◽  
Author(s):  
T. T. Lee
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Inhibitory Action ◽  
Critical Level ◽  
Growth Promotion ◽  
Stem Segment ◽  
Stem Tissue ◽  
Carbamate Insecticide ◽  
Promote Plant Growth ◽  
Indole 3 Acetic Acid

The carbamate insecticide carbofuran (2,2-dimethyl-2,3-dihydrobenzofuranyl-7-N-methyl carbamate) and three of its metabolites (7-hydroxy-2,2-dimethyl-2,3-dihydrobenzofuran (III), 3,7-dihydroxy-2,2-dimethyl-2,3-dihydrobenzofuran (IV), and 3-keto-7-hydroxy-2,2-dimethyl-2,3-dihydrobenzofuran (V)) stimulated growth in the pea stem segment assay in the presence, but not absence, of a low concentration of indole-3-acetic acid (IAA). The metabolites were more active than carbofuran itself. The synergistic effect on growth was specific with IAA since it was not observed in the presence of other auxins.Metabolites III, IV, and V and, to a lesser degree, carbofuran were found to be inhibitory to IAA degradation catalyzed by pea stem tissue or purified horseradish peroxidase (EC 1.11.1.7). Comparison of the relative activities of the compounds in the inhibition of IAA degradation and in the promotion of plant growth suggests a causal relationship. The implication is that carbofuran may promote plant growth through the inhibitory action of its metabolites on enzymic breakdown of IAA, thus preserving a critical level of IAA required for growth promotion.

Monoclonal antibodies to plant growth regulators. II. Indole-3-acetic acid

Planta ◽  
1985 ◽  
Vol 166 (3) ◽  
pp. 389-393 ◽  
Author(s):  
R. Mertens ◽  
J. Eberle ◽  
A. Arnscheidt ◽  
A. Ledebur ◽  
E. W. Weiler
Keyword(s):  
Acetic Acid ◽  
Monoclonal Antibodies ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Indole 3 Acetic Acid
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