Bombyx mori Nucleopolyhedrovirus Bacmid Enabling Rapid Generation of Recombinant Virus by In Vitro Transposition

2015 ◽  
Vol 25 (3) ◽  
pp. 386-392
Author(s):  
Xue Ying Tao ◽  
Jae Young Choi ◽  
Yang-Su Kim ◽  
Seok Hee Lee ◽  
Saes Byeol An ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Recombinant Virus ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Rapid Generation ◽  
In Vitro Transposition

scholarly journals Bombyx mori nucleopolyhedrovirus BmP95 plays an essential role in budded virus production and nucleocapsid assembly

2013 ◽  
Vol 94 (7) ◽  
pp. 1669-1679 ◽  
Author(s):  
Xingwei Xiang ◽  
Yunwang Shen ◽  
Rui Yang ◽  
Lin Chen ◽  
Xiaolong Hu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Virus Production ◽  
Full Length ◽  
Tubular Structures ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Budded Virus ◽  
Conserved Gene ◽  
Fluorescence And Light Microscopy

Bombyx mori nucleopolyhedrovirus (BmNPV) BmP95 is a highly conserved gene that is found in all of the baculovirus genomes sequenced to date and is also found in nudiviruses. To investigate the role of BmP95 in virus infection in vitro, a BmP95 deletion virus (vBmP95-De) was generated by homologous recombination in Escherichia coli. Fluorescence and light microscopy and titration analysis indicated that the BmP95 deletion bacmid led to a defect in production of infectious budded virus (BV). However, deletion of BmP95 did not affect viral DNA replication. Electron microscopy showed that masses of aberrant tubular structures were present in cells transfected with the BmP95 deletion bacmid, indicating that deletion of BmP95 affected assembly of the nucleocapsid. This defect could be rescued by insertion of full-length BmP95 into the polyhedrin locus of the BmP95-knockout bacmid but not the N-terminal domain of BmP95. Together, these results showed that full-length BmP95 is essential for BV production and is required for nucleocapsid assembly.

Systemic and in vitro infection process of Bombyx mori nucleopolyhedrovirus

2004 ◽  
Vol 101 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Md.Masmudur Rahman ◽  
Karumathil P Gopinathan
Keyword(s):  
Bombyx Mori ◽  
Infection Process ◽  
Bombyx Mori Nucleopolyhedrovirus

scholarly journals In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf

Virology ◽  
2006 ◽  
Vol 355 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Susumu Katsuma ◽  
Satoshi Horie ◽  
Takaaki Daimon ◽  
Masashi Iwanaga ◽  
Toru Shimada
Keyword(s):  
Bombyx Mori ◽  
Bombyx Mori Nucleopolyhedrovirus

Early Platelet-Collagen Interactions in Whole Blood and Their Modifications by Aspirin and Dipyridamole Evaluated by a New Method (Basic Wave)

1985 ◽  
Vol 54 (04) ◽  
pp. 799-803 ◽  
Author(s):  
José Luís Pérez-Requejo ◽  
Justo Aznar ◽  
M Teresa Santos ◽  
Juana Vallés
Keyword(s):  
Whole Blood ◽  
Ex Vivo ◽  
Platelet Rich Plasma ◽  
White Blood Cells ◽  
Reversible Aggregation ◽  
Inhibitory Compounds ◽  
Rapid Generation ◽  
Stimulation Of ◽  
Collagen Stimulation

SummaryIt is shown that the supernatant of unstirred whole blood at 37° C, stimulated by 1 μg/ml of collagen for 10 sec, produces a rapid generation of pro and antiaggregatory compounds with a final proaggregatory activity which can be detected for more than 60 min on a platelet rich plasma (PRP) by turbidometric aggregometry. A reversible aggregation wave that we have called BASIC wave (for Blood Aggregation Stimulatory and Inhibitory Compounds) is recorded. The collagen stimulation of unstirred PRP produces a similar but smaller BASIC wave. BASIC’s intensity increases if erythrocytes are added to PRP but decreases if white blood cells are added instead. Aspirin abolishes “ex vivo” the ability of whole blood and PRP to generate BASIC waves and dipyridamole “in vitro” significantly reduces BASIC’s intensity in whole blood in every tested sample, but shows little effect in PRP.

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