Cloning and Characterization of a Glyoxalase I Gene from the Osmotolerant Yeast Candida magnoliae

2011 ◽  
Vol 21 (3) ◽  
pp. 277-283 ◽  
Author(s):  
Eun-Hee Park ◽  
Dae-Hee Lee ◽  
Jin-Ho Seo ◽  
Myoung-Dong Kim
Keyword(s):  
Glyoxalase I ◽  
Candida Magnoliae ◽  
I Gene ◽  
Osmotolerant Yeast

Molecular cloning and characterization of a novel glyoxalase I gene TaGly I in wheat (Triticum aestivum L.)

2009 ◽  
Vol 37 (2) ◽  
pp. 729-735 ◽  
Author(s):  
Fanyun Lin ◽  
Jianhong Xu ◽  
Jianrong Shi ◽  
Hongwei Li ◽  
Bin Li
Keyword(s):  
Triticum Aestivum ◽  
Molecular Cloning ◽  
Triticum Aestivum L ◽  
Glyoxalase I ◽  
I Gene

Characterization of the glyoxalase I gene from the vascular wilt fungus Verticillium dahliae

2006 ◽  
Vol 52 (9) ◽  
pp. 816-822 ◽  
Author(s):  
A Klimes ◽  
M J Neumann ◽  
S J Grant ◽  
K F Dobinson
Keyword(s):  
Verticillium Dahliae ◽  
Glyoxalase I ◽  
Vascular Wilt ◽  
Glyoxalase System ◽  
Enhanced Sensitivity ◽  
Acid Protein ◽  
Gene Homologue ◽  
I Gene ◽  
Amino Acid Protein

A glyoxalase I gene homologue (VdGLO1) was identified in the vascular wilt fungus Verticillium dahliae by sequence tag analysis of genes expressed during resting structure development. The results of the current study show that the gene encodes a putative 345 amino acid protein with high similarity to glyoxalase I, which produces S-D-lactoylglutathione from the toxic metabolic by-product methylglyoxal (MG). Disruption of the V. dahliae gene by Agrobacterium tumefaciens-mediated transformation resulted in enhanced sensitivity to MG. Mycelial growth of disruption mutants was severely reduced in the presence of 5 mmol/L MG. In contrast, spore production in liquid medium was abolished at 1 mmol/L MG, although not at physiologically relevant concentrations of ≤100 µmol/L. In this first report on the characterization of a glyoxalase I gene in a vascular wilt pathogen, we found that disruption of VdGLO1 had no discernable effect on the pathogenicity of V. dahliae. These data suggest that while the glyoxalase system is necessary for effectively dealing with catastrophic levels of MG, under normal conditions of growth and infection, other MG detoxification pathways in V. dahliae are able to compensate for the absence of the glyoxalase system.Key words: verticillium wilt, glycolytic methylglyoxal pathway, 2-oxoaldehydes.

Whole-Genome Sequence Characterization of Primary Auxin-Responsive Aux/IAA Gene Family in Sorghum (Sorghum bicolor L.)

2010 ◽  
Vol 36 (4) ◽  
pp. 688-694
Author(s):  
Yi-Jun WANG ◽  
Yan-Ping LÜ ◽  
Qin XIE ◽  
De-Xiang DENG ◽  
Yun-Long BIAN
Keyword(s):  
Sorghum Bicolor ◽  
Gene Family ◽  
Genome Sequence ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Sequence Characterization ◽  
I Gene

Cloning and Characterization of GmTINY1 Gene in Soybean (Glycine max)

2009 ◽  
Vol 35 (12) ◽  
pp. 2174-2179 ◽  
Author(s):  
Fang HUANG ◽  
Hui HE ◽  
Ying-Jun CHI ◽  
Jun-Yi GAI ◽  
De-Yue YU
Keyword(s):  
Glycine Max ◽  
I Gene

Receptor for advanced glycation end products (RAGE) and glyoxalase I gene polymorphisms in pathological pregnancy

2012 ◽  
Vol 45 (16-17) ◽  
pp. 1409-1414 ◽  
Author(s):  
Anna Germanová ◽  
Alexandra Muravská ◽  
Marie Jáchymová ◽  
Zdeněk Hájek ◽  
Michal Koucký ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Gene Polymorphisms ◽  
Glyoxalase I ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Pathological Pregnancy ◽  
I Gene

scholarly journals Cloning and Characterization of an Arabidopsis thaliana Topoisomerase I Gene

1992 ◽  
Vol 99 (4) ◽  
pp. 1493-1501 ◽  
Author(s):  
Joseph J. Kieber ◽  
Alain F. Tissier ◽  
Ethan R. Signer
Keyword(s):  
Arabidopsis Thaliana ◽  
Topoisomerase I ◽  
I Gene
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