Evidence to Support the Therapeutic Potential of Bacteriophage Kpn5 in Burn Wound Infection Caused by Klebsiella pneumoniae in BALB/c Mice

2010 ◽  
Vol 20 (5) ◽  
pp. 935-941 ◽  
Author(s):  
Seema Kumari
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wound Infection ◽  
Therapeutic Potential ◽  
Burn Wound

Bacteriophage versus antimicrobial agents for the treatment of murine burn wound infection caused by Klebsiella pneumoniae B5055

2011 ◽  
Vol 60 (2) ◽  
pp. 205-210 ◽  
Author(s):  
Seema Kumari ◽  
Kusum Harjai ◽  
Sanjay Chhibber
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wound Infection ◽  
Silver Nitrate ◽  
Antimicrobial Agents ◽  
Phage Therapy ◽  
Burn Wound ◽  
Wound Site ◽  
Two Agents ◽  
Specific Phage ◽  
Percentage Survival

This study was planned to evaluate the efficacy of silver nitrate and gentamicin in the treatment of burn wound infection and to compare it with phage therapy using an isolated and well-characterized Klebsiella-specific phage, Kpn5. A full-thickness burn wound was induced in mice and infected with Klebsiella pneumoniae B5055 via the topical route. Different concentrations of silver nitrate or gentamicin were applied topically daily after establishment of infection. Phage Kpn5 mixed in hydrogel was also applied topically at an m.o.i. of 200 on the burn wound site. The efficacy of these antimicrobial agents was assessed on the basis of percentage survival of infected mice following treatment. The results showed that a single dose of phage Kpn5 resulted in a significant reduction in mortality (P<0.001). Daily applications of silver nitrate and gentamicin at 0.5 % and 1000 mg l−1, respectively, provided significant protection (P<0.001) compared to lower concentrations of the two agents. However, the level of protection given by these two agents was lower than that given by the phage therapy. The results strongly suggest that phage Kpn5 has therapeutic utility in treating burn wound infection in mice as a single topical application of this phage was able to rescue mice from infection caused by K. pneumoniae B5055 in comparison to multiple applications of silver nitrate and gentamicin.

Effective Topical Delivery of H-AgNPs for Eradication of Klebsiella pneumoniae–Induced Burn Wound Infection

2019 ◽  
Vol 20 (5) ◽  
Author(s):  
Sanjay Chhibber ◽  
Vijay Singh Gondil ◽  
Love Singla ◽  
Munish Kumar ◽  
Tanya Chhibber ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wound Infection ◽  
Topical Delivery ◽  
Burn Wound

scholarly journals Topical treatment of Klebsiella pneumoniae B5055 induced burn wound infection in mice using natural products

2010 ◽  
Vol 4 (06) ◽  
pp. 367-377 ◽  
Author(s):  
Seema Kumari ◽  
Kusum Harjai ◽  
Sanjay Chhibber
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wound Infection ◽  
Antimicrobial Agents ◽  
Aloe Vera ◽  
Burn Wound ◽  
Additional Advantage ◽  
Specific Phage ◽  
Aloe Vera Gel ◽  
Therapeutic Value ◽  
Daily Application

Background: Burn wound infection remains the principal cause of death in burn patients. Efficacy of honey and aloe vera gel was evaluated in the treatment of burn wound infection induced with Klebsiella pneumoniae B5055 and their efficacy was compared with an isolated and well-characterized Klebsiella specific phage Kpn5. Methodology: A full thickness burn wound was induced in mice and infected with K. pneumoniae B5055 via topical route. The efficacy of natural antimicrobial agents (honey and aloe vera gel) topically applied daily was compared with the efficacy of phage Kpn5 suspended in hydrogel applied topically a single time on the burn wound. Efficacy of these antimicrobial agents was assessed on the basis of the percentage of infected mice that survived following treatment. Results: In comparison to untreated control mice, those treated with a single dose of phage Kpn5 at MOI of 200 showed significant reduction in mortality (P < 0.001). Daily application of honey and aloe vera gel provided significant protection (P < 0.001), but in combination with phage, no additional advantage was observed (P > 0.05) compared to the use of honey and aloe vera gel alone. Conclusions: The results of this study strongly suggest that phage Kpn5 has therapeutic value in treating burn wound infection in mice as a single topical application of this phage was able to rescue mice from infection caused by K. pneumoniae B5055 in comparison to multiple applications of honey and aloe vera gel.

scholarly journals The Microbial Profile and Antibiotic Susceptibility in Burn Wound Infection: A Hospital Based Study

2020 ◽  
pp. 76-86
Author(s):  
P. Denen Akaa ◽  
C. N. Ahachi ◽  
E. Ojo ◽  
G. T. Jombo ◽  
S. Osuji ◽  
...  
Keyword(s):  
Wound Infection ◽  
Antibiotic Susceptibility ◽  
Continuous Process ◽  
Total Body Surface Area ◽  
Antibiotics Resistance ◽  
Burn Wound ◽  
Wound Swab ◽  
Microbial Profile ◽  
Sensitivity Pattern ◽  
Culture And Sensitivity

Aim: To determine spectrum of bacteria infecting burn wound and its antibiotic susceptibility at Benue State University Teaching Hospital (BSUTH). Study Design: A one year prospective study of the microbial profile in burn wound infection at BSUTH, Makurdi. Methodology: All patients admitted from August 2018 to July 2019 with burn wound who had wound swab microscopy culture and sensitivity (MCS) after thorough cleaning of the wound were included in the study. Data collected included the Age, Sex, Occupation, Total Body Surface Area (TBSA) of burn, injuring agent, type of burn, bacteria cultured from the wound, antibiotic susceptibility, number of days of admission and outcome of treatment. Results: Out of the 63 patients admitted, flame was the predominant injuring agent seen in 54 (85.7%) patients. The age group 21 – 30 years was the most affected, 27 constituting 42.9%. Burn wound infection occurred in 25(39.7%) patients from whom 33 bacterial isolates were recovered. Gram negative organisms Pseudomonas species and Proteus species were the commonest bacteria isolated constituting 21.21% each. Other isolates were Staphylococcus aurous 36.36%, Escherichia coli 15.15%, Klebsiella species and Staphylococcus saprophyticus (each 3.03%,). There was multiple bacteria colonization of wounds and antibiotics resistance. There was no gender difference in the burn wound infection, P =0.7819. Conclusion: The sensitivity pattern showed many of the bacteria to be resistant to commonly administered antibiotics but sensitivity patterns got are important for empirical antibiotics prescription when patients come with glaring sepsis and are waiting for wound swab microscopy culture and sensitivity. Studying the microbial profile with antibiotic resistance pattern in burn’s wound infection should be a continuous process in all burn units/departments. This will help to formulate and modify at regular intervals, a hospital/departmental antibiotic policy according to the present microbial pattern in the respective burns unit.    

scholarly journals Extended Spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from Burn Wound Infections

2016 ◽  
Vol 5 (9) ◽  
pp. 400-404 ◽  
Author(s):  
Khulood Abdulkareen Hussein ◽  
Zainab Alag Hasan ◽  
Utoor Talib Jassim ◽  
Abdulameer Abdullah Al-Mussawi
Keyword(s):  
Klebsiella Pneumoniae ◽  
Wound Infections ◽  
Burn Wound ◽  
Extended Spectrum
Sign in / Sign up

Export Citation Format

Share Document