scholarly journals Evaluation of Nano-curcumin effects on expression levels of virulence genes and biofilm production of multidrug-resistant Pseudomonas aeruginosa isolated from burn wound infection in Tehran, Iran

2019 ◽  
Vol Volume 12 ◽  
pp. 2223-2235 ◽  
Author(s):  
Aref Shariati ◽  
Elham Asadian ◽  
Fatemeh Fallah ◽  
Taher Azimi ◽  
Ali Hashemi ◽  
...  
Keyword(s):  
Wound Infection ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Burn Wound ◽  
Expression Levels ◽  
Biofilm Production

scholarly journals Prevalence of Multidrug Resistant (MDR) <i>Proteus</i> spp. in Burn Wound Infection of a Tertiary Care Hospital, Rajshahi

2021 ◽  
Vol 6 (2) ◽  
pp. 65
Author(s):  
Anika Tasnim ◽  
Md. Shah Alam ◽  
Md. Abdullah Yusuf ◽  
Fazlay Akbar Khan ◽  
Jannatul Ferdose ◽  
...  
Keyword(s):  
Wound Infection ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Multidrug Resistant ◽  
Care Hospital ◽  
Burn Wound

scholarly journals Investigation of the association of virulence genes and biofilm production with infection and bacterial colonization processes in multidrug-resistant Acinetobacter spp.

2021 ◽  
Vol 93 (suppl 3) ◽  
Author(s):  
ADRIANA MARIA C.M. DA SILVA ◽  
SÉRGIO D. COSTA JÚNIOR ◽  
JAILTON L.C. LIMA ◽  
JOSÉ LUCIANO B. DE FARIAS FILHO ◽  
ISABELLA M.F. CAVALCANTI ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Bacterial Colonization ◽  
Multidrug Resistant ◽  
Biofilm Production ◽  
Acinetobacter Spp

scholarly journals Effect of ZnO nanoparticles on methicillin, vancomycin, linezolid resistance and biofilm formation in Staphylococcus aureus isolates

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Wedad M. Abdelraheem ◽  
Rasha M. M. Khairy ◽  
Alaa I. Zaki ◽  
Shaimaa H. Zaki
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Genes ◽  
Biofilm Formation ◽  
Formation Rate ◽  
Microtiter Plate ◽  
Multidrug Resistant ◽  
P Value ◽  
Burn Wound ◽  
Zno Nps ◽  
Expression Levels

Abstract Background Multidrug resistant (MDR) and biofilm producing Staphylococcus aureus strains are usually associated with serious infections. This study aimed to evaluate the antibacterial and antibiofilm-formation effects of zinc oxide nanoparticles (ZnO-NPs) against staphylococcus aureus (S. aureus) isolates. Methods A total of 116 S. aureus isolates were recovered from 250 burn wound samples. The antimicrobial/antibiofilm effects of ZnO-NPs against methicillin, vancomycin and linezolid resistant S. aureus (MRSA, VRSA and LRSA) isolates were examined using phenotypic and genotypic methods. The minimum inhibitory concentration (MIC) of ZnO-NPs was determined by microdilution method. The effects of sub-MIC concentrations of ZnO-NPs on biofilm formation and drug resistance in S. aureus were determined by the microtiter plate method. The change in the expression levels of the biofilm encoding genes and resistance genes in S. aureus isolates after treatment with ZnO-NPs was assessed by real time reverse transcriptase PCR (rt-PCR). Results MICs of ZnO-NPs in S. aureus isolates were (128–2048 µg/ml). The sub-MIC of ZnO-NPs significantly reduced biofilm formation rate (the highest inhibition rate was 76.47% at 1024  µg/ml) and the expression levels of biofilm genes (ica A, ica D and fnb A) with P < 0.001. Moreover, Sub-MIC of ZnO-NPs significantly reduced the rates of MRSA from 81.9 (95 isolates) to 13.30% (15 isolates), VRSA from 33.60 (39 isolates) to 0% and LARSA from 29.30 (34) to 0% as well as the expression levels of resistance genes (mec A, van A and cfr) with P value < 0.001. Conclusion ZnO-NPs can be used as antibiofilm and potent antimicrobial against MRSA, VRSA and LRSA isolates.

Diversity of virulence genes in multidrug resistant Pseudomonas aeruginosa isolated from burn wound infections

2018 ◽  
Vol 115 ◽  
pp. 251-256 ◽  
Author(s):  
Fakhri Haghi ◽  
Habib Zeighami ◽  
Arefeh Monazami ◽  
Farnaz Toutouchi ◽  
Shima Nazaralian ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Wound Infections ◽  
Burn Wound

Risk Factors for Nosocomial Burn Wound Infection Caused by Multidrug Resistant Acinetobacter baumannii

2014 ◽  
Vol 35 (1) ◽  
pp. e73-e80 ◽  
Author(s):  
Recep Tekin ◽  
Tuba Dal ◽  
Fatma Bozkurt ◽  
Özcan Deveci ◽  
Ylmaz Palanc ◽  
...  
Keyword(s):  
Risk Factors ◽  
Wound Infection ◽  
Acinetobacter Baumannii ◽  
Multidrug Resistant ◽  
Burn Wound

scholarly journals Presence of β-Lactamase-producing Enterobacterales and Salmonella Isolates in Marine Mammals

2021 ◽  
Vol 22 (11) ◽  
pp. 5905
Author(s):  
Olivia M. Grünzweil ◽  
Lauren Palmer ◽  
Adriana Cabal ◽  
Michael P. Szostak ◽  
Werner Ruppitsch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Marine Mammals ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Healthcare Sector ◽  
Whole Genome ◽  
E Coli ◽  
High Prevalence ◽  
Lactamase Gene ◽  
Sequence Types

Marine mammals have been described as sentinels of the health of marine ecosystems. Therefore, the aim of this study was to investigate (i) the presence of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Enterobacterales, which comprise several bacterial families important to the healthcare sector, as well as (ii) the presence of Salmonella in these coastal animals. The antimicrobial resistance pheno- and genotypes, as well as biocide susceptibility of Enterobacterales isolated from stranded marine mammals, were determined prior to their rehabilitation. All E. coli isolates (n = 27) were screened for virulence genes via DNA-based microarray, and twelve selected E. coli isolates were analyzed by whole-genome sequencing. Seventy-one percent of the Enterobacterales isolates exhibited a multidrug-resistant (MDR) pheno- and genotype. The gene blaCMY (n = 51) was the predominant β-lactamase gene. In addition, blaTEM-1 (n = 38), blaSHV-33 (n = 8), blaCTX-M-15 (n = 7), blaOXA-1 (n = 7), blaSHV-11 (n = 3), and blaDHA-1 (n = 2) were detected. The most prevalent non-β-lactamase genes were sul2 (n = 38), strA (n = 34), strB (n = 34), and tet(A) (n = 34). Escherichia coli isolates belonging to the pandemic sequence types (STs) ST38, ST167, and ST648 were identified. Among Salmonella isolates (n = 18), S. Havana was the most prevalent serotype. The present study revealed a high prevalence of MDR bacteria and the presence of pandemic high-risk clones, both of which are indicators of anthropogenic antimicrobial pollution, in marine mammals.

scholarly journals Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Quan Li ◽  
Jian Yin ◽  
Zheng Li ◽  
Zewei Li ◽  
Yuanzhao Du ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Antimicrobial Susceptibility ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Resistance Rate ◽  
Economic Losses ◽  
Production Chain ◽  
Antimicrobial Resistance Genes

AbstractSalmonella is an important food-borne pathogen associated with public health and high economic losses. To investigate the prevalence and the characteristics of Salmonella in a pig slaughterhouse in Yangzhou, a total of 80 Salmonella isolates were isolated from 459 (17.43%) samples in 2016–2017. S. Derby (35/80, 43.75%) was the most prevalent, followed by S. Rissen (16/80, 20.00%) and S. Newlands (11/80, 13.75%). The highest rates of susceptibility were observed to cefoxitin (80/80, 100.0%) and amikacin (80/80, 100.0%), followed by aztreonam (79/80, 98.75%) and nitrofurantoin (79/80, 98.75%). The highest resistance rate was detected for tetracycline (65/80, 81.25%), followed by ampicillin (60/80, 75.00%), bactrim (55/80, 68.75%), and sulfisoxazole (54/80, 67.50%). Overall, 91.25% (73/80) of the isolates were resistant to at least one antibiotic, while 71.25% (57/80) of the isolate strains were multidrug resistant in the antimicrobial susceptibility tested. In addition, 86.36% (19/22) of the 22 antimicrobial resistance genes in the isolates were identified. Our data indicated that the resistance to certain antimicrobials was significantly associated, in part, with antimicrobial resistance genes. Furthermore, 81.25% (65/80) isolates harbored the virulence gene of mogA, of which 2 Salmonella Typhimurium isolates carried the mogA, spvB and spvC virulence genes at the same time. The results showed that swine products in the slaughterhouse were contaminated with multidrug resistant Salmonella commonly, especially some isolates carry the spv virulence genes. The virulence genes might facilitate the dissemination of the resistance genes to consumers along the production chain, suggesting the importance of controlling Salmonella during slaughter for public health.

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