Polyphosphate Kinase Affects Oxidative Stress Response by Modulating cAMP Receptor Protein and rpoS Expression in Salmonella Typhimurium

2009 ◽  
Vol 19 (12) ◽  
pp. 1527-1535 ◽  
Author(s):  
Yuanyuan Cheng
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Salmonella Typhimurium ◽  
Oxidative Stress Response ◽  
Receptor Protein ◽  
Polyphosphate Kinase ◽  
Camp Receptor Protein ◽  
Camp Receptor

scholarly journals Mutations that affect transcription and cyclic AMP-CRP regulation of the adenylate cyclase gene (cya) of Salmonella typhimurium.

Genetics ◽  
1990 ◽  
Vol 125 (4) ◽  
pp. 719-727
Author(s):  
J P Fandl ◽  
L K Thorner ◽  
S W Artz
Keyword(s):  
Salmonella Typhimurium ◽  
Binding Site ◽  
Minimal Medium ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Multiple Promoters ◽  
P2 Promoter ◽  
Camp Receptor ◽  
Insertion Mutations

Abstract We studied the expression of the cya promoter(s) in cya-lac fusion strains of Salmonella typhimurium and demonstrated cAMP receptor protein (CRP)-dependent repression by cAMP. Expression of cya was reduced about fourfold in cultures grown in acetate minimal medium as compared to cultures grown in glucose-6-phosphate minimal medium. Expression of cya was also reduced about fourfold by addition of 5 mM cAMP to cultures grown in glucose minimal medium. We constructed in vitro deletion and insertion mutations altering a major cya promoter (P2) and a putative CRP binding site overlapping P2. These mutations were recombined into the chromosome by allele replacement with M13mp::cya recombinant phages and the regulation of the mutant promoters was analyzed. A 4-bp deletion of the CRP binding site and a 4-bp insertion in this site nearly eliminated repression by cAMP. A mutant with the P2 promoter and the CRP binding site both deleted exhibited an 80% reduction in cya expression; the 20% residual expression was insensitive to cAMP repression. This mutant retained a Cya+ phenotype. Taken together, the results establish that the cya gene is transcribed from multiple promoters one of which, P2, is negatively regulated by the cAMP-CRP complex. Correction for the contribution to transcription by the cAMP-CRP nonregulated cya promoters indicates that the P2 promoter is repressed at least eightfold by cAMP-CRP.

Role of a Burkholderia pseudomallei polyphosphate kinase in an oxidative stress response, motilities, and biofilm formation

2010 ◽  
Vol 48 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Suda Tunpiboonsak ◽  
Rungrawee Mongkolrob ◽  
Kaniskul Kitudomsub ◽  
Phawatwaristh Thanwatanaying ◽  
Witcha Kiettipirodom ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Biofilm Formation ◽  
Burkholderia Pseudomallei ◽  
Oxidative Stress Response ◽  
Polyphosphate Kinase

scholarly journals DdrI, a cAMP Receptor Protein Family Member, Acts as a Major Regulator for Adaptation ofDeinococcus radioduransto Various Stresses

2018 ◽  
Vol 200 (13) ◽  
Author(s):  
Laura Meyer ◽  
Geneviève Coste ◽  
Suzanne Sommer ◽  
Jacques Oberto ◽  
Fabrice Confalonieri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Division ◽  
Heat Shock ◽  
Receptor Protein ◽  
Dependent Manner ◽  
Central Metabolism ◽  
Camp Receptor Protein ◽  
Content Type ◽  
Dna Damaging Agents ◽  
Camp Receptor

ABSTRACTTheDNAdamageresponseddrIgene encodes a transcription regulator belonging to the cAMP receptor protein (CRP) family. Cells devoid of the DdrI protein exhibit a pleiotropic phenotype, including growth defects and sensitivity to DNA-damaging agents and to oxidative stress. Here, we show that the absence of the DdrI protein also confers sensitivity to heat shock treatment, and several genes involved in heat shock response were shown to be upregulated in a DdrI-dependent manner. Interestingly, expression of theEscherichia coliCRP partially compensates for the absence of the DdrI protein. Microscopic observations of ΔddrImutant cells revealed an increased proportion of two-tetrad and anucleated cells in the population compared to the wild-type strain, indicating that DdrI is crucial for the completion of cell division and/or chromosome segregation. We show that DdrI is also involved in the megaplasmid MP1 stability and in efficient plasmid transformation by facilitating the maintenance of the incoming plasmid in the cell. Thein silicoprediction of putative DdrI binding sites in theD. radioduransgenome suggests that hundreds of genes, belonging to several functional groups, may be regulated by DdrI. In addition, the DdrI protein absolutely requires cAMP forin vitrobinding to specific target sequences, and it acts as a dimer. All these data underline the major role of DdrI inD. radioduransphysiology under normal and stress conditions by regulating, both directly and indirectly, a cohort of genes involved in various cellular processes, including central metabolism and specific responses to diverse harmful environments.IMPORTANCEDeinococcus radioduranshas been extensively studied to elucidate the molecular mechanisms responsible for its exceptional ability to withstand lethal effects of various DNA-damaging agents. A complex network, including efficient DNA repair, protein protection against oxidation, and diverse metabolic pathways, plays a crucial role for its radioresistance. The regulatory networks orchestrating these various pathways are still missing. Our data provide new insights into the crucial contribution of the transcription factor DdrI for theD. radioduransability to withstand harmful conditions, including UV radiation, mitomycin C treatment, heat shock, and oxidative stress. Finally, we highlight that DdrI is also required for accurate cell division, for maintenance of plasmid replicons, and for central metabolism processes responsible for the overall cell physiology.

Role of Salmonella Typhimurium small RNAs RyhB-1 and RyhB-2 in the oxidative stress response

2014 ◽  
Vol 165 (1) ◽  
pp. 30-40 ◽  
Author(s):  
Iván L. Calderón ◽  
Eduardo H. Morales ◽  
Bernardo Collao ◽  
Paulina F. Calderón ◽  
Catalina A. Chahuán ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Salmonella Typhimurium ◽  
Small Rnas ◽  
Oxidative Stress Response
Sign in / Sign up

Export Citation Format

Share Document