Molecular Cloning and Expression of the Trichoderma harzianum C4 Endo-β-1,4-Xylanase Gene in Saccharomyces cerevisiae

2009 ◽  
Author(s):  
Dae Kyun Chung
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Cloning ◽  
Trichoderma Harzianum ◽  
Cloning And Expression ◽  
Xylanase Gene

Molecular Cloning and Expression of β-1,4-endoxylanase Gene from Chaetomium cupreum in Yeast Saccharomyces cerevisiae

2013 ◽  
Vol 779-780 ◽  
pp. 191-194
Author(s):  
Hai Yan Zhang ◽  
Wen Rao Li ◽  
Min Li
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Heterologous Expression ◽  
Molecular Mass ◽  
Molecular Cloning ◽  
Cloning And Expression ◽  
Cdna Fragment ◽  
Gene Encoding ◽  
Calculated Molecular Mass

The gene encoding an endo-β-1,4-xylanase (XynCC) fromchaetomium cupreumwas amplified using PCR. The nucleotide sequence of a 690 bp cDNA fragment was determined. Based on the nucleotide sequence, calculated molecular mass of the enzyme was 24.7 kDa. The XynCC gene was inserted into the pYES2 vector and transferred into the cells ofS. cerevisiaeH158 for heterologous expression.

Cloning and expression of an Aspergillus kawachii endo-1,4-β-xylanase gene in Saccharomyces cerevisiae

1995 ◽  
Vol 28 (5) ◽  
pp. 467-473 ◽  
Author(s):  
Johan M. Crous ◽  
Isak S. Pretorius ◽  
Willem H. van Zyl
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cloning And Expression ◽  
Xylanase Gene ◽  
Aspergillus Kawachii

Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli

1990 ◽  
Vol 34 (1) ◽  
Author(s):  
J. Bhalerao ◽  
A.H. Patki ◽  
M. Bhave ◽  
I. Khurana ◽  
D.N. Deobagkar
Keyword(s):  
Escherichia Coli ◽  
Molecular Cloning ◽  
Cloning And Expression ◽  
Xylanase Gene
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