Development and Validation of a Solid-Phase Radioimmunoassay for Measuring Progesterone and Testosterone in Octopus Gonad Extracts

Malacologia ◽  
2013 ◽  
Vol 56 (1 & 2) ◽  
pp. 121-134 ◽  
Author(s):  
Omar Hernando Avila-Poveda ◽  
Ruben Cornelio Montes-Pérez ◽  
Francisco Benitez-Villalobos ◽  
Carlos Rosas
Keyword(s):  
Solid Phase ◽  
Solid Phase Radioimmunoassay ◽  
Development And Validation

Radioimmunoassay of Fibrinogen and Its Proteolysis Products

1968 ◽  
Vol 20 (01/02) ◽  
pp. 001-006 ◽  
Author(s):  
K. J Catt ◽  
J Hirsh ◽  
D. J Castelan ◽  
H. D Niall ◽  
G. W Tregear
Keyword(s):  
Solid Phase ◽  
Solid Phase Radioimmunoassay ◽  
Radioimmunoassay Method

SummaryThe solid-phase radioimmunoassay method has been applied to the measurement of fibrinogen. The method is extremely sensitive, being able to detect fibrinogen concentrations as low as 10 ng/ml. The immunoreactivity of fibrinogen proteolysis products differs from that of native fibrinogen, early proteolysis products showing enhanced immunoreactivity which decreases progressively with further digestion.

Solid-Phase Radioimmunoassay in Antibody-Coated Tubes

Science ◽  
1967 ◽  
Vol 158 (3808) ◽  
pp. 1570-1572 ◽  
Author(s):  
K. Catt ◽  
G. W. Tregear
Keyword(s):  
Solid Phase ◽  
Solid Phase Radioimmunoassay

scholarly journals UHPLC-ESI-MS/MS Quantification of Relevant Substrates and Metabolites of the Kynurenine Pathway Present in Serum and Peritoneal Fluid from Gastric Cancer Patients—Method Development and Validation

2021 ◽  
Vol 22 (13) ◽  
pp. 6972
Author(s):  
Ilona Sadok ◽  
Katarzyna Jędruchniewicz ◽  
Karol Rawicz-Pruszyński ◽  
Magdalena Staniszewska
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Peritoneal Fluid ◽  
Method Development ◽  
Solid Phase ◽  
Kynurenine Pathway ◽  
Serum Samples ◽  
Esi Ms ◽  
Gastric Cancer Patients ◽  
Development And Validation

Metabolites and enzymes involved in the kynurenine pathway (KP) are highly promising targets for cancer treatment, including gastrointestinal tract diseases. Thus, accurate quantification of these compounds in body fluids becomes increasingly important. The aim of this study was the development and validation of the UHPLC-ESI-MS/MS methods for targeted quantification of biologically important KP substrates (tryptophan and nicotinamide) and metabolites(kynurenines) in samples of serum and peritoneal fluid from gastric cancer patients. The serum samples were simply pretreated with trichloroacetic acid to precipitate proteins. The peritoneal fluid was purified by solid-phase extraction before analysis. Validation was carried out for both matrices independently. Analysis of the samples from gastric cancer patients showed different accumulations of tryptophan and its metabolites in different biofluids of the same patient. The protocols will be used for the evaluation of tryptophan and kynurenines in blood and peritoneal fluid to determine correlation with the clinicopathological status of gastric cancer or the disease’s prognosis.

scholarly journals Development and validation of a bioanalytical LC-UV method with solid-phase extraction for determination of valproic acid in saliva

2012 ◽  
Vol 62 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Jasmina Tonic-Ribarska ◽  
Arlinda Haxhiu ◽  
Zoran Sterjev ◽  
Gordana Kiteva ◽  
Ljubica Suturkova ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Human Saliva ◽  
Hplc Method ◽  
Therapeutic Monitoring ◽  
Phase Extraction ◽  
Free Drug Concentration ◽  
Development And Validation

Development and validation of a bioanalytical LC-UV method with solid-phase extraction for determination of valproic acid in saliva A bioanalytical HPLC method with UV detection for the determination of the antiepileptic drug valproic acid in human saliva has been developed and validated. Saliva represents an alternative matrix for therapeutic monitoring of antiepileptic drugs due to the increasing interest in free drug concentration. The proposed method involved solid-phase extraction for sample preparation and yielded very good mean recoveries of 99.4 % and 97.9 % for valproic acid and IS, respectively. The calibration function for valproic acid was linear over the concentration range of 1.0-50.0 μg mL-1 (R2 = 0.9989). Within-run and between-run precision and accuracy were studied at four concentrations and RSDs were less than 7.3 and 2.2 %, while accuracy values were higher than 96.8 and 97.5 %, respectively. The described method provides sensitivity, linearity, precision, accuracy and is suitable for analyses of valproic acid in saliva samples.

A simple solid-phase radioimmunoassay for the measurement of IgG secreted in vitro by human lymphocytes

1979 ◽  
Vol 29 (3) ◽  
pp. 263-270 ◽  
Author(s):  
S. Romagnani ◽  
G.F. Del Prete ◽  
Grazia M. Giudizi ◽  
F. Almerigogna ◽  
M. Ricci
Keyword(s):  
Solid Phase ◽  
Human Lymphocytes ◽  
Solid Phase Radioimmunoassay

Solid-Phase Radioimmunoassay of Steroids

1970 ◽  
pp. 127-147
Author(s):  
Stephen A. Tillson ◽  
Ian H. Thorneycroft ◽  
Guy E. Abraham ◽  
Rex J. Scaramuzzi ◽  
Burton V. Caldwell
Keyword(s):  
Solid Phase ◽  
Solid Phase Radioimmunoassay
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