Development and validation of an HPLC method for the simultaneous determination of tocopherols, tocotrienols and carotenoids in cereals after solid-phase extraction

2011 ◽  
Vol 34 (12) ◽  
pp. 1375-1382 ◽  
Author(s):  
Maria N. Irakli ◽  
Victoria F. Samanidou ◽  
Ioannis N. Papadoyannis
Keyword(s):  
Solid Phase Extraction ◽  
Simultaneous Determination ◽  
Solid Phase ◽  
Hplc Method ◽  
Phase Extraction ◽  
Development And Validation

scholarly journals Development and validation of a bioanalytical LC-UV method with solid-phase extraction for determination of valproic acid in saliva

2012 ◽  
Vol 62 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Jasmina Tonic-Ribarska ◽  
Arlinda Haxhiu ◽  
Zoran Sterjev ◽  
Gordana Kiteva ◽  
Ljubica Suturkova ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Human Saliva ◽  
Hplc Method ◽  
Therapeutic Monitoring ◽  
Phase Extraction ◽  
Free Drug Concentration ◽  
Development And Validation

Development and validation of a bioanalytical LC-UV method with solid-phase extraction for determination of valproic acid in saliva A bioanalytical HPLC method with UV detection for the determination of the antiepileptic drug valproic acid in human saliva has been developed and validated. Saliva represents an alternative matrix for therapeutic monitoring of antiepileptic drugs due to the increasing interest in free drug concentration. The proposed method involved solid-phase extraction for sample preparation and yielded very good mean recoveries of 99.4 % and 97.9 % for valproic acid and IS, respectively. The calibration function for valproic acid was linear over the concentration range of 1.0-50.0 μg mL-1 (R2 = 0.9989). Within-run and between-run precision and accuracy were studied at four concentrations and RSDs were less than 7.3 and 2.2 %, while accuracy values were higher than 96.8 and 97.5 %, respectively. The described method provides sensitivity, linearity, precision, accuracy and is suitable for analyses of valproic acid in saliva samples.

Development and validation of an HPLC method for determination of amphotericin B in plasma and sputum involving solid phase extraction

1997 ◽  
Vol 46 (11-12) ◽  
pp. 641-646 ◽  
Author(s):  
M. A. Campanero ◽  
A. M. Zamarreño ◽  
M. Diaz ◽  
M. C. Dios-Vieitez ◽  
J. R. Azanza
Keyword(s):  
Solid Phase Extraction ◽  
Amphotericin B ◽  
Solid Phase ◽  
Hplc Method ◽  
Phase Extraction ◽  
Development And Validation
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