Evaluation of Anonymous and Expressed Sequence Tag-Derived Polymorphic Microsatellite Markers in the Tobacco Budworm,Heliothis virescens(Lepidoptera: Noctuidae)

2011 ◽  
Vol 36 (3) ◽  
pp. 287-294 ◽  
Author(s):  
O. P. Perera ◽  
C. A. Blanco ◽  
L. Ballard ◽  
K. L. Silva-Brandão ◽  
F. A. Domingues ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Heliothis Virescens ◽  
Expressed Sequence Tag ◽  
Tobacco Budworm ◽  
Polymorphic Microsatellite ◽  
Lepidoptera Noctuidae ◽  
Expressed Sequence

Use of expressed sequence tag microsatellite markers for population genetic research of Helicoverpa armigera (Lepidoptera: Noctuidae) from India

2015 ◽  
Vol 148 (2) ◽  
pp. 187-199
Author(s):  
Thiruvengadam Venkatesan ◽  
Vaddi Sridhar ◽  
Yan R. Tomason ◽  
Sushil Kumar Jalali ◽  
Gajanan T. Behere ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Microsatellite Markers ◽  
Population Genetic ◽  
Expressed Sequence Tag ◽  
Genetic Research ◽  
Western India ◽  
Diversity Analysis ◽  
Lepidoptera Noctuidae ◽  
Expressed Sequence

AbstractCotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is a serious pest of several crops throughout the world, representing millions of United States of America dollars worth of damage. This pest can adapt to various cropping systems in a wide geographical range and has high migratory potential. It features high fecundity and can develop resistance to almost all insecticides used for its management. Several investigations to develop microsatellite markers for H. armigera have not been successful because of the paucity of microsatellites in the lepidopteran genome. As well, collections of H. armigera from cotton fields of southern and western India were not yet studied for molecular genetic diversity. The current study aimed to screen publicly available expressed sequence tag resources for simple sequence repeats and assess their potential as DNA markers for assessment of gene flow between collections of southern and western India. We identified 30 polymorphic microsatellites for potential use in diversity analysis of H. armigera collections. Genetic diversity analysis revealed that the collections were widely diverse with population differentiation index (Fst) of 0.17. Furthermore, gene flow analysis revealed a mean frequency of private alleles of 11% within the collections. The microsatellite resources we developed could be widely used for molecular diversity or population genetic research involving this important pest of cotton and food crops.

scholarly journals Ecdysteroidogenesis in Heliothis virescens (Lepidoptera: Noctuidae): Recombinant Prothoracicotropic Hormone and Brain Extract Show Comparable Effects

2019 ◽  
Vol 19 (3) ◽  
Author(s):  
Marisa Nardiello ◽  
Rosanna Salvia ◽  
Andrea Scala ◽  
Carmen Scieuzo ◽  
Sabino Aurelio Bufo ◽  
...  
Keyword(s):  
Heliothis Virescens ◽  
Complex Mixture ◽  
Prothoracic Gland ◽  
Tobacco Budworm ◽  
Brain Extract ◽  
In Vitro Tests ◽  
Prothoracicotropic Hormone ◽  
Lepidoptera Noctuidae

Abstract Prothoracicotropic hormone (PTTH) is a neuropeptide that triggers a cascade of events within the prothoracic gland (PG) cells, leading to the activation of all the crucial enzymes involved in ecdysone biosynthesis, the main insect steroid hormone. Studies concerning ecdysteroidogenesis predicted PTTH action using brain extract (BE), consisting in a complex mixture in which some components positively or negatively interfere with PTTH-stimulated ecdysteroidogenesis. Consequently, the integration of these opposing factors in steroidogenic tissues leads to a complex secretory pattern. A recombinant form of prothoracicotropic hormone (rPTTH) from the tobacco budworm Heliothis virescens (F.) (Lepidoptera: Noctuidae) was expressed and purified to perform in vitro tests in a standard and repeatable manner. A characterization of rPTTH primary and secondary structures was performed. The ability of rPTTH and H. virescens BE to stimulate ecdysteroidogenesis was investigated on the third day of fifth larval stage. rPTTH activity was compared with the BE mixture by enzyme immunoassay and western blot, revealing that they equally stimulate the production of significant amount of ecdysone, through a transduction cascade that includes the TOR pathway, by the phosphorylation of 4E binding protein (4E-BP) and S6 kinase (S6K), the main targets of TOR protein. The results of these experiments suggest the importance of obtaining a functional pure hormone to perform further studies, not depending on the crude brain extract, composed by different elements and susceptible to different uncontrollable variables.

Mining of expressed sequence tag libraries of cacao for microsatellite markers using five computational tools

2009 ◽  
Vol 88 (2) ◽  
pp. 217-225 ◽  
Author(s):  
Aikkal Riju ◽  
M. K. Rajesh ◽  
P. T. P. Fasila Sherin ◽  
A. Chandrasekar ◽  
S. Elain Apshara ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Expressed Sequence Tag ◽  
Computational Tools ◽  
Expressed Sequence
Sign in / Sign up

Export Citation Format

Share Document