Use of Microsatellite Markers Derived from Genomic and Expressed Sequence Tag (EST) Data to Identify Commercial Watermelon Cultivars

2015 ◽  
Vol 33 (5) ◽  
pp. 737-750 ◽  
Author(s):  
Yong-Sham Kwon ◽  
Jee-Hwa Hong ◽  
Du-Hyun Kim ◽  
Do-Hoon Kim
Keyword(s):  
Microsatellite Markers ◽  
Expressed Sequence Tag ◽  
Expressed Sequence

Use of expressed sequence tag microsatellite markers for population genetic research of Helicoverpa armigera (Lepidoptera: Noctuidae) from India

2015 ◽  
Vol 148 (2) ◽  
pp. 187-199
Author(s):  
Thiruvengadam Venkatesan ◽  
Vaddi Sridhar ◽  
Yan R. Tomason ◽  
Sushil Kumar Jalali ◽  
Gajanan T. Behere ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Microsatellite Markers ◽  
Population Genetic ◽  
Expressed Sequence Tag ◽  
Genetic Research ◽  
Western India ◽  
Diversity Analysis ◽  
Lepidoptera Noctuidae ◽  
Expressed Sequence

AbstractCotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is a serious pest of several crops throughout the world, representing millions of United States of America dollars worth of damage. This pest can adapt to various cropping systems in a wide geographical range and has high migratory potential. It features high fecundity and can develop resistance to almost all insecticides used for its management. Several investigations to develop microsatellite markers for H. armigera have not been successful because of the paucity of microsatellites in the lepidopteran genome. As well, collections of H. armigera from cotton fields of southern and western India were not yet studied for molecular genetic diversity. The current study aimed to screen publicly available expressed sequence tag resources for simple sequence repeats and assess their potential as DNA markers for assessment of gene flow between collections of southern and western India. We identified 30 polymorphic microsatellites for potential use in diversity analysis of H. armigera collections. Genetic diversity analysis revealed that the collections were widely diverse with population differentiation index (Fst) of 0.17. Furthermore, gene flow analysis revealed a mean frequency of private alleles of 11% within the collections. The microsatellite resources we developed could be widely used for molecular diversity or population genetic research involving this important pest of cotton and food crops.

Mining of expressed sequence tag libraries of cacao for microsatellite markers using five computational tools

2009 ◽  
Vol 88 (2) ◽  
pp. 217-225 ◽  
Author(s):  
Aikkal Riju ◽  
M. K. Rajesh ◽  
P. T. P. Fasila Sherin ◽  
A. Chandrasekar ◽  
S. Elain Apshara ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Expressed Sequence Tag ◽  
Computational Tools ◽  
Expressed Sequence

scholarly journals Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee

2011 ◽  
Vol 46 (12) ◽  
pp. 1650-1656 ◽  
Author(s):  
Gabriella Santos Pereira ◽  
Lilian Padilha ◽  
Edila Vilela Resende Von Pinho ◽  
Rita de Kássia Siqueira Teixeira ◽  
Carlos Henrique Siqueira de Carvalho ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Ssr Markers ◽  
Expressed Sequence Tag ◽  
Leaf Miner ◽  
Est Database ◽  
Ssr Loci ◽  
Leucoptera Coffeella ◽  
Coffee Leaf Miner ◽  
Expressed Sequence ◽  
Simple Sequence

The objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty-six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 progenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in F5 and in approximately 90% of the susceptible individuals in F6. However, the polymorphic EST-SSR markers among populations contrasting for resistance to leaf miner were not correlated to the evaluated characteristics. SSR markers show inter- and intraspecific polymorphism in C. arabica and C. racemosa.

Sign in / Sign up

Export Citation Format

Share Document