scholarly journals Dietary calcium does not interact with vitamin D3 in terms of determining the response and catabolism of serum 25-hydroxyvitamin D during winter in older adults

2014 ◽  
Vol 99 (6) ◽  
pp. 1414-1423 ◽  
Author(s):  
Kevin D Cashman ◽  
Aoife Hayes ◽  
Sinead M O’Donovan ◽  
Joy Y Zhang ◽  
Michael Kinsella ◽  
...  
Keyword(s):  
Older Adults ◽  
Vitamin D3 ◽  
Dietary Calcium ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

scholarly journals Relative effectiveness of oral 25-hydroxyvitamin D3 and vitamin D3 in raising wintertime serum 25-hydroxyvitamin D in older adults

2012 ◽  
Vol 95 (6) ◽  
pp. 1350-1356 ◽  
Author(s):  
Kevin D Cashman ◽  
Kelly M Seamans ◽  
Alice J Lucey ◽  
Elisabeth Stöcklin ◽  
Peter Weber ◽  
...  
Keyword(s):  
Older Adults ◽  
Vitamin D3 ◽  
Relative Effectiveness ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

Associations of Dietary Calcium, Vitamin D, Milk Intakes, and 25-Hydroxyvitamin D With Bone Mass in Spanish Adolescents: The HELENA Study

2013 ◽  
Vol 16 (1) ◽  
pp. 110-117 ◽  
Author(s):  
Theodora Mouratidou ◽  
German Vicente-Rodriguez ◽  
Luis Gracia-Marco ◽  
Inge Huybrechts ◽  
Isabelle Sioen ◽  
...  
Keyword(s):  
Vitamin D ◽  
Bone Mass ◽  
Dietary Calcium ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Helena Study

Plasma 25-hydroxyvitamin D levels, quality of life, inflammation and depression in older adults: Are they related?

2021 ◽  
pp. 111503
Author(s):  
Ligia Pereira Martins Quessada ◽  
Carla Manuela Crispim Nascimento ◽  
Fabiana de Souza Orlandi ◽  
Aline Cristina Martins Gratão ◽  
Fernando Augusto Vasilceac ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Older Adults ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

scholarly journals Response of the 5′-flanking region of the human 25-hydroxyvitamin D 1α-hydroxylase gene to physiological stimuli using a transgenic mouse model

2005 ◽  
Vol 34 (1) ◽  
pp. 237-245 ◽  
Author(s):  
I Hendrix ◽  
P H Anderson ◽  
J L Omdahl ◽  
B K May ◽  
H A Morris
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Transgenic Mouse ◽  
Reporter Gene ◽  
Dietary Calcium ◽  
Mrna Levels ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Flanking Region

The enzyme 25-hydroxyvitamin D 1α-hydroxylase, or CYP27B1, is the key enzyme in the two-step activation process of vitamin D to 1,25-dihydroxyvitamin D (1,25D). While a number of regulators of the renal CYP27B1 enzyme activity have been recognized for some years, their underlying molecular mechanisms remain largely unknown, and the DNA regions involved in the in vivo regulation of gene expression by these factors have not been delineated. We have generated a transgenic mouse line that expresses 1501 bp of 5′ flanking region together with 44 bp of 5′ untranslated region of the human CYP27B1 gene fused to the firefly luciferase reporter gene. Animals expressing the luciferase gene demonstrated that both luciferase protein and mRNA for CYP27B1 were localized to proximal convoluted tubule cells of the kidney. In 2-week-old animals, the expression of the transgene and the endogenous CYP27B1 mRNA levels in the kidney were highest and fell with increasing age. Both reporter gene expression and CYP27B1 mRNA levels were downregulated in response to increasing amounts of dietary calcium in a dose-dependent manner. Vitamin D deficiency resulted in an increase in both the reporter gene and CYP27B1 expression. Interestingly, the increase in CYP27B1 mRNA levels was substantially higher than the increase in reporter gene expression, suggesting either that there is a post-transcriptional mechanism that increases the amount of CYP27B1 mRNA or that other regulatory elements are required to maximize the effect of vitamin D deficiency. These findings demonstrate that the 1501 bp 5′ flanking region of the CYP27B1 gene directs expression to the proximal convoluted tubules of the kidney and is responsible for increasing transcriptional activity when dietary calcium and vitamin D levels are depleted. It also responds in the kidney to the physiological regulators of development and ageing.

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