scholarly journals Human fetal kidney cells regenerate acellular porcine kidneys via upregulation of key transcription factors involved in kidney developmentRunning title: Regeneration of porcine kidneys

2019 ◽  
Vol 3 (1) ◽  
pp. 26-46
Author(s):  
Vijay Kumar Kuna ◽  
Sanchari Paul ◽  
◽  
Bo Xu ◽  
Robert Sjöback ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Kidney Development ◽  
Kidney Cells ◽  
Fetal Kidney ◽  
Human Fetal Kidney

scholarly journals Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development

PLoS Biology ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. e3000152 ◽  
Author(s):  
Mazène Hochane ◽  
Patrick R. van den Berg ◽  
Xueying Fan ◽  
Noémie Bérenger-Currias ◽  
Esmée Adegeest ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Kidney Development ◽  
Fetal Kidney ◽  
Gene Expression Dynamics ◽  
Human Fetal Kidney

Human Butyrylcholinesterase L330I Mutation Belongs to a Fluoride-Resistant Gene, by Expression in Human Fetal Kidney Cells

1997 ◽  
Vol 240 (2) ◽  
pp. 372-375 ◽  
Author(s):  
Kayoko Sudo ◽  
Masato Maekawa ◽  
Setsuko Akizuki ◽  
Tadao Magara ◽  
Hisataka Ogasawara ◽  
...  
Keyword(s):  
Kidney Cells ◽  
Fetal Kidney ◽  
Resistant Gene ◽  
Human Butyrylcholinesterase ◽  
Human Fetal Kidney

scholarly journals FP065THE AVERAGE AREAS OF GLOMERULAR PROFILES IN DIFFERENT CORTICAL ZONES DURING HUMAN FETAL KIDNEY DEVELOPMENT

2015 ◽  
Vol 30 (suppl_3) ◽  
pp. iii86-iii86
Author(s):  
Marija Bjelakovic ◽  
Slobodan Vlajkovic ◽  
Goran Bjelakovic
Keyword(s):  
Kidney Development ◽  
Fetal Kidney ◽  
Human Fetal Kidney

scholarly journals Dissecting the Global Dynamic Molecular Profiles of Human Fetal Kidney Development by Single-Cell RNA Sequencing

Cell Reports ◽  
2018 ◽  
Vol 24 (13) ◽  
pp. 3554-3567.e3 ◽  
Author(s):  
Ping Wang ◽  
Yidong Chen ◽  
Jun Yong ◽  
Yueli Cui ◽  
Rui Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Kidney Development ◽  
Fetal Kidney ◽  
Global Dynamic ◽  
Single Cell Rna Sequencing ◽  
Molecular Profiles ◽  
Human Fetal Kidney

Stereological study of developing glomerular forms during human fetal kidney development

2017 ◽  
Vol 33 (5) ◽  
pp. 817-825 ◽  
Author(s):  
Marija Dakovic Bjelakovic ◽  
Slobodan Vlajkovic ◽  
Aleksandar Petrovic ◽  
Marko Bjelakovic ◽  
Milorad Antic
Keyword(s):  
Kidney Development ◽  
Fetal Kidney ◽  
Human Fetal Kidney

scholarly journals Quantitative analysis of the nephron during human fetal kidney development

2005 ◽  
Vol 62 (4) ◽  
pp. 281-286 ◽  
Author(s):  
Marija Dakovic-Bjelakovic ◽  
Slobodan Vlajkovic ◽  
Rade Cukuranovic ◽  
Svetlana Antic ◽  
Goran Bjelakovic ◽  
...  
Keyword(s):  
Gestational Age ◽  
Kidney Development ◽  
Kidney Tissue ◽  
Human Kidney ◽  
Volume Density ◽  
Ureteric Bud ◽  
Fetal Kidney ◽  
Intrauterine Development ◽  
Human Fetal Kidney ◽  
Nephrogenic Zone

Background. The development of human kidney is a complex process. The number, shape, size, and distribution of nephrons as functional units in a kidney, provide some important information about the organization of the kidney. The aim of this study was to extend the knowledge of the developing human kidney by studying nephrons in the kidney's cortex during gestation. Methods. Kidney tissue specimens of 32 human fetuses, the gestational age from IV lunar month (LM IV) to LM X, were analyzed. Specimens were divided in ten groups based on gestational age. Stereological methods were used at the light microscopic level to estimate the volume densities of the corpuscular and tubular components of the nephron in the cortex of the developing human kidney. Results. Nephron polymorphism was the main characteristic of the human fetal kidney during development. In younger fetuses, just below the renal capsule, there was a wide nephrogenic zone. It contained the condensed mesenchyme and terminal ends of the ureteric bud. Nephrons, in the different stages of development, were located around the ureteric bud which branched in the cortical nephrogenic zone and induced nephrogenesis. More mature nephrons were located in the deeper part of the cortex, close to the juxta-medullary junction. During gestation, nephrogenesis continually advanced, and the number of nephrons increased. Glomeruli changed their size and shape, while the tubules changed their length and convolution. Renal cortex became wider and contained the more mature glomeruli and the more convoluted tubules. The volume density of the tubular component of the nephron increased continually from 10.53% (LM IVa) to 27.7% (LM X). Renal corpuscles changed their volume density irregularly during gestation, increasing from 13% (LM IVa) to 15.5% (LM IVb). During the increase of gestational age, the volume density of corpuscular component of the nephron decreased to 11.7% (LM VIII), then went on increasing until the end of the intrauterine development (LM X) when corpuscles occupied 16.73% of the cortical volume. The volume density of the developing nephrons (corpuscular and tubular portion) showed the significant positive correlation (r = 0.85; p<0.01) with gestational age. Conclusion. The present study was one of few quantitative studies of the human developing nephron. Knowledge about the normal development of the human kidney should be important for the future medical practice.

scholarly journals Possible Relevance of Soluble Luteinizing Hormone Receptor during Development and Adulthood in Boys and Men

Cancers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1329
Author(s):  
Li Juel Mortensen ◽  
Mette Lorenzen ◽  
Anne Jørgensen ◽  
Jakob Albrethsen ◽  
Niels Jørgensen ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Hormone Receptor ◽  
Adrenal Glands ◽  
Seminal Fluid ◽  
Body Fluids ◽  
Luteinizing Hormone Receptor ◽  
Gonadal Function ◽  
Fetal Kidney ◽  
Healthy Men ◽  
Human Fetal Kidney

Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are agonists for the luteinizing hormone receptor (LHCGR) which regulates male reproductive function. LHCGR may be released into body fluids. We wish to determine whether soluble LHCGR is a marker for gonadal function. Cross-sectional, longitudinal, and intervention studies on 195 healthy boys and men and 396 men with infertility, anorchia, or Klinefelter Syndrome (KS) were used to correlate LHCGR measured in serum, seminal fluid, urine, and hepatic/renal artery and vein with gonadal function. LHCGR was determined in fluids from in vitro and in vivo models of human testicular tissue and cell lines, xenograft mouse models, and human fetal kidney and adrenal glands. Western blot showed LHCGR fragments in serum and gonadal tissue of similar size using three different antibodies. The LHCGR-ELISA had no species cross-reactivity or unspecific reaction in mouse serum even after human xenografting. Instead, sLHCGR was released into the media after the culture of a human fetal kidney and adrenal glands. Serum sLHCGR decreased markedly during puberty in healthy boys (p = 0.0001). In healthy men, serum sLHCGR was inversely associated with the Inhibin B/FSH ratio (β −0.004, p = 0.027). In infertile men, seminal fluid sLHCGR was inversely associated with serum FSH (β 0.006, p = 0.009), sperm concentration (β −3.5, p = 0.003) and total sperm count (β −3.2, p = 0.007). The injection of hCG lowered sLHCGR in serum and urine of healthy men (p < 0.01). In conclusion, sLHCGR is released into body-fluids and linked with pubertal development and gonadal function. Circulating sLHCGR in anorchid men suggests that sLHCGR in serum may originate from and possibly exert actions in non-gonadal tissues. (ClinicalTrials: NTC01411527, NCT01304927, NCT03418896).

scholarly journals Placental Lactogen-I (PL-I) Target Tissues Identified with an Alkaline Phosphatase-PL-I Fusion Protein

1998 ◽  
Vol 46 (6) ◽  
pp. 737-743 ◽  
Author(s):  
Heiner Müller ◽  
Guoli Dai ◽  
Michael J. Soares
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Colorimetric Assay ◽  
Biological Activities ◽  
Placental Lactogen ◽  
Kidney Cells ◽  
Fetal Kidney ◽  
Transfected Cells ◽  
Tissue Sections ◽  
Labeling System

The rat placenta expresses a family of genes related to prolactin (PRL). Target tissues and physiological roles for many members of the PRL family have yet to be determined. In this investigation we evaluated the use of an alkaline phosphatase (AP) tag for monitoring the behavior of a prototypical member of the PRL family, placental lactogen-I (PL-I). A probe was generated consisting of a fusion protein of human placental AP and rat PL-I (AP-PL-I). The AP-PL-I construct was stably expressed in 293 human fetal kidney cells, as was the unmodified AP vector that served as a control. AP activity was monitored with a colorimetric assay in conditioned medium from transfected cells. Immunoreactivity and PRL-like biological activities of the AP-PL-I fusion protein were demonstrated by immunoblotting and the Nb2 lymphoma cell proliferation assay, respectively. AP-PL-I specifically bound to tissue sections known to express the PRL receptor, including the ovary, liver, and choroid plexus. Binding of AP-PL-I to tissues was specific and could be competed with ovine PRL. The results indicate that AP is an effective tag for monitoring the behavior of PL-I and suggest that this labeling system may also be useful for monitoring the actions of other members of the PRL family.

scholarly journals Expression of Stem Cell Markers in the Human Fetal Kidney

PLoS ONE ◽  
2009 ◽  
Vol 4 (8) ◽  
pp. e6709 ◽  
Author(s):  
Sally Metsuyanim ◽  
Orit Harari-Steinberg ◽  
Ella Buzhor ◽  
Dorit Omer ◽  
Naomi Pode-Shakked ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Markers ◽  
Fetal Kidney ◽  
Cell Markers ◽  
Human Fetal Kidney
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