Human Butyrylcholinesterase L330I Mutation Belongs to a Fluoride-Resistant Gene, by Expression in Human Fetal Kidney Cells

1997 ◽  
Vol 240 (2) ◽  
pp. 372-375 ◽  
Author(s):  
Kayoko Sudo ◽  
Masato Maekawa ◽  
Setsuko Akizuki ◽  
Tadao Magara ◽  
Hisataka Ogasawara ◽  
...  
Keyword(s):  
Kidney Cells ◽  
Fetal Kidney ◽  
Resistant Gene ◽  
Human Butyrylcholinesterase ◽  
Human Fetal Kidney

scholarly journals Possible Relevance of Soluble Luteinizing Hormone Receptor during Development and Adulthood in Boys and Men

Cancers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1329
Author(s):  
Li Juel Mortensen ◽  
Mette Lorenzen ◽  
Anne Jørgensen ◽  
Jakob Albrethsen ◽  
Niels Jørgensen ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Hormone Receptor ◽  
Adrenal Glands ◽  
Seminal Fluid ◽  
Body Fluids ◽  
Luteinizing Hormone Receptor ◽  
Gonadal Function ◽  
Fetal Kidney ◽  
Healthy Men ◽  
Human Fetal Kidney

Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are agonists for the luteinizing hormone receptor (LHCGR) which regulates male reproductive function. LHCGR may be released into body fluids. We wish to determine whether soluble LHCGR is a marker for gonadal function. Cross-sectional, longitudinal, and intervention studies on 195 healthy boys and men and 396 men with infertility, anorchia, or Klinefelter Syndrome (KS) were used to correlate LHCGR measured in serum, seminal fluid, urine, and hepatic/renal artery and vein with gonadal function. LHCGR was determined in fluids from in vitro and in vivo models of human testicular tissue and cell lines, xenograft mouse models, and human fetal kidney and adrenal glands. Western blot showed LHCGR fragments in serum and gonadal tissue of similar size using three different antibodies. The LHCGR-ELISA had no species cross-reactivity or unspecific reaction in mouse serum even after human xenografting. Instead, sLHCGR was released into the media after the culture of a human fetal kidney and adrenal glands. Serum sLHCGR decreased markedly during puberty in healthy boys (p = 0.0001). In healthy men, serum sLHCGR was inversely associated with the Inhibin B/FSH ratio (β −0.004, p = 0.027). In infertile men, seminal fluid sLHCGR was inversely associated with serum FSH (β 0.006, p = 0.009), sperm concentration (β −3.5, p = 0.003) and total sperm count (β −3.2, p = 0.007). The injection of hCG lowered sLHCGR in serum and urine of healthy men (p < 0.01). In conclusion, sLHCGR is released into body-fluids and linked with pubertal development and gonadal function. Circulating sLHCGR in anorchid men suggests that sLHCGR in serum may originate from and possibly exert actions in non-gonadal tissues. (ClinicalTrials: NTC01411527, NCT01304927, NCT03418896).

scholarly journals Placental Lactogen-I (PL-I) Target Tissues Identified with an Alkaline Phosphatase-PL-I Fusion Protein

1998 ◽  
Vol 46 (6) ◽  
pp. 737-743 ◽  
Author(s):  
Heiner Müller ◽  
Guoli Dai ◽  
Michael J. Soares
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Colorimetric Assay ◽  
Biological Activities ◽  
Placental Lactogen ◽  
Kidney Cells ◽  
Fetal Kidney ◽  
Transfected Cells ◽  
Tissue Sections ◽  
Labeling System

The rat placenta expresses a family of genes related to prolactin (PRL). Target tissues and physiological roles for many members of the PRL family have yet to be determined. In this investigation we evaluated the use of an alkaline phosphatase (AP) tag for monitoring the behavior of a prototypical member of the PRL family, placental lactogen-I (PL-I). A probe was generated consisting of a fusion protein of human placental AP and rat PL-I (AP-PL-I). The AP-PL-I construct was stably expressed in 293 human fetal kidney cells, as was the unmodified AP vector that served as a control. AP activity was monitored with a colorimetric assay in conditioned medium from transfected cells. Immunoreactivity and PRL-like biological activities of the AP-PL-I fusion protein were demonstrated by immunoblotting and the Nb2 lymphoma cell proliferation assay, respectively. AP-PL-I specifically bound to tissue sections known to express the PRL receptor, including the ovary, liver, and choroid plexus. Binding of AP-PL-I to tissues was specific and could be competed with ovine PRL. The results indicate that AP is an effective tag for monitoring the behavior of PL-I and suggest that this labeling system may also be useful for monitoring the actions of other members of the PRL family.

scholarly journals Expression of Stem Cell Markers in the Human Fetal Kidney

PLoS ONE ◽  
2009 ◽  
Vol 4 (8) ◽  
pp. e6709 ◽  
Author(s):  
Sally Metsuyanim ◽  
Orit Harari-Steinberg ◽  
Ella Buzhor ◽  
Dorit Omer ◽  
Naomi Pode-Shakked ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Markers ◽  
Fetal Kidney ◽  
Cell Markers ◽  
Human Fetal Kidney

scholarly journals Spatial relationship between expression of cytokeratin-19 and that of connexin-43 in human fetal kidney

2013 ◽  
Vol 46 (1) ◽  
pp. 32 ◽  
Author(s):  
Keisuke Hieda ◽  
Shogo Hayashi ◽  
Ji Hyun Kim ◽  
Gen Murakami ◽  
Baik Hwan Cho ◽  
...  
Keyword(s):  
Connexin 43 ◽  
Spatial Relationship ◽  
Cytokeratin 19 ◽  
Fetal Kidney ◽  
Human Fetal Kidney

Growth and development of the human fetal kidney

1986 ◽  
Vol 53 (2) ◽  
pp. 273-279 ◽  
Author(s):  
G. K. Mehrotra ◽  
G. Datta ◽  
K. L. Mukherjee
Keyword(s):  
Growth And Development ◽  
Fetal Kidney ◽  
Human Fetal Kidney

scholarly journals Expression of the Von Hippel-Lindau Tumor Suppressor Gene, VHL, in Human Fetal Kidney and During Mouse Embryogenesis

1995 ◽  
Vol 1 (4) ◽  
pp. 457-466 ◽  
Author(s):  
Patricia M. Kessler ◽  
Sandip P. Vasavada ◽  
Raymond R. Rackley ◽  
Thomas Stackhouse ◽  
Fuh-Mei Duh ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Suppressor Gene ◽  
Fetal Kidney ◽  
Mouse Embryogenesis ◽  
Von Hippel Lindau ◽  
Human Fetal Kidney

scholarly journals Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development

PLoS Biology ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. e3000152 ◽  
Author(s):  
Mazène Hochane ◽  
Patrick R. van den Berg ◽  
Xueying Fan ◽  
Noémie Bérenger-Currias ◽  
Esmée Adegeest ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Kidney Development ◽  
Fetal Kidney ◽  
Gene Expression Dynamics ◽  
Human Fetal Kidney
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