Antagonistic Potential of Endophytic Bacteria Against Phytophthora palmivora Causing Black Pod Rot Disease on Cacao (Theobroma cacao L.) In Indonesia

2020 ◽  
Vol 19 (1) ◽  
pp. 22-41 ◽  
Author(s):  
Masanto . ◽  
Arif Wibowo ◽  
Tri Joko ◽  
Siti Subandiyah ◽  
Koji Kageyama
Keyword(s):  
Theobroma Cacao ◽  
Endophytic Bacteria ◽  
Phytophthora Palmivora ◽  
Black Pod ◽  
Antagonistic Potential ◽  
Rot Disease ◽  
Theobroma Cacao L

scholarly journals The expression of pathogenicity-related genes in Phytophthora palmivora causing black pod rot disease on cacao (Theobroma cacao L.) in Indonesia

2021 ◽  
Vol 16 (1) ◽  
pp. 284-295
Author(s):  
Masanto Masanto ◽  
Arif Wibowo ◽  
Nur Fathurahman Ridwan ◽  
Widhi Dyah Sawitri ◽  
Koji Kageyama ◽  
...  
Keyword(s):  
Theobroma Cacao ◽  
Phytophthora Palmivora ◽  
Black Pod ◽  
Rot Disease ◽  
Theobroma Cacao L

scholarly journals Symbiotic and endophytic fungi as biocontrols against cocoa (Theobroma cacao L.) phytopathogens

2017 ◽  
Vol 43 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Raquel Amanda Villamizar-Gallardo ◽  
Oscar Orlando Ortíz-Rodriguez ◽  
Jhon Wilmer Escobar
Keyword(s):  
Theobroma Cacao ◽  
Trichoderma Viride ◽  
Phytophthora Palmivora ◽  
Mycotoxigenic Fungi ◽  
Black Pod ◽  
Antagonistic Potential ◽  
Native Strains ◽  
Biological Methods ◽  
Theobroma Cacao L

ABSTRACT Cocoa (Theobroma cacao L.) is a tropical tree, seriously affected by fungal diseases. To control several pathogens, biological methods are prescribed since they are friendly to the environment and easy to use. The main objective of this study was to assess the biocontrol effect of two native strains, Trichoderma viride and Botryosphaeria quercum, on phytopathogens such as Phytophthora palmivora and Moniliophtora roreri, causal agents of black pod and frosty pod rot diseases, respectively. In addition, biocontrolers were faced on potential mycotoxigenic fungi such as Aspergills flavus and Fusarium solani, which are very common on cocoa. The Bio-Control Index (BCI) was calculated to determine the in vitro biocontrol effect against the four phytopathogens. Results indicated that the best biocontrol agent of phytopathogens was B. quercum, showing BCI of 82.3%, 80.7%, 63.3% and 59.7% for each tested phytopathogen, respectively. Competition for substrate was the dominant biocontrol strategy. As to the origin of strains, those coming from the Department Norte de Santander and Santander showed the highest average inhibition percentage. This study provides an initial screening to the endophytic and antagonistic potential of fungi, specifically those capable of colonizing cocoa pods and soils. Thus, these strains can be used as an efficient biological control alternative against several known phytopathogens of cocoa in the field.

Genetic diversity of native cultivated cacao accessions (Theobroma cacao L.) in Nicaragua

2012 ◽  
Vol 10 (3) ◽  
pp. 254-257
Author(s):  
Erwin Aragon ◽  
Claudia Rivera ◽  
Helena Korpelainen ◽  
Aldo Rojas ◽  
Paula Elomaa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Theobroma Cacao ◽  
Phytophthora Palmivora ◽  
Genetic Composition ◽  
Breeding Programmes ◽  
Black Pod ◽  
Black Pod Disease ◽  
Parental Lines ◽  
Resistant Genotypes ◽  
Theobroma Cacao L

A total of 60 farmers' cacao accessions (Theobroma cacao L.) from Nicaragua were investigated using microsatellite markers to reveal their genetic composition and to identify potentially resistant genotypes against the black pod disease caused by Phytophthora palmivora. These accessions were compared with 21 breeders' accessions maintained locally, two Criollo accessions from Costa Rica and two accessions from Ecuador. The analyses showed a low level of differentiation among groups of farmers' accessions (FST = 0.06) and that six Nicaraguan accessions were genetically closely related to the two Criollo accessions used as a reference. In addition, seven distinct genotypes were found to have allelic composition that may indicate linkage to resistance alleles, thus being potential parental lines in future breeding programmes.

scholarly journals ​Control Activity and Antibiotic Gene Detection of Endophytic Bacteria in Suppressing Cocoa Black Pod Disease (Phytophthora palmivora Butl.)

2021 ◽  
Author(s):  
Rian Arini ◽  
Gusti Ayu Kade Sutariati ◽  
Andi Khaeruni ◽  
Teguh Wijayanto ◽  
Novita Pramahsari Putri ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Disease Suppression ◽  
Phytophthora Palmivora ◽  
Antibiotic Biosynthesis ◽  
Bacterial Isolates ◽  
Pcr Product ◽  
Black Pod ◽  
Black Pod Disease ◽  
Rot Disease

Background: Black pod rot disease of cocoa caused by (Phytophthora palmivora Butl.) is one of the major diseases on cocoa plantations worldwide. Many attempts have been made to prevent or reduce the infection of pathogens, but they have not provided optimum results. This study aims to detect antibiotic genes in endophytic bacteria that can suppress cocoa black pod disease. Methods: Eight endophytic bacteria were isolated from healthy cocoa pods and twigs that showed potentials in suppressing P. palmivora growth in vitro were used in suppressing of black pod rot disease in vivo tests. Antibiotic biosynthesis-related genes from eight endophytic bacterial isolates were confirmed by using PCR method, which includes phenazine-1-carboxylic (PCA), pyrrolnitrin (PRN), phenazine-1-carboxamide acid (PCN), pyoluteorin (PLT) and 2,4-diacetylphloroglucinol (DPAG/Phl). Result: The endophytic bacteria, 4RSI, 5BR B3 and 2RW B2 isolates showed the highest disease suppressing index to black pod rot disease in vivo, i.e., 70.27%, 70.08% and 56.64%. The isolates 5BR B3 and 2RW B2 DNA yielded PCR product by using PCA primers (1400 bp), PRN primers (700 bp) and DAPG primers (1600 bp), while the 5RSI isolate yield PCR product using PRN primers only. Endophytic bacterial isolates 5BRB3 and 2RW B2 provided partial disease suppression to pod rot disease by inhibit pathogen growth and antibiotic compounds production.

DNA-based electrochemical nanobiosensor for the detection of Phytophthora palmivora (Butler) Butler, causing black pod rot in cacao (Theobroma cacao L.) pods

2019 ◽  
Vol 107 ◽  
pp. 14-20
Author(s):  
Anthony James DM. Franco ◽  
Florinia E. Merca ◽  
Myrna S. Rodriguez ◽  
Johnny F. Balidion ◽  
Veronica P. Migo ◽  
...  
Keyword(s):  
Theobroma Cacao ◽  
Phytophthora Palmivora ◽  
Black Pod ◽  
Theobroma Cacao L

scholarly journals Phytophthora palmivora–Cocoa Interaction

2020 ◽  
Vol 6 (3) ◽  
pp. 167
Author(s):  
Francine Perrine-Walker
Keyword(s):  
Papua New Guinea ◽  
Plant Immunity ◽  
Model Systems ◽  
Phytophthora Palmivora ◽  
In Planta ◽  
Plant Host ◽  
Black Pod ◽  
Rot Disease ◽  
Theobroma Cacao L

Phytophthora palmivora (Butler) is an hemibiotrophic oomycete capable of infecting over 200 plant species including one of the most economically important crops, Theobroma cacao L. commonly known as cocoa. It infects many parts of the cocoa plant including the pods, causing black pod rot disease. This review will focus on P. palmivora’s ability to infect a plant host to cause disease. We highlight some current findings in other Phytophthora sp. plant model systems demonstrating how the germ tube, the appressorium and the haustorium enable the plant pathogen to penetrate a plant cell and how they contribute to the disease development in planta. This review explores the molecular exchange between the oomycete and the plant host, and the role of plant immunity during the development of such structures, to understand the infection of cocoa pods by P. palmivora isolates from Papua New Guinea.

Isolasi dan Identifikasi Cendawan Filoplan yang Berpotensi Sebagai Agensia Pengendali Cendawan Patogen Phytopthora palmivora pada Tanaman Kakao

Agrotek ◽  
2018 ◽  
Vol 2 (4) ◽  
Author(s):  
Paulus Woyen
Keyword(s):  
Human Health ◽  
Theobroma Cacao ◽  
Phytophthora Palmivora ◽  
Isolation And Identification ◽  
Rot Disease ◽  
Theobroma Cacao L

Cocoa (Theobroma cacao L.) plantations �development in Indonesia have been made effort today almost in every areas, but not improved yield. An inhibitor factor is pest and disease invasion. Controling of cocoa pod rot disease caused by Phytophthora palmivora in common suggestion is used of sintetic fingicide, but it has hazard effect to environment, animals and human health, thus today, friendly controling tecnology by used of antagonistic microorganism is being developed. Common Antagonistic microorganism used were soil-inhabitant (rhizosphere). It supposed that, there were plant�s surface-inhahabitant (phyloplen) fungi which potentially as antagonist agent to P. palmivora. This research was obtained to isolation and identification of ph

PENGAMATAN GEJALA INFEKSI Phytophthora palmivora PENYEBAB PENYAKIT BUSUK BUAH PADA KAKAO

Biocelebes ◽  
2019 ◽  
Vol 13 (3) ◽  
pp. 253-261
Author(s):  
Nurfianti ◽  
Umrah
Keyword(s):  
Active Ingredient ◽  
Theobroma Cacao ◽  
Phytophthora Palmivora ◽  
The Third ◽  
Time Of Infection ◽  
Rot Disease ◽  
Aspergillus Sp ◽  
Central Sulawesi ◽  
Theobroma Cacao L

Phytophthora palmivora is a patogen fungi causing pod rot disease in cacao plants. Thr researchof effectivity examination of biopesticide (active ingredient Aspergillus sp.) againts the growth of P. Palmivora in  cacao pod (Theobroma cacao L.) by in vivo state was conducted from March 2018 to September 2018. This research was carried out in Sidondo Village, Dolo Subdistrict, Sigi Biromaru District, Central Sulawesi Province and Laboratory of Biotechnology, Faculity of Mathematics and Natural Sciences, Tadulako University. This study was aimed to determine an effective biopesticide (active ingredient Aspergillus sp.) which suppresses the growth of P. Palmivora in  cacao pod by in vivo state and to stipulate the most affective  biopesticide concentration (active ingredient Aspergillus sp.) in suppressing the growth of of P. Palmivora in  cacao pod by in vivo state. This This research was by using the methods of Group Random Design  (GRD) or Rancangan Acak Kelompok (RAK) consisting of six treatmentsand four repetitions. The observation parameters consist of symptoms of disease, the lenght of infection spots, the infection persentage of P. Palmivora, microscopic observations, in the microscopic of P. Palmivora. The results showed that the biopesticide (active ingredient Aspergillus sp.) was varified as effective in controlling P. Palmivora drawing the rot disease bin cacao plants, by the absent of any symptoms of infection P1,P2,P3,P4 dan P5 treatments. The only treatments in which was infected by P. Palmivora , drawing the rot disease in cacao plants, was P0 with the occurence time of infection symptoms was in the third day after inoculation, the average lenght of infection spots was 8,65 cm and the average infection persentage was 11,60%.

scholarly journals PENDUGAAN DAYA GABUNG DAN HETEROSIS KETAHANAN TANAMAN KAKAO (Theobroma cacao L.) TERHADAP PENYAKIT BUSUK BUAH (Phytophthora palmivora)

2020 ◽  
Vol 17 (3) ◽  
pp. 124
Author(s):  
RUBIYO RUBIYO ◽  
TRIKOESOEMANINGTYAS TRIKOESOEMANINGTYAS ◽  
SUDARSONO SUDARSONO
Keyword(s):  
Combining Ability ◽  
Theobroma Cacao ◽  
Genetic Parameters ◽  
General Combining Ability ◽  
Phytophthora Palmivora ◽  
F1 Hybrid ◽  
Plastic Bag ◽  
Black Pod ◽  
Black Pod Disease ◽  
Theobroma Cacao L

<p>ABSTRAK</p><p>Klon kakao unggul berdaya hasil dan bermutu hasil tinggi sertaresisten terhadap penyakit utama perlu dikembangkan melalui pemuliaantanaman dan tersedianya informasi tentang parameter genetik diharapkandapat membantu memecahkan masalah tersebut. Pendugaan parametergenetik dapat dilakukan dengan analisis persilang dialel. Penelitianbertujuan untuk menduga parameter genetik ketahanan tanaman kakaoterhadap infeksi P. palmivora, menggunakan analisis persilangan setengahdialel. Bahan tanaman terdiri atas lima klon kakao (ICCRI 3, TSH 858,DR 1, ICS 13 dan Sca 6) yang tergolong rentan hingga tahan terhadapinfeksi P. palmivora yang digunakan sebagai tetua dan 10 galur hibrida F1hasil persilangan antar lima klon. Penelitian dilaksanakan di KebunPercobaan Kaliwining Pusat Penelitian Kopi dan Kakao Indonesia,Jember, Jawa Timur dari tahun 2008 hingga 2009. Untuk setiap kombinasipersilangan dievaluasi 20 bibit dan diulang tiga kali. Untuk mengetahuirespon bibit hibrida F1 terhadap infeksi P. palmivora, daunnya diinokulasidengan inokulum zoospora dan disungkup dengan plastik untuk menjagakelembapannya (&gt;90%). Pengamatan luas bercak akibat infeksi P.palmivora dilakukan enam hari setelah inokulasi dan digunakan untukmenghitung intensitas penyakit. Hasil penelitian menunjukkan bahwakakao klon DR 1, ICS 13, dan ICCRI 3 mempunyai DGU yang palingtinggi dibandingkan dengan tetua lainnya. Selanjutnya, persilangan antarklon kakao DR 1 x ICS 13, dan TSH 858 x Sca 6 mempunyai DGKtertinggi sehingga kombinasi persilangan ini berpeluang untuk menjadipenghasil hibrida baru yang resisten terhadap P. palmivora. Kombinasipersilangan yang menunjukkan nilai heterosis tertinggi adalah DR1 x ICS13, DR1 x Sca 6, dan ICS 13 x Sca 6.</p><p>Kata kunci: Heterosis, hibrida F1, DGU, DGK, intensitas penyakit</p><p>ABSTRACT</p><p>Estimation of Heterosis and Combining Ability forResistance Against Black Pod Disease (Phytophthorapalmivora) in Cacao (Theobroma cacao L.)</p><p>High yielding and disease resistance of cacao clone needs to bedeveloped through breeding program. Availability of genetic parametersfor various agronomic importance characters in cacao would be veryuseful and beneficial for cacao breeding activities. Estimation of variousgenetic parameters could be done by analyzing F1 arrays generated fromsemi-diallele crosses among a number of parents. The objectives of thisresearch were to estimate genetic parameters for resistance against P.palmivora infection in cacao using F1 arrays generated from semi-diallelecrosses among five cacao clones. Five cacao clones (DR 1, TSH 858, ICS13, ICCRI 3, and Sca 6), representing an arrays of clones with increasedresistance against P. palmivora infection, were used as parents to generate10 F1 hybrid arrays. This research was conducted at KaliwiningExperimental Station, Indonesian Coffee and Cacao Research Institute,Jember, Indonesia, during the period of 2008 to 2009. At most 20seedlings were evaluated for each F1 hybrid and the evaluation wasreplicated three times. To evaluate the response of the seedlings against P.palmivora infection, their leaves were inoculated with zoospore of P.palmivora. Relative humidity around inoculated leaves was maintained at&gt;90% by wrapping them with plastic bag. The sizes of leaf necroseresponse due to P. palmivora infection were observed during six days afterinoculation and the disease intensity was calculated based on this recordedsymptoms. Results of the experiments indicated that cacao clones (DR 1,ICS 13, and ICCRI 3) were the highest in general combining ability(GCA) for resistance character than the other two clones. Moreover, F1hybrid originated from crosses between DR 1 x ICS 13, and TSH 858 xSca 6 were the highest in specific combining ability (SCA) for resistancecharacter. Therefore, this combination crosses might be used to developenew hybrid combinations resistance against P. palmivora infection.Combination crosses showing highest heterotic value for resistance againstP. palmivora infection were DR 1 x ICS 13, DR1 x Sca 6, and ICS 13 xSca 6.</p><p>Key words : Heterotic effects, F1 hybrid array, GCA, SCA, diseaseintensity</p>

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