Effect of Combined Use of Bacillus subtilis CA32 and Trichoderma harzianum RU01 on Biological Control of Rhizoctonia solani on Solanum melongena and Capsicum annuum

2009 ◽  
Vol 8 (1) ◽  
pp. 9-16 ◽  
Author(s):  
S. Abeysinghe
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Rhizoctonia Solani ◽  
Capsicum Annuum ◽  
Trichoderma Harzianum ◽  
Solanum Melongena ◽  
Combined Use

scholarly journals Application of plant extracts and Trichoderma harzianum for the management of tomato seedling damping-off caused by Rhizoctonia solani

2020 ◽  
Vol 116 (11/12) ◽  
Author(s):  
Mapula T.P. Hlokwe ◽  
Mapotso Kena ◽  
David N. Mamphiswana
Keyword(s):  
Biological Control ◽  
Rhizoctonia Solani ◽  
Plant Extracts ◽  
Trichoderma Harzianum ◽  
Moringa Oleifera ◽  
Plant Diseases ◽  
Yield Reduction ◽  
Biotic Factors ◽  
Damping Off ◽  
Tomato Seedling

Seedling production under smallholder farming systems can be negatively affected by both abiotic and biotic factors. Seedling damping-off caused by Rhizoctonia solani is one of the major biotic factors which causes significant yield reduction. Management is mainly based on the application of synthetic fungicides and cultural practices. However, both methods have limitations which result in their inefficiency. Several studies have reported on the use of plant extracts and biological control to manage plant diseases. The aim of this study was to formulate an effective and practical approach to manage tomato seedling dampingoff using extracts of Monsonia burkeana and Moringa oleifera and a biological control agent Trichoderma harzianum. The efficacy of both extracts was investigated under laboratory conditions to determine the most suppressive concentration to R. solani growth. Methanolic extracts from both plants significantly suppressed pathogen growth at different concentrations. M. burkeana significantly reduced R. solani growth at 8 g/mL (71%) relative to control whilst Moringa oleifera extract reduced pathogen growth by 60% at a concentration of 6 g/mL. The highest suppressive concentrations were further evaluated under greenhouse conditions to test their efficacy on seedling damping-off. In damping-off treatments, both plant extracts and T. harzianum also significantly reduced (p=0.5) pre- and post-emergence dampingoff incidence. M. burkeana recorded the highest suppression at 78%, followed by M. oleifera at 64%. Trichoderma harzianum reduced incidence of damping-off by 60% and this was higher than both plant extract treatments.

scholarly journals Efficacy of Bacillus subtilis V26 as a biological control agent against Rhizoctonia solani on potato

2015 ◽  
Vol 338 (12) ◽  
pp. 784-792 ◽  
Author(s):  
Saoussen Ben Khedher ◽  
Olfa Kilani-Feki ◽  
Mouna Dammak ◽  
Hayfa Jabnoun-Khiareddine ◽  
Mejda Daami-Remadi ◽  
...  
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Rhizoctonia Solani ◽  
Biological Control Agent ◽  
Control Agent

scholarly journals RhizosphericBacillus subtilisExhibits Biocontrol Effect againstRhizoctonia solaniin Pepper(Capsicum annuum)

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yuanyuan Huang ◽  
Zhansheng Wu ◽  
Yanhui He ◽  
Bang-Ce Ye ◽  
Chun Li
Keyword(s):  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Capsicum Annuum ◽  
Mycelial Growth ◽  
Biocontrol Agent ◽  
Growth Strain ◽  
Inhibition Rate ◽  
Pepper Plants

This study aimed at evaluating the ability of SL-44 to controlRhizoctonia solaniand promote pepper(Capsicum annuum)growth. Strain SL-44 was isolated from plant rhizosphere and the pot experiment results indicated that the dry and fresh weights of pepper in SL-44 andRhizoctonia solani(S-R) treatment were 45.5% and 54.2% higher than those inRhizoctonia solani(R) treatment and 18.2% and 31.8% higher than those in CK (control, noninoculation) treatment. The plant height in S-R treatment increased by 14.2% and 9.0% compared with those in the R and CK treatments, respectively. In vitro antagonism assay showed that SL-44 exhibited strong antifungal activity against the mycelial growth ofRhizoctonia solani, with an inhibition rate of 42.3%. The amount of phosphorus dissolved by SL-44 reached 60.58 mg·L−1in broth and 7.5 μg·mL−1IAA were secreted by SL-44. Strain SL-44 inhibited the growth ofR. solaniand improved biomass of pepper plants. Mass exchange and information transmission between the pepper plants and SL-44 mutually promoted their development.Bacillus subtilisSL-44 has a great potential as biocontrol agent againstRhizoctonia solanion pepper plants.

scholarly journals Biological control of Rhizoctonia solani in tomatoes with Trichoderma harzianum mutants

2010 ◽  
Vol 13 (2) ◽  
Author(s):  
Jaime Montealegre ◽  
Luis Valderrama ◽  
Soledad Sánchez ◽  
Rodrigo Herrera ◽  
Ximena Besoain ◽  
...  
Keyword(s):  
Biological Control ◽  
Rhizoctonia Solani ◽  
Trichoderma Harzianum

scholarly journals Biological Control of the Artillery Fungus, Sphaerobolus stellatus, with Trichoderma harzianum and Bacillus subtilis

2001 ◽  
Vol 19 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Elizabeth A. Brantley ◽  
Donald D. Davis ◽  
Larry J. Kuhns
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Trichoderma Harzianum ◽  
Biocontrol Agent ◽  
Biocontrol Agents ◽  
Biological Control Agents ◽  
Oatmeal Agar ◽  
Time Of Application ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

Abstract Three strains of the fungus Trichoderma harzianum Rifai and two strains of the bacterium Bacillus subtilis (Ehrenberg) Cohn were evaluated for their ability to suppress colonization and sporulation of the artillery fungus (Sphaerobolus stellatus Tode:Pers.) on oatmeal agar. All five biological control agents inhibited growth of S. stellatus, but efficacy depended on time of application. Simultaneous inoculation of agar with S. stellatus and the biocontrol agents, as well as inoculation of biocontrol agents 14 days prior to S. stellatus, resulted in complete inhibition of S. stellatus. Inoculation of agar with biocontrol agents 14 days after inoculation with S. stellatus reduced, but did not completely suppress S. stellatus colonization and sporulation. In this experiment, gleba (spore masses) treated with all strains of T. harzianum and strain GBO3 of B. subtilis did not germinate, but 13% of gleba treated with strain MBI 600 of B. subtilis did germinate. Trichoderma harzianum was more effective than B. subtilis as a biocontrol agent.

scholarly journals Gljive i bakterije u biološkoj kontroli uzročnika bolesti biljaka

2019 ◽  
Vol 42 (4) ◽  
pp. 38-43
Author(s):  
Magdalena Matić ◽  
Tamara Siber
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Thuringiensis ◽  
Rhizoctonia Solani ◽  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Trichoderma Viride ◽  
Helminthosporium Solani ◽  
Trichoderma Koningii

Zbog stalnog povećanja upotrebe kemijskih pesticida i njihovog štetnog djelovanja na ljudsko zdravlje i okoliš, sve veće zanimanje pobuđuju metode biološke kontrole uzročnika biljnih bolesti. Biološka kontrola je mjera koja podrazumijeva korištenje mikroorganizama koji smanjuju populaciju štetnika i patogena i na taj način štite biljku i kontroliraju razvoj bolesti. U Hrvatskoj je trenutno na tržištu prisutan samo jedan biofungicid, na bazi bakterije iz roda Pseudomonas, za suzbijanje uzročnika bijele noge (Rhizoctonia solani) na krumpiru, batatu, čičoki i kineskom jamu te uzročnika srebrolikosti gomolja (Helminthosporium solani) na krumpiru. Vrste Bacillus thuringiensis, Bacillus subtilis i Pseudomonas fluorescens su najvažniji predstavnici bakterija uključenih u biokontrolu protiv uzročnika biljnih bolesti. Do danas je na svjetskom tržištu prisutno ili je u procesu registracije 28 sredstava na bazi različitih vrsta roda Trichoderma, koji većinom djeluju protiv fitopatogenih gljiva iz rodova Pythium, Rhizoctonia i Fusarium. Najzastupljenije vrste roda Trichoderma koje se koriste u biološkoj kontroli su Trichoderma harzianum, Trichoderma viride i Trichoderma koningii. Upotreba antagonističkih gljiva i bakterija u biološkoj kontroli brojnih uzročnika bolesti predstavlja važnu alternativu kojom bi se mogli zamijeniti kemijski pesticidi ili u što većoj mjeri smanjiti njihova primjena. Cilj rada je prikazati antagonističke gljive i bakterije koje se koriste u biološkoj kontroli te opisati njihove složene mehanizme djelovanja.

The effect of inoculation of seed with antagonists of Rhizoctonia solani on the growth of wheat

1974 ◽  
Vol 25 (2) ◽  
pp. 213 ◽  
Author(s):  
PR Merriman ◽  
RD Price ◽  
KF Baker
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Grain Yield ◽  
Rhizoctonia Solani ◽  
Dry Matter ◽  
Streptomyces Griseus ◽  
Growth Stimulation ◽  
Seed Inoculation ◽  
Field Soils

Biological control of Rhizoctonia solani on wheat by seed inoculation with microorganisms was investigated. Initially inoculants were selected from bacteria and actinomycetes isolated from two soils and screened for antagonism to R. solani on agar. Of 148 isolates tested on agar, 96 were antagonistic to R. solani and of these 42 were added to pasteurized soil sown with peppers and inoculated with the pathogen. Seven isolates which controlled disease were screened further as seed inoculants of wheat in pasteurized and field soils inoculated with R. solani. Four inoculants, viz. Streptomyces griseus 2-A24 and three Bacillus subtilis isolates 1-B80, 1-B77 and 1-B68, significantly reduced symptoms caused by R. solani and also increased grain yield and dry matter of wheat. S. griseus 2-A24 and B. subtilis 1-B80 increased grain yield by 30% over controls. The magnitude of these differences suggests that biological control and growth stimulation are involved in yield increases.

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