Attempts to Improve the Method for Screening Cowpea Germplasm for Resistance to Cucumber Mosaic Virus and Blackeye Cowpea Mosaic Virus

2007 ◽  
Vol 6 (2) ◽  
pp. 202-205 ◽  
Author(s):  
A.G. Gillaspie Jr.
HortScience ◽  
1996 ◽  
Vol 31 (5) ◽  
pp. 762b-762
Author(s):  
A.G. Hunter ◽  
G.E. Boyhan ◽  
E.H. Simonne ◽  
O.L. Chambliss

Seed harvested from 41 entries in the 1994 southernpea variety trial was grown in a greenhouse for evaluation of seedborne mosaic viruses. When second trifoliate leaves were fully expanded, 100 plants per plot per block (4) were evaluated for blackeye cowpea mosaic virus (B1CMV), cucumber mosaic virus (CMV), cowpea severe mosaic virus (CSMV), and southern bean mosaic virus (SBMV). The average number of plants with virus symptoms ranged from 2% (Pinkeye Pinkpod) to 44% (Bettergreen). Plants with symptoms were assayed using enzyme-linked immunosorbent assay (ELISA). At least one virus was detected with ELISA in all entries, except for `Zipper Cream' in which none were evident. All viruses were detected in seven entries. B1CMV and CMV were present in 13. CMV was present in all but `Zipper Cream', `Mississippi Cream', and `Texas Pinkeye'. Symptomatology was poorly correlated to ELISA results: six entries having all four viruses had symptoms on less than 13% of their plants.


Agrosearch ◽  
2017 ◽  
Vol 17 (1) ◽  
pp. 11
Author(s):  
A.D. Ibrahim ◽  
M.T. Salaudeen ◽  
L.Y. Bello ◽  
A.A. Abdullahi ◽  
A.S. Adamu ◽  
...  

2004 ◽  
Vol 94 (1) ◽  
pp. 111-119 ◽  
Author(s):  
E. M. Martin ◽  
J. D. Cho ◽  
J. S. Kim ◽  
S. C. Goeke ◽  
K. S. Kim ◽  
...  

When two unrelated plant viruses infect a plant simultaneously, synergistic viral interactions often occur resulting in devastating diseases. This study was initiated to examine ultrastructural virus-virus interactions of mixed viral infections. Mixed infections were induced using potyviruses and viruses from other plant virus families. Novel ultrastructural paracrystalline arrays composed of co-infecting viruses, referred to as mixed virus particle aggregates (MVPAs), were noted in the majority of the mixed infections studied. When the flexuous rod-shaped potyvirus particles involved in MVPAs were sectioned transversely, specific geometrical patterns were noted within some doubly infected cells. Although similar geometrical patterns were associated with MVPAs of various virus combinations, unique characteristics within patterns were consistent in each mixed infection virus pair. Centrally located virus particles within some MVPAs appeared swollen (Southern bean mosaic virus mixed with Blackeye cowpea mosaic virus, Cucumber mosaic virus mixed with Blackeye cowpea mosaic virus, and Sunn hemp mosaic virus mixed with Soybean mosaic virus). This ultrastructural study complements molecular studies of mixed infections of plant viruses by adding the additional dimension of visualizing the interactions between the coinfecting viruses.


Sign in / Sign up

Export Citation Format

Share Document