Purification, Serology, and In Vitro Translation of an Alyce-clover Isolate of Blackeye Cowpea Mosaic Virus

Plant Disease ◽  
1991 ◽  
Vol 75 (3) ◽  
pp. 254 ◽  
Author(s):  
G. S. Zhao
Keyword(s):  
Mosaic Virus ◽  
In Vitro Translation ◽  
Cowpea Mosaic Virus ◽  
Blackeye Cowpea Mosaic Virus

cDNA cloning and in vitro transcription of the complete brome mosaic virus genome

1984 ◽  
Vol 4 (12) ◽  
pp. 2876-2882 ◽  
Author(s):  
P Ahlquist ◽  
M Janda
Keyword(s):  
Mosaic Virus ◽  
Transcription Initiation ◽  
Direct Sequencing ◽  
In Vitro Transcription ◽  
Brome Mosaic Virus ◽  
Viral Rna ◽  
In Vitro Translation ◽  
In Vitro Production ◽  
Genomic Rnas

Complete cDNA copies of each of the brome mosaic virus genomic RNAs (3.2, 2.8, and 2.1 kilobases in length) were cloned in a novel transcription vector, pPM1, designed to provide exact control of the transcription initiation site. After cleavage at a unique EcoRI site immediately downstream of the inserted cDNA, these clones can be transcribed in vitro by Escherichia coli RNA polymerase to yield complete copies of the brome mosaic virus RNAs. Dideoxy sequencing of 5' transcript cDNA runoff products and direct sequencing of 32P-3'-end-labeled transcripts show that such transcripts initiate at the same 5' position as natural viral RNA and terminate within the EcoRI runoff site after copying the entire viral RNA sequence. When synthesized in the presence of m7GpppG, the transcripts bear the natural capped 5' terminus of brome mosaic virus RNAs. Such transcripts direct the in vitro translation of proteins which coelectrophorese with the translation products of natural brome mosaic virus RNAs. pPM1 should facilitate in vitro production of other viral and nonviral RNAs.

In Vivo and in Vitro Translation of the RNAS of Alfalfa Mosaic Virus

1977 ◽  
pp. 387-411 ◽  
Author(s):  
L. van Vloten-Doting ◽  
J. Bol ◽  
L. Neeleman ◽  
T. Rutgers ◽  
D. van Dalen ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
In Vitro Translation

A comparative study on the in vitro translation products of individual RNAs from two-, three-, and four-component strains of barley stripe mosaic virus

Virology ◽  
1983 ◽  
Vol 127 (1) ◽  
pp. 1-14 ◽  
Author(s):  
V.V. Dolja ◽  
N.A. Lunina ◽  
R.-M. Leiser ◽  
T. Stanarius ◽  
S.N. Belzhelarskaya ◽  
...  
Keyword(s):  
Comparative Study ◽  
Mosaic Virus ◽  
In Vitro Translation ◽  
Barley Stripe Mosaic Virus

Ribosomes are stalled during in vitro translation of alfalfa mosaic virus RNA 1

1985 ◽  
Vol 152 (3) ◽  
pp. 625-631 ◽  
Author(s):  
Pim LINDHOUT ◽  
Lyda NEELEMAN ◽  
Hans TOL ◽  
Lous VLOTEN-DOTING
Keyword(s):  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
In Vitro Translation ◽  
Virus Rna
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