Effects of Crude Methanol Extract of Euphorbia hirta on Hematological and Biochemical Indices and Histological Changes of Liver in African Catfish Clarias gariepinus (Burchell, 1822)

2011 ◽  
Vol 6 (7) ◽  
pp. 802-808 ◽  
Author(s):  
A. Sheikhlar ◽  
A.R. Alimon ◽  
H. Daud ◽  
C.R. Saad ◽  
E. Ramezani- Fard
Keyword(s):  
Methanol Extract ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Histological Changes ◽  
Euphorbia Hirta ◽  
Biochemical Indices ◽  
Crude Methanol Extract

scholarly journals Histological Alterations in Some Organs of African Catfish (Clarias gariepinus) Exposed to Sub-lethal Concentrations of Glyphosate [N-(phosphonomethyl) glycine]

2021 ◽  
Vol 11 (2) ◽  
pp. 62-75
Author(s):  
Adedamola Akinbadewa ◽  
Henry Adewole ◽  
Stephen Adeyeni ◽  
Victor Olaleye
Keyword(s):  
Inflammatory Responses ◽  
Clarias Gariepinus ◽  
Life Forms ◽  
Average Weight ◽  
African Catfish ◽  
Aquatic Life ◽  
Histological Changes ◽  
Lc50 Value ◽  
Mucosal Secretions ◽  
Sublethal Concentrations

This study used a static bioassay to investigate the histological effects of glyphosate on the gill, liver and muscle of African catfish (Clarias gariepinus) fingerlings. This was done with a view of further characterising the effect of glyphosate on C. gariepinus fingerlings and other aquatic life forms. Six-week old C. gariepinus fingerlings with an average weight of 10.02 ± 0.2 g were stocked into three exposure sets (control, 2.75 ppm (25% of the 96 h LC50 value) and 5.50 ppm (50% of the 96 h LC50 value)) in triplicate at 30 fish per tank for 70 days. The 96 h LC50  value was 11.00 mg/L. Histological examination of the C. gariepinus exposed to various sublethal concentrations of glyphosate showed that major histological changes in their organs were concentration dependent such as gill arch vacuolation, excessive mucosal secretions, lifting of epithelial, and epithelium thickening, hyperplasia and telangiectasis in the gills, discolouration, change in form and consistent alterations involving hyperplasia, narrowing of the central nerve, necrosis, pkynosis, blood congestion and vacuolation of the liver, mild hyperplasia and inflammatory responses in the muscle of the fish. The severity of histological alteration was more pronounced in fish organs exposed to 5.50 ppm of glyphosate concentration. This study concluded that the toxicant (glyphosate) is highly toxic to C. gariepinus particularly at a concentration of 5.50 ppm, therefore its use near farm lands or adjacent water bodies should be discouraged.

Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WM Shaik Mossadeq ◽  
K Syamimi ◽  
MP Azyyati ◽  
ZA Zakaria ◽  
AK Arifah ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Methanol Extract ◽  
Guinea Pig Ileum ◽  
Mitragyna Speciosa ◽  
Anti Inflammatory ◽  
Crude Methanol Extract ◽  
Inflammatory Effect

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

PEMBEKUAN SEMEN LELE DUMBO (Clarias Gariepinus BURCHELL 1822) SEBAGAI MODEL KRIOPRESERVASI SEMEN IKAN

2010 ◽  
Vol 9 (1) ◽  
Author(s):  
Lutfi Lutfi
Keyword(s):  
Liquid Nitrogen ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Nitrogen Vapor ◽  
Dmso Concentration ◽  
Freezing Temperatures ◽  
Vapor Liquid

<p><em>Effect of four types of diluents</em><em>s</em><em> and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. </em><em>The steps in preparation the 16 treatments combination of the diluents</em><em> </em><em>are </em><em>preparation of diluents, mixing </em><em>diluents </em><em>with DMSO, packing </em><em>of semen </em><em>in</em><em>to</em><em> 0.3-ml straw, equilibration of </em><em>semen </em><em>at 4 °C for 30 minutes, freezing </em><em>of </em><em>semen in nitrogen vapor liquid </em><em>at </em><em>a height of 6.5 cm for 10 minutes</em><em>,</em><em> and subsequent</em><em>ly</em><em> storage of </em><em>semen </em><em>in liquid nitrogen (-196</em><em> <sup>0</sup></em><em>C) for further analy</em><em>sis</em><em> </em><em>of </em><em>post-thawing motility (PTM). </em><em>The result showed that t</em><em>he highest level of motility of spermatozoa </em><em>was </em><em>in </em><em>treatment </em><em>P</em><em><sub>1</sub></em><em>D</em><em><sub>15 </sub></em><em>(45.7 ± 4.3%) and the lowest </em><em>was </em><em>in </em><em>treatment </em><em>P<sub>2</sub>D<sub>20</sub> (14.5 ± 13.2%). The best diluent </em><em>in </em><em>this observation </em><em>was </em><em>diluents containing NaCl, KCl, CaCl<sub>2</sub> and NaHCO<sub>3</sub>. The best concentration </em><em>was </em><em>DMSO 15%. While the best interaction between the concentration of DMSO diluents is P<sub>1</sub>D<sub>15</sub> treatments containing NaCl, KCl, CaCl <sub>2</sub> and NaHCO<sub>3</sub> with a combination of 15% DMSO concentration. </em><em>The </em><em>conclusion </em><em>of the research is that </em><em>diluents containing NaCl, KCl, CaCl<sub>2</sub> and NaHCO<sub>3</sub> with a combination of 15% DMSO concentration</em><em> can be used in </em><em>cryopreservation of African catfish semen</em><em>.</em></p>

Vitamin E as antioxidant in female african catfish (Clarias gariepinus) exposed to chronic toxicity of atrazine

2012 ◽  
Vol 16 (2) ◽  
pp. 83-98 ◽  
Author(s):  
Shadia Kadry ◽  
Afnan Amer ◽  
Mohamed Marzouk ◽  
Magdy Hanna ◽  
Amal Azmy ◽  
...  
Keyword(s):  
Vitamin E ◽  
Chronic Toxicity ◽  
Clarias Gariepinus ◽  
African Catfish
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