Propagation of Infectious Bursal Disease Virus (IBDV) in Chicken Embryo Fibroblast Cells

2005 ◽  
Vol 6 (1) ◽  
pp. 146-149 ◽  
Author(s):  
SK. Amir Hossain . ◽  
Sarder Nasir Uddin . ◽  
Muhammad Sadiqur Rah . ◽  
Abdul Wadud . ◽  
Muhammad Harunuzzama .
Keyword(s):  
Disease Virus ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Infectious Bursal Disease ◽  
Fibroblast Cells ◽  
Bursal Disease

scholarly journals Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain in Ethiopia 

2021 ◽  
Author(s):  
Wakjira Kebebe ◽  
Molalegne Bitew ◽  
Fufa Dawo ◽  
Bedaso Mammo ◽  
Hawa Mohammed ◽  
...  
Keyword(s):  
Vero Cell ◽  
Disease Virus ◽  
Vaccine Strain ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Vero Cells ◽  
Infectious Bursal Disease ◽  
Efficacy Evaluation ◽  
Viral Pathogen ◽  
Bursal Disease

Abstract Background: Infectious bursal disease virus (IBDV) is an avian viral pathogen that causes infectious bursal disease (IBD) of chickens. The disease is endemic in Ethiopia since 2002 and vaccination is the major means of disease prevention and control. IBD vaccine is produced in Ethiopia using primary chicken embryo fibroblast (CEF) cell; which is time-consuming, laborious, and uneconomical. The present study was carried out to develop cell-based IBDV LC-75 vaccine using Vero cells, and to evaluate the immunogenicity and protection level.Results: Identity of the vaccine seed was confirmed using gene-specific primers using reverse transcription polymerase chain reaction. Confluent monolayer of Vero cells was infected with vaccine virus and serial passage continued till passage ten. Characteristic virus induced cytopathic effect was observed starting from passage 2 on the third day post-infection. The infectious titer of adapted virus showed a linear increment along the passage level. Virus induced specific antibody was determined using indirect ELISA after vaccination of 14 days old chicks through ocular route. Accordingly, the antibody titer measured from Vero cells vaccinated chicks revealed similar level with the currently available CEF cell-based vaccine. Chicks vaccinated with Vero cell adapted virus showed complete protection against very virulent IBDV, while unvaccinated group had 60% morbidity and 25% mortality.Conclusions: The IBDV vaccine strain well adapted on Vero cells and found to be immunogenic induces antibodies development and successfully protects chicks against challenging with the circulating field IBDV isolate. Hence, it is recommended to produce IBD vaccine using Vero cell culture with enough quantity to conquer the limitations using CEF cells and thus to vaccinate chicks to protect against IBDV infection.

scholarly journals Complete Genome Sequence of a Chicken Embryo Fibroblast-Adapted Attenuated Infectious Bursal Disease Virus Isolate from India

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
T. M. A. Senthilkumar ◽  
C. V. Priyadharsini ◽  
P. Raja ◽  
K. Kumanan
Keyword(s):  
Disease Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Infectious Bursal Disease ◽  
Avian Pathogen ◽  
Bursal Disease ◽  
Commercial Broiler

Infectious bursal disease virus is an avian pathogen that causes huge morbidity and mortality in the poultry sector all over the world. Here, we report the full-length genome sequence of an Indian strain, MB11/ABT/MVC/2016, isolated from a commercial broiler flock. This is a first report of a complete genome sequence of infectious bursal disease virus from India.

scholarly journals Replication of classical infectious bursal disease virus in the chicken embryo related cell line

2000 ◽  
Vol 29 (3) ◽  
pp. 213-217 ◽  
Author(s):  
T.C. Cardoso ◽  
P. Rahal ◽  
D. Pilz ◽  
M. C. B. Teixeira ◽  
C. W. Arns
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Related Cell ◽  
Bursal Disease

scholarly journals Growth and Replication of Infectious Bursal Disease Virus in the DF-1 Cell Line and Chicken Embryo Fibroblasts

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Kaliyaperumal Rekha ◽  
Chandran Sivasubramanian ◽  
Ill-Min Chung ◽  
Muthu Thiruvengadam
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Population Doubling ◽  
Cytopathic Effects ◽  
Bursal Disease ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

Infectious bursal disease virus (IBDV) causes a highly contagious disease in young chicks and leads to significant economic losses in the poultry industry. To determine a suitable cell line for IBDV infection, replication, and growth kinetics of the virus, DF-1 cells and chicken embryo fibroblasts (CEF) were used. The population doubling per day (Pd/D) was found to be higher in DF-1 as compared to CEF cells. A suitable time of infection (TOI) was established for increased production of virus and greater infectivity titers. The DF-1 and CEF cells were found to be susceptible to infection by producing marked cytopathic effects (CPEs), and the growth curves of IBDV in DF-1 and CEF cells were evaluated by infectivity assay using tissue culture infectious dose (TCID50). The cytopathic effects of the virus in DF-1 and CEF cells were found to be similar, but higher viral titers were detected in the DF-1 cells as compared to CEF. Thus the DF-1 cell line had a higher growth potential and infectivity, which will be of advantage in vaccine production.

Sign in / Sign up

Export Citation Format

Share Document