Effect of Infectious Bursal Disease Virus on in vitro Propagation of Chicken Embryo Fibroblast Cells

2005 ◽  
Vol 1 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Sarder Nasir Uddin ◽  
Sk. A. Hossain .
Keyword(s):  
Disease Virus ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
In Vitro Propagation ◽  
Chicken Embryo Fibroblast ◽  
Infectious Bursal Disease ◽  
Fibroblast Cells ◽  
Bursal Disease

Propagation of Infectious Bursal Disease Virus (IBDV) in Chicken Embryo Fibroblast Cells

2005 ◽  
Vol 6 (1) ◽  
pp. 146-149 ◽  
Author(s):  
SK. Amir Hossain . ◽  
Sarder Nasir Uddin . ◽  
Muhammad Sadiqur Rah . ◽  
Abdul Wadud . ◽  
Muhammad Harunuzzama .
Keyword(s):  
Disease Virus ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Infectious Bursal Disease ◽  
Fibroblast Cells ◽  
Bursal Disease

scholarly journals Effect of Force 6® Poultry on Infectious Bursal disease Virus in Vitro

2015 ◽  
Vol 39 (2) ◽  
pp. 87-90
Author(s):  
Amjed H. . Ulaiwi
Keyword(s):  
Tissue Culture ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Virus Titer ◽  
Infectious Bursal Disease ◽  
Virus Growth ◽  
Bursal Disease ◽  
And Control

     The aim of this study was to investigate the effect of force 6 poultry (conc.) in Log. of infectious bursal disease Virus on tissue culture and Virus, on Virus alone  and   on tissue culture alone. Different concentrations (0.5, 12.5, 25 and 50 µg/ml) were used to consider the anti-viral activity. The result showed effect of force 6® poultry (conc.) in Log. of Infectious Bursal Disease Virus. On tissue culture and Virus, the results revealed higher value with more significant (P˂0.05) differences at concentration (0.5 µg/ml) than other concentrations and control; On Virus alone, it showed more significant (P˂0.05) differences at concentration (0.5 µg/ml) than concentration (50 µg/ml) which showed less value of Virus growth. And on tissue culture alone (chicken embryo fibroblast) it showed lesser value and significant (P˂0.05) differences than other concentrations. In conclusion, the main changes in tissue culture explained at concentration (0.5, 25 and 50 µg/ml) but not (12.5 µg/ml and control). This group also were more affected on Virus titer when compared with other than two groups (tissue culture and Virus and Virus alone).

scholarly journals Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain in Ethiopia 

2021 ◽  
Author(s):  
Wakjira Kebebe ◽  
Molalegne Bitew ◽  
Fufa Dawo ◽  
Bedaso Mammo ◽  
Hawa Mohammed ◽  
...  
Keyword(s):  
Vero Cell ◽  
Disease Virus ◽  
Vaccine Strain ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Vero Cells ◽  
Infectious Bursal Disease ◽  
Efficacy Evaluation ◽  
Viral Pathogen ◽  
Bursal Disease

Abstract Background: Infectious bursal disease virus (IBDV) is an avian viral pathogen that causes infectious bursal disease (IBD) of chickens. The disease is endemic in Ethiopia since 2002 and vaccination is the major means of disease prevention and control. IBD vaccine is produced in Ethiopia using primary chicken embryo fibroblast (CEF) cell; which is time-consuming, laborious, and uneconomical. The present study was carried out to develop cell-based IBDV LC-75 vaccine using Vero cells, and to evaluate the immunogenicity and protection level.Results: Identity of the vaccine seed was confirmed using gene-specific primers using reverse transcription polymerase chain reaction. Confluent monolayer of Vero cells was infected with vaccine virus and serial passage continued till passage ten. Characteristic virus induced cytopathic effect was observed starting from passage 2 on the third day post-infection. The infectious titer of adapted virus showed a linear increment along the passage level. Virus induced specific antibody was determined using indirect ELISA after vaccination of 14 days old chicks through ocular route. Accordingly, the antibody titer measured from Vero cells vaccinated chicks revealed similar level with the currently available CEF cell-based vaccine. Chicks vaccinated with Vero cell adapted virus showed complete protection against very virulent IBDV, while unvaccinated group had 60% morbidity and 25% mortality.Conclusions: The IBDV vaccine strain well adapted on Vero cells and found to be immunogenic induces antibodies development and successfully protects chicks against challenging with the circulating field IBDV isolate. Hence, it is recommended to produce IBD vaccine using Vero cell culture with enough quantity to conquer the limitations using CEF cells and thus to vaccinate chicks to protect against IBDV infection.

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