Tissue Distribution and Relative Quantitation of Experimental Infection with Peste Des Petits Ruminant Virus in Goats Using Real-Time PCR (TaqMan®)

2012 ◽  
Vol 11 (16) ◽  
pp. 3011-3018 ◽  
Author(s):  
Xuepeng Cai ◽  
Xuelian Meng ◽  
Yongxi Dou ◽  
Junjun Zhai ◽  
Xiaoni Shi ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Real Time ◽  
Experimental Infection ◽  
Real Time Pcr ◽  
Relative Quantitation

Detection and relative quantitation of Soil-borne cereal mosaic virus (SBCMV) and Polymyxa graminis in winter wheat using real-time PCR (TaqMan®)

2004 ◽  
Vol 122 (1) ◽  
pp. 95-103 ◽  
Author(s):  
Claudio Ratti ◽  
Giles Budge ◽  
Lisa Ward ◽  
Gerard Clover ◽  
Concepcion Rubies-Autonell ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Mosaic Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Polymyxa Graminis ◽  
Relative Quantitation

Utilization of real-time pcr for the relative quantitation of MHC source-protein transcripts

2002 ◽  
Vol 63 (10) ◽  
pp. S6
Author(s):  
Mary E Ellexson-Turner ◽  
Heather D Hickman ◽  
Angela D Luis ◽  
Wilfried Bardet ◽  
William H Hildebrand
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Relative Quantitation

Development of a real-time PCR approach for the relative quantitation of horse DNA

2015 ◽  
Vol 7 (20) ◽  
pp. 8590-8596 ◽  
Author(s):  
Gavin J. Nixon ◽  
Timothy M. Wilkes ◽  
Malcolm J. Burns
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Horse Meat ◽  
Relative Quantitation ◽  
Processing Steps

Figure illustrating the basic processing steps required to identify and quantify potential horse meat adulteration.

scholarly journals Development of In Vitro resistance to fluoroquinolones in Pseudomonas aeruginosa

2020 ◽  
Author(s):  
Lei Zhao ◽  
Shiqi Wang ◽  
Xiaobing Li ◽  
Xiaojing He ◽  
Lingyan Jian
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Real Time ◽  
Real Time Pcr ◽  
Sanger Sequencing ◽  
Target Genes ◽  
Efflux Pump ◽  
Relative Quantitation ◽  
Evolutionary Trajectories ◽  
Quantitation Method

Abstract Background: The objectives of this study were to investigate the dynamics of different resistant mechanisms in P.aeruginosa populations that have evolved under fluoroquinolone pressure, and any interactions between these mechanisms in the evolutionary trajectories. Methods: In this study, bacteria of the strain ATCC27853 were selected under different concentrations of levofloxacin and ciprofloxacin for six parallel lineages. The four target genes in the quinolone-resistance determining region were amplified and then Sanger sequencing was used to find the mutations. The expression of four efflux pump proteins were evaluated by real-time PCR, using the relative quantitation method, and the ATCC27853 was selected as a control. Results: we found that the P.aeruginosa was killed by ciprofloxacin earlier than levofloxacin. We found five different mutations in three subunits of QRDRs in our study; gyrA was the main mutated gene for conferring resistance to fluoroquinolone. A greater number of mutations appeared at 4mg/L for levofloxacin and at 2mg/L for ciprofloxacin. The main efflux pump that was expressed was MexCD-OprJ, and the first over expressed was evident at 0.5mg/L for levofloxacin and 0.25mg/L for ciprofloxacin. Conclusions: The mutation of gyrA83 and overexpression of MexCD-OprJ were the main mechanisms that conferred resistance of P.aeruginosa to levofloxacin and ciprofloxacin. Ciprofloxacin had a stronger ability to kill the bacteria, while may render bacteria more susceptible to resistance.

scholarly journals Brazilian avian metapneumovirus subtypes A and B: experimental infection of broilers and evaluation of vaccine efficacy

2012 ◽  
Vol 32 (12) ◽  
pp. 1257-1262
Author(s):  
Márcia B. dos Santos ◽  
Matheus C. Martini ◽  
Helena L. Ferreira ◽  
Luciana H.A. da Silva ◽  
Paulo A. Fellipe ◽  
...  
Keyword(s):  
Real Time ◽  
Experimental Infection ◽  
Real Time Pcr ◽  
Vaccine Efficacy ◽  
Virus Isolation ◽  
Respiratory Pathogen ◽  
Avian Metapneumovirus ◽  
Subtype B ◽  
Challenge Experiment ◽  
Escape Mutants

Avian metapneumovirus (aMPV) is a respiratory pathogen associated with the swollen head syndrome (SHS) in chickens. In Brazil, live aMPV vaccines are currently used, but subtypes A and, mainly subtype B (aMPV/A and aMPV/B) are still circulating. This study was conducted to characterize two Brazilian aMPV isolates (A and B subtypes) of chicken origin. A challenge trial to explore the replication ability of the Brazilian subtypes A and B in chickens was performed. Subsequently, virological protection provided from an aMPV/B vaccine against the same isolates was analyzed. Upon challenge experiment, it was shown by virus isolation and real time PCR that aMPV/B could be detected longer and in higher amounts than aMPV/A. For the protection study, 18 one-day-old chicks were vaccinated and challenged at 21 days of age. Using virus isolation and real time PCR, no aMPV/A was detected in the vaccinated chickens, whereas one vaccinated chicken challenged with the aMPV/B isolate was positive. The results showed that aMPV/B vaccine provided a complete heterologous virological protection, although homologous protection was not complete in one chicken. Although only one aMPV/B positive chicken was detected after homologous vaccination, replication in vaccinated animals might allow the emergence of escape mutants.

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