scholarly journals Cloning and Characterization of Oviduct Tubular Gland Cell Tissue-Specific Promoters of White Leghorn Chicken

2012 ◽  
Vol 11 (10) ◽  
pp. 1656-1665
Author(s):  
Jenn-Fa Liou ◽  
Chang-Hsin Chan ◽  
Jen-Wen Shiau ◽  
Jui-Jane Tailiu ◽  
Ming-Cheng Chang ◽  
...  
Keyword(s):  
Gland Cell ◽  
White Leghorn ◽  
Tissue Specific ◽  
White Leghorn Chicken ◽  
Cell Tissue ◽  
Tubular Gland

Designing, Construction and Functional Characterization of Tissue-specific Synthetic Promoter in Rice

2017 ◽  
Vol 43 (6) ◽  
pp. 789
Author(s):  
Rui WANG ◽  
Meng-Lin ZHU ◽  
Fang-Yuan GAO ◽  
Juan-Sheng REN ◽  
Xian-Jun LU ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Synthetic Promoter ◽  
Tissue Specific

scholarly journals Syntaxin 1A Gene Is Negatively Regulated in a Cell/Tissue Specific Manner by YY1 Transcription Factor, Which Binds to the −183 to −137 Promoter Region Together with Gene Silencing Factors Including Histone Deacetylase

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 146
Author(s):  
Takahiro Nakayama ◽  
Toshiyuki Fukutomi ◽  
Yasuo Terao ◽  
Kimio Akagawa
Keyword(s):  
Transcription Factor ◽  
Gene Silencing ◽  
Protein Complex ◽  
Promoter Region ◽  
Neuronal Cell ◽  
Syntaxin 1A ◽  
Tissue Specific ◽  
Cell Tissue ◽  
Specific Manner ◽  
A Cell

The HPC-1/syntaxin 1A (Stx1a) gene, which is involved in synaptic transmission and neurodevelopmental disorders, is a TATA-less gene with several transcription start sites. It is activated by the binding of Sp1 and acetylated histone H3 to the −204 to +2 core promoter region (CPR) in neuronal cell/tissue. Furthermore, it is depressed by the association of class 1 histone deacetylases (HDACs) to Stx1a–CPR in non-neuronal cell/tissue. To further clarify the factors characterizing Stx1a gene silencing in non-neuronal cell/tissue not expressing Stx1a, we attempted to identify the promoter region forming DNA–protein complex only in non-neuronal cells. Electrophoresis mobility shift assays (EMSA) demonstrated that the −183 to −137 OL2 promoter region forms DNA–protein complex only in non-neuronal fetal rat skin keratinocyte (FRSK) cells which do not express Stx1a. Furthermore, the Yin-Yang 1 (YY1) transcription factor binds to the −183 to −137 promoter region of Stx1a in FRSK cells, as shown by competitive EMSA and supershift assay. Chromatin immunoprecipitation assay revealed that YY1 in vivo associates to Stx1a–CPR in cell/tissue not expressing Stx1a and that trichostatin A treatment in FRSK cells decreases the high-level association of YY1 to Stx1a-CPR in default. Reporter assay indicated that YY1 negatively regulates Stx1a transcription. Finally, mass spectrometry analysis showed that gene silencing factors, including HDAC1, associate onto the −183 to −137 promoter region together with YY1. The current study is the first to report that Stx1a transcription is negatively regulated in a cell/tissue-specific manner by YY1 transcription factor, which binds to the −183 to −137 promoter region together with gene silencing factors, including HDAC.

Characterization of microRNAs from neem (Azadirachta indica) and their tissue-specific expression study in leaves and stem

3 Biotech ◽  
2021 ◽  
Vol 11 (6) ◽  
Author(s):  
Sujay Paul ◽  
Paula Reyes-Pérez ◽  
Paola Isabel Angulo-Bejarano ◽  
Aashish Srivastava ◽  
Sathishkumar Ramalingam ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Expression Study ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Quantitative and Qualitative Protein Changes in the Developing Single Comb White Leghorn Chicken Embryo

1972 ◽  
Vol 51 (1) ◽  
pp. 313-320 ◽  
Author(s):  
J.A. Asmar ◽  
P.L. Pellett ◽  
Nur Hariri ◽  
M.D. Hariri
Keyword(s):  
Chicken Embryo ◽  
White Leghorn ◽  
White Leghorn Chicken ◽  
Single Comb

Characterization of G3BPs: Tissue specific expression, chromosomal localisation andrasGAP120 binding studies

2001 ◽  
Vol 84 (1) ◽  
pp. 173-187 ◽  
Author(s):  
Derek Kennedy ◽  
Juliet French ◽  
Estelle Guitard ◽  
Kelin Ru ◽  
Bruno Tocque ◽  
...  
Keyword(s):  
Binding Studies ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Chromosomal Localisation

Development and characterization of a chimaeric tissue-specific promoter in wheat and rice endosperm

2007 ◽  
Vol 44 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Maria Oszvald ◽  
Mark Gardonyi ◽  
Cecília Tamas ◽  
Imre Takacs ◽  
Barnabas Jenes ◽  
...  
Keyword(s):  
Tissue Specific ◽  
Rice Endosperm ◽  
Tissue Specific Promoter
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