scholarly journals Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein

2014 ◽  
Vol 10 (3) ◽  
pp. 243-252
Author(s):  
Ales Eichmeier
Keyword(s):  
Mosaic Virus ◽  
Movement Protein ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Virus Isolates

scholarly journals Effect of fungicide farmayod on agrotechnical and technological indicators of grapevine, on viral diseases and oidium

2021 ◽  
Vol 273 ◽  
pp. 01020
Author(s):  
Nadezda Sirotkina
Keyword(s):  
Mosaic Virus ◽  
Drug Concentration ◽  
Viral Diseases ◽  
Cabernet Sauvignon ◽  
Virus Concentration ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Drug Concentrations ◽  
Leaf Virus ◽  
Oidium Tuckeri

The paper presents the study on the effect of Farmayod’s GR (100 g/l of iodine) spraying on vineyards of Cabernet Sauvignon and Baklanovsky varieties on the degree of viral and oidium prevalence as well as on agrobiological and technological indicators. According to the aggregate agrobiological and technological indicators, the best results on Cabernet Sauvignon variety were obtained when the drug was used at a concentration of 0.06 %. On the Baklanovsky variety the best indicators were obtained at a drug concentration of 0.04%. Testing of plant samples for the presence of Grapevine fan leaf virus, Arabis mosaic virus and Oidium tuckeri showed that after two years of applying the drug, the prevalence of infected plants (P, %) with Grapevine fanleaf virus on the Cabernet Sauvignon cultivar varied from 0% (fungicide concentration 0.04 and 0.05 %) to 0.8 % (0.06 %) and 2.65 % (control). For Baklanovsky variety: Grapevine fanleaf virus - concentration 0.04 % - 1.8; 0.05 % - 0.4; 0.06 % - 2.0; control - 2.65 %. Arabis mosaic virus – 0; 0; 3.0; 12.1 %, respectively. Oidium tuckeri was 0 % in all variants with any drug concentrations. Control variant and later 80 % for 29.09.

scholarly journals Mapping of the occurrence of grapevine viruses: GFLV and ArMV in vineyards of the grapevine breeding station Polešovice

2007 ◽  
Vol 55 (5) ◽  
pp. 117-122
Author(s):  
Pavel Pavloušek
Keyword(s):  
Czech Republic ◽  
Mosaic Virus ◽  
Elisa Test ◽  
Grapevine Fanleaf Virus ◽  
Virus Diseases ◽  
Arabis Mosaic Virus ◽  
Good Basis ◽  
Worldwide Distribution ◽  
Breeding Station ◽  
Positive Results

Due to their worldwide distribution and also detrimental potential virus diseases of grapevine are economically very important. In grapevine plants Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) is one of the most harmful virus pathogens. The aim of this study was evaluate the occurrence of GFLV and ArMV in vineyards of the Grapevine Breeding Station Polešovice (Czech Republic) using the ELISA test and visual symptoms. In every year from 2001–2004 altogether 225 grapevine plants were tested for the occurrence of GFLV and ArMV. In case of GFLV positive results were obtained in 43 plants, i. e. in 19.11% of all samples. In case of ArMV there were 51 positive tests, i.e. 22.66%. Our results demonstrated a occurrence of GFLV and ArMV in propagation vineyards, which was comparable with results obtained abroad. It was also demonstrated that a professional visual selection could provide a good basis for the identification of virus diseases and their subsequent tests.

scholarly journals Identification and characterization of a new potyvirus infecting Muscari in Hungary

2020 ◽  
Author(s):  
János Ágoston ◽  
Asztéria Almási ◽  
Katalin Salánki ◽  
László Palkovics
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Tobacco Rattle Virus ◽  
Coding Region ◽  
Arabis Mosaic Virus ◽  
Potyvirus Species ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Grape Hyacinth ◽  
Virus Isolates

AbstractGrape hyacinths (Muscari spp.) are popular spring flowering bulbs in Europe and also in Hungary. In the spring of 2017, we came across grape hyacinth plants showing mosaic symptoms, which indicated viral infection. Currently Hyacinth mosaic virus (genus Potyvirus), a proposed member of the genus named Muscari mosaic virus, Arabis mosaic virus (genus Nepovirus), Cucumber mosaic virus (genus Cucumovirus) and Tobacco rattle virus (genus Tobravirus) are known to infect grape hyacinth. Leaf samples of symptomatic grape hyacinths were observed, collected and the presence of potyviruses was proved with potyvirus specific monoclonal antibody by ELISA and by potyvirus specific RT-PCR. Laboratory host plants and seed grown Muscari plants were inoculated with leaf sap and symptoms were recorded. Nucleotide sequences of the cloned fragments were compared to GenBank data. In the case of Muscari ‘Helena’ the highest nucleotide identity in the coat protein coding region was found with two Muscari mosaic virus isolates (95.51% and 95.79%). In the case of the clones derived from Muscari ‘Pink Sunrise’ plant, the highest identity was recorded with Muscari mosaic virus isolates (57.65% and 57.80%) and with a Tulip breaking virus strain (55.13%) indicating the existence of a novel potyvirus species, tentatively named Muscari chlorotic mottle virus. The coat protein sequences were aligned and Maximum Likelihood trees were built to analyze phylogenetic relationships.

scholarly journals Diversity of Apple mosaic virus Isolates in India Based on Coat Protein and Movement Protein Genes

2011 ◽  
Vol 22 (1) ◽  
pp. 44-49 ◽  
Author(s):  
Vijay Lakshmi ◽  
Vipin Hallan ◽  
Raja Ram ◽  
Nazeer Ahmed ◽  
A. A. Zaidi ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Virus Isolates

scholarly journals Sanitary status of slovenian indigenous grapevine cultivar Refosk

OENO One ◽  
2005 ◽  
Vol 39 (1) ◽  
pp. 19 ◽  
Author(s):  
Irma Tomazic ◽  
Zora Korosec-Koruza ◽  
Natasa Petrovic
Keyword(s):  
Vitis Vinifera ◽  
Mosaic Virus ◽  
Vitis Vinifera L ◽  
Grapevine Virus ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Disease Symptoms ◽  
Grapevine Cultivar ◽  
Grapevine Virus A ◽  
Grapevine Virus B

<p style="text-align: justify;">Sanitary status of visually selected and nonselected indigenous grapevine cultivar 'Refosk' (Vitis vinifera L. cv. 'Refosk') was evaluated. Nine viruses were tested by ELISA: Nepoviruses Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV), Closteroviruses Grapevine leafroll associated viruses 1 (GLRaV-1), 2 (GLRaV-2), 3 (GLRaV-3), and 6 (GLRaV-6), Grapevine fleck virus (GFkV) and Vitiviruses Grapevine virus A (GVA) and Grapevine virus B (GVB). All viruses except ArMV and GVB were detected. The vines of cv. ‘Refosk’ passing the visual selection were 50 % free of tested viruses in comparison to only 24 % virus-free vines of non-visually selected material. Visually selected plants revealed the highest incidence of virus GLRaV- 1 (37 %) and in addition to that a 15 % incidence of rugose wood (RW) disease symptoms.</p>

scholarly journals Detecção e identificação molecular de vírus associados a videiras sintomáticas e assintomáticas

2010 ◽  
Vol 40 (11) ◽  
pp. 2249-2255 ◽  
Author(s):  
Marcos Fernando Basso ◽  
Thor Vinícius Martins Fajardo ◽  
Marcelo Eiras ◽  
Ricardo Antônio Ayub ◽  
Osmar Nickel
Keyword(s):  
Mosaic Virus ◽  
Grapevine Virus ◽  
Rt Pcr ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Para Determinar ◽  
Rupestris Stem Pitting ◽  
Stem Pitting

A propagação vegetativa da videira favorece infecções virais múltiplas, com expressão diferencial de sintomas em função da combinação da cultivar ou espécie da hospedeira com a espécie viral. Os objetivos deste trabalho foram detectar e identificar as espécies virais presentes em duas espécies/cultivares de videira: uma sintomática e outra assintomática. DsRNA de ambas as amostras foi submetido à RT-PCR com 17 pares de oligonucleotídeos específicos para a detecção de Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine fleck virus (GFkV), Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Grapevine chrome mosaic virus (GCMV), Rupestris stem pitting-associated virus (RSPaV), Grapevine leafroll-associated virus 1 a 4 (GLRaV-1 a -4), além de três pares de oligonucleotídeos degenerados. Pelo menos um fragmento amplificado, por par de oligonucleotídeos, foi clonado e sequenciado. Plantas sintomáticas e assintomáticas mostraram infecções múltiplas por RSPaV, GLRaV-2 e/ou GLRaV-3. As sequências de nucleotídeos obtidas para sete isolados de RSPaV, três de GLRaV-2 e dois de GLRaV-3 apresentaram identidades superiores a 90% com espécies homólogas e permitiram a definição das possíveis estirpes presentes nas amostras infectadas. Esses resultados evidenciam a necessidade da diagnose viral baseada em testes específicos para determinar a condição sanitária da videira.

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