scholarly journals Isolation of Fluorescent Pseudomonas spp. from Diverse Agro-Ecosystems of India and Characterization of their PGPR Traits

2014 ◽  
Vol 5 (1) ◽  
pp. 13-24 ◽  
Author(s):  
G. Praveen Kumar ◽  
Suseelendra Desai ◽  
E. Leo Daniel Amalraj ◽  
Gopal Reddy
Keyword(s):  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Pgpr Traits

Evaluation of fluorescent Pseudomonas spp. with single and multiple PGPR traits for plant growth promotion of sorghum in combination with AM fungi

2012 ◽  
Vol 67 (2) ◽  
pp. 133-140 ◽  
Author(s):  
G. Praveen Kumar ◽  
N. Kishore ◽  
E. Leo Daniel Amalraj ◽  
S. K. Mir Hassan Ahmed ◽  
Abdul Rasul ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Am Fungi ◽  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Pgpr Traits

Physiological and genetic characterization of fluorescentPseudomonasassociated withCantharellus cibarius

2002 ◽  
Vol 48 (8) ◽  
pp. 739-748 ◽  
Author(s):  
J Ignacio Rangel-Castro ◽  
Jolanta J Levenfors ◽  
Eric Danell
Keyword(s):  
16S Rdna ◽  
Fungal Mycelium ◽  
Sequencing Analysis ◽  
Carbon Utilization ◽  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Rdna Sequencing ◽  
Pcr Rflp ◽  
Polymerase Chain

Fluorescent Pseudomonas spp. isolated from fruiting bodies (FB) of Cantharellus cibarius were characterized physiologically and genetically and were compared with fluorescent Pseudomonas from forest soil and with sequences from the GenBank database. Pseudomonas spp. from FB differed physiologically from isolates from soil lacking FB and had some similarities with the strains obtained from soil underneath the FB. Analyses of the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) patterns and partial sequencing analysis of the 16S-rDNA region indicated that the bacteria isolated from these environments were different. However, there was no specific Pseudomonas genotype restricted to the FB environment. Utilization of the reported fungal exudates trehalose and mannitol may explain how millions of bacteria survive in the C. cibarius FB without deteriorating the fungal mycelium. The importance of the metabolic characterization of bacteria and the possible mechanisms involved in the association with C. cibarius are discussed. Our study showed that standard processes for bacterial identification, e.g., Biolog®and 16S-rDNA are insufficient until databases for different ecosystems are created.Key words: Cantharellus cibarius, fluorescent Pseudomonas, carbon utilization, PCR–RFLP, 16S-rDNA sequencing.

scholarly journals Characterization of fluorescent Pseudomonas spp. associated with roots and soil of two sorghum genotypes

2013 ◽  
Vol 136 (3) ◽  
pp. 469-481 ◽  
Author(s):  
Deanna L. Funnell-Harris ◽  
Scott E. Sattler ◽  
Jeffrey F. Pedersen
Keyword(s):  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Sorghum Genotypes

scholarly journals Characterization of Pseudomonas spp. and Associated Proteolytic Properties in Raw Milk Stored at Low Temperatures

2017 ◽  
Vol 8 ◽  
Author(s):  
Lu Meng ◽  
Yangdong Zhang ◽  
Huimin Liu ◽  
Shengguo Zhao ◽  
Jiaqi Wang ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Raw Milk ◽  
Pseudomonas Spp

scholarly journals Phosphate Solubilization Potential of Fluorescent Pseudomonas spp. Isolated from Diverse Agro-Ecosystems of India

2014 ◽  
Vol 9 (3) ◽  
pp. 101-110 ◽  
Author(s):  
Praveen Kumar Goteti ◽  
Suseelendra Desai ◽  
Leo Daniel Amalraj Em ◽  
Minakshi Taduri ◽  
Uzma Sultana
Keyword(s):  
Phosphate Solubilization ◽  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas

scholarly journals Effect of Population Density of Pseudomonas fluorescens on Production of 2,4-Diacetylphloroglucinol in the Rhizosphere of Wheat

1999 ◽  
Vol 89 (6) ◽  
pp. 470-475 ◽  
Author(s):  
Jos M. Raaijmakers ◽  
Robert F. Bonsall ◽  
David M. Weller
Keyword(s):  
Biological Control ◽  
Population Density ◽  
Supporting Evidence ◽  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Naturally Occurring ◽  
Rhizosphere Population ◽  
First Time ◽  
Take All

The role of antibiotics in biological control of soilborne pathogens, and more generally in microbial antagonism in natural disease-suppressive soils, often has been questioned because of the indirect nature of the supporting evidence. In this study, a protocol for high pressure liquid chromatography/mass spectrometry is described that allowed specific identification and quantitation of the antibiotic 2,4-diacetylphloroglucinol (Phl) produced by naturally occurring fluorescent Pseudomonas spp. on roots of wheat grown in a soil suppressive to take-all of wheat. These results provide, for the first time, biochemical support for the conclusion of previous work that Phl-producing fluorescent Pseudomonas spp. are key components of the natural biological control that operates in take-all—suppressive soils in Washington State. This study also demonstrates that the total amount of Phl produced on roots of wheat by P. fluorescens strain Q2-87, at densities ranging from approximately 105 to 107 CFU/g of root, is proportional to its rhizosphere population density and that Phl production per population unit is a constant (0.62 ng/105 CFU). Thus, Phl production in the rhizosphere of wheat is strongly related to the ability of the introduced strain to colonize the roots.

scholarly journals Biological Control of Take-All by Fluorescent Pseudomonas spp. from Chinese Wheat Fields

2011 ◽  
Vol 101 (12) ◽  
pp. 1481-1491 ◽  
Author(s):  
Ming-Ming Yang ◽  
Dmitri V. Mavrodi ◽  
Olga V. Mavrodi ◽  
Robert F. Bonsall ◽  
James A. Parejko ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Restriction Analysis ◽  
Gaeumannomyces Graminis ◽  
Mass Spectrometry Analysis ◽  
In Vitro Tests ◽  
Spectrometry Analysis ◽  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Take All

Take-all disease of wheat caused by the soilborne fungus Gaeumannomyces graminis var. tritici is one of the most important root diseases of wheat worldwide. Bacteria were isolated from winter wheat from irrigated and rainfed fields in Hebei and Jiangsu provinces in China, respectively. Samples from rhizosphere soil, roots, stems, and leaves were plated onto King's medium B agar and 553 isolates were selected. On the basis of in vitro tests, 105 isolates (19% of the total) inhibited G. graminis var. tritici and all were identified as Pseudomonas spp. by amplified ribosomal DNA restriction analysis. Based on biocontrol assays, 13 strains were selected for further analysis. All of them aggressively colonized the rhizosphere of wheat and suppressed take-all. Of the 13 strains, 3 (HC9-07, HC13-07, and JC14-07, all stem endophytes) had genes for the biosynthesis of phenazine-1-carboxylic acid (PCA) but none had genes for the production of 2,4-diacetylphloroglucinol, pyoluteorin, or pyrrolnitrin. High-pressure liquid chromatography (HPLC) analysis of 2-day-old cultures confirmed that HC9-07, HC13-07, and JC14-07 produced PCA but no other phenazines were detected. HPLC quantitative time-of-flight 2 mass-spectrometry analysis of extracts from roots of spring wheat colonized by HC9-07, HC13-07, or Pseudomonas fluorescens 2-79 demonstrated that all three strains produced PCA in the rhizosphere. Loss of PCA production by strain HC9-07 resulted in a loss of biocontrol activity. Analysis of DNA sequences within the key phenazine biosynthesis gene phzF and of 16S rDNA indicated that strains HC9-07, HC13-07, and JC14-07 were similar to the well-described PCA producer P. fluorescens 2-79. This is the first report of 2-79-like bacteria being isolated from Asia.

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