scholarly journals Characterization of Pseudomonas spp. and Associated Proteolytic Properties in Raw Milk Stored at Low Temperatures

2017 ◽  
Vol 8 ◽  
Author(s):  
Lu Meng ◽  
Yangdong Zhang ◽  
Huimin Liu ◽  
Shengguo Zhao ◽  
Jiaqi Wang ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Raw Milk ◽  
Pseudomonas Spp

Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Fatima N. Aziz ◽  
Laith Abdul Hassan Mohammed-Jawad
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Raw Milk ◽  
Staphylococcal Enterotoxin B ◽  
Human Pathogen ◽  
Conventional Pcr ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

Characterization of a novel theta-type plasmid pSM409 of Enterococcus faecium RME isolated from raw milk

Gene ◽  
2021 ◽  
Vol 777 ◽  
pp. 145459
Author(s):  
Tawsif Ahmed Kazi ◽  
Suranjita Mitra ◽  
Bidhan Chandra Mukhopadhyay ◽  
Sukhendu Mandal ◽  
Swadesh Ranjan Biswas
Keyword(s):  
Enterococcus Faecium ◽  
Raw Milk

Characterization of a thermoelectric generator at low temperatures

2012 ◽  
Vol 62 ◽  
pp. 47-50 ◽  
Author(s):  
Sevan Karabetoglu ◽  
Altug Sisman ◽  
Z. Fatih Ozturk ◽  
Turker Sahin
Keyword(s):  
Low Temperatures ◽  
Thermoelectric Generator

scholarly journals Isolation and Characterization of Bacteriophage ZCSE6 against Salmonella spp.: Phage Application in Milk

Biologics ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 164-176
Author(s):  
Abdallah S. Abdelsattar ◽  
Anan Safwat ◽  
Rana Nofal ◽  
Amera Elsayed ◽  
Salsabil Makky ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Raw Milk ◽  
Salmonella Spp ◽  
Isolation And Characterization ◽  
Food Borne ◽  
Wide Range ◽  
Almost All ◽  
And Control ◽  
Milk And Dairy Products

Food safety is very important in the food industry as most pathogenic bacteria can cause food-borne diseases and negatively affect public health. In the milk industry, contamination with Salmonella has always been a challenge, but the risks have dramatically increased as almost all bacteria now show resistance to a wide range of commercial antibiotics. This study aimed to isolate a bacteriophage to be used as a bactericidal agent against Salmonella in milk and dairy products. Here, phage ZCSE6 has been isolated from raw milk sample sand molecularly and chemically characterized. At different multiplicities of infection (MOIs) of 0.1, 0.01, and 0.001, the phage–Salmonella interaction was studied for 6 h at 37 °C and 24 h at 8 °C. In addition, ZCSE6 was tested against Salmonella contamination in milk to examine its lytic activity for 3 h at 37 °C. The results showed that ZCSE6 has a small genome size (<48.5 kbp) and belongs to the Siphovirus family. Phage ZCSE6 revealed a high thermal and pH stability at various conditions that mimic milk manufacturing and supply chain conditions. It also demonstrated a significant reduction in Salmonella concentration in media at various MOIs, with higher bacterial eradication at higher MOI. Moreover, it significantly reduced Salmonella growth (MOI 1) in milk, manifesting a 1000-fold decrease in bacteria concentration following 3 h incubation at 37 °C. The results highlighted the strong ability of ZCSE6 to kill Salmonella and control its growth in milk. Thus, ZCSE6 is recommended as a biocontrol agent in milk to limit bacterial growth and increase the milk shelf-life.

Preparation and Characterization of Pt/Al-ZSM-5 Catalysts and their Reactivities for the Oxidation of CO with N2O at Low Temperatures

2005 ◽  
Vol 102 (3-4) ◽  
pp. 149-152 ◽  
Author(s):  
Masaya Matsuoka ◽  
Kiyoshi Iino ◽  
Haijun Chen ◽  
Yukiko Yamasaki ◽  
Gianmario Marta ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Oxidation Of Co

Einfluss von Silan-dotierten Umgebungsatmosphären auf tribologischen Eigenschaften von Titan

2021 ◽  
Author(s):  
Selina Raumel ◽  
Khemais Barienti ◽  
Folke Dencker ◽  
Florian Nürnberger ◽  
Marc C. Wurz
Keyword(s):  
Low Temperatures ◽  
Surface Oxidation ◽  
Wear Volume ◽  
Ambient Atmosphere ◽  
Free Atmosphere ◽  
Metalworking Industry ◽  
Tribological System ◽  
Identification And Characterization ◽  
Ball On Disc

The production processes of the metalworking industry are usually carried out in the presence of oxygen. Par ticularly due to surface oxidation on tools and parts during production under normal atmosphere, high wear is observed in tribological systems. The focus of these investigations is the question to what extent the ambient atmosphere has an influence on the tribological system. For this purpose, the identification and characterization of wear mechanisms in an oxygen-free atmosphere is of high relevance. To analyse the influence of the ambi ent atmosphere on the tribological properties of titanium, ball-on-disc investigations are carried out on a univer sal tribometer (UMT) in an air, argon and silane-anodized atmosphere. By suppressing the oxidation under ex clusion of oxygen, a reduction of the tribochemical wear could be shown, which is accompanied by a reduction of the wear volume by a Factor of 4.5. However, due to the adhesion of both friction partners, which already occurs at low temperatures, caused by the omission of the friction-reducing cover layers, the friction coefficient increased. In addition, novel alloy formations at the interfaces were detected and analysed.

scholarly journals MOLECULAR DETECTION AND CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 277-282 ◽  
Author(s):  
M. A. Islam ◽  
S. M. L. Kabir ◽  
M. T. Rahman
Keyword(s):  
Molecular Detection ◽  
Culture Media ◽  
Raw Milk ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Selective Media ◽  
Milk Samples ◽  
Antimicrobial Sensitivity ◽  
Antimicrobial Sensitivity Test

The study was intended for molecular detection of S. aureus isolated from raw cow’s milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found to be positive for S. aureus. S. aureus specific 16S rRNA gene was amplified from all isolates and identified as S. aureus. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the S. aureus isolates were resistant to amoxicillin (100%), erythromycin (73.33%) and tetracycline (73.33%) but sensitive to azithromycin (93.33%), ciprofloxacin (93.33%), gentamicin (100%), norfloxacin (86.67%) and streptomycin (86.67%).

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