Multi-mycotoxin profiling in maize reveals prevalence of Fusarium mycotoxins in South and West Ethiopia

2021 ◽  
pp. 1-12
Author(s):  
A. Mesfin ◽  
K. Tesfamariam ◽  
T. Belachew ◽  
S. De Saeger ◽  
C. Lachat ◽  
...  
Keyword(s):  
Animal Feed ◽  
Animal Health ◽  
Special Focus ◽  
Fusarium Mycotoxins ◽  
Ecological Zones ◽  
Mycotoxin Contamination ◽  
Protective Strategies ◽  
Contamination Levels ◽  
Aflatoxin B ◽  
Study Participants

Multi-mycotoxin exposure data are missing to guide risk assessment and legislation in Ethiopia. This study therefore aimed to determine mycotoxin contamination levels in maize samples from 176 randomly selected household storages in three agro ecological zones of South (Sidama zone) and West (Jimma zone) Ethiopia, and to examine the post-harvest practices and household processing. Liquid chromatography coupled to tandem mass spectrometry was used to quantify 23 mycotoxins. The majority of the households regularly consumed maize (more than once per day). More (77%) samples in Sidama were contaminated with Fusarium mycotoxins deoxynivalenol than in Jimma (29%) (P<0.001); Similar distributions of fumonisin B1 (19%), fumonisin B2 (19%) and fumonisin B3 (12%, Sidama vs 13%, Jimma) contamination were observed (P>0.05). In Sidama, only one sample was contaminated with the Aspergillus mycotoxin aflatoxin B2 and another sample with aflatoxin B1. From all samples, 40% were contaminated with 3-5 types of Fusarium mycotoxins and only 4% of the samples were contaminated with 6-8 types of mycotoxins. After the harvested maize was dried on the field, the majority of respondents in Jimma reported that they removed the maize within one day, which was less practiced in Sidama. The majority of households in Sidama, and some in Jimma, reported that they dried maize before storage, mainly using the sun. Close to two third of the study participants in the two zones reported that they applied the chemical dichloro-diphenyl-trichloroethane (DDT) during maize storage. All households in both zones reported that they sorted visible mouldy maize grains before preparation of maize flour while most of them kept the mouldy maize for animal feed. Protective strategies of Fusarium mycotoxin contamination, with special focus on deoxynivalenol and zearalenone, should be well promoted in the study areas as they are possible human and animal health threats.

The recent Fusarium mycotoxin situation in grain and feed in China

2015 ◽  
Vol 8 (5) ◽  
pp. 545-551 ◽  
Author(s):  
W.W. Zhang ◽  
X.D. Deng ◽  
X.P. Yu ◽  
X.F. Pei ◽  
G.M. Fu ◽  
...  
Keyword(s):  
North China ◽  
Animal Feed ◽  
Animal Health ◽  
Incidence Rates ◽  
Central China ◽  
Fusarium Mycotoxins ◽  
Fusarium Mycotoxin ◽  
Mycotoxin Contamination ◽  
Legal Limits ◽  
Feed Samples

Fusarium mycotoxins, such as trichothecenes, zearalenone (ZEA) and fumonisins, are widely distributed in grain and animal feed and cause hazards to human and animal health. China, one of the largest producers of agricultural products and animal feed, constantly faces challenges in preventing and controlling Fusarium mycotoxins. The recent status of Fusarium mycotoxins in grain and feed is of interest to many stakeholders; however, no comprehensive review of this has been published to date. The objective of this article is to review the recent situation in China, including the contamination situation, its (probable) main causes and the updated regulations. Every district of China has been affected by Fusarium mycotoxin contamination to varying degrees, with the most seriously affected districts being East China, Central China and North China. The incidence rates of deoxynivalenol and ZEA were higher than those of other Fusarium mycotoxins in both grain and feed samples. It has been suggested that deoxynivalenol-3-glucoside should undergo the risk assessment and the development of a related legal limit in China. Among the multiple causes of Fusarium mycotoxin occurrence in China, geography and climate and the variable characteristics of plants are probably the two important causes. The latest legal limits for Fusarium mycotoxins in food were promulgated in 2011, and the legal limits in feed are in the process of being revised. This article aims to provide information for promoting an understanding of the recent situation and the challenges for combating Fusarium mycotoxin contamination of grain and feed in China.

scholarly journals Milk Marketing and Dairy Value Chain Development in Nepal in Relation with Climate Resilience Effort in the Present Context

2017 ◽  
Vol 34 ◽  
pp. 144-151 ◽  
Author(s):  
B. Sharma
Keyword(s):  
Milk Production ◽  
Value Chain ◽  
Animal Feed ◽  
Health Hazard ◽  
Animal Health ◽  
Development Program ◽  
Seasonal Fluctuation ◽  
Product Diversification ◽  
Special Focus ◽  
Livestock Development

Nepal is agricultural country with a special focus on dairy sub-sector. Almost 80% of milk in market, which have been produced by small holder farmers with a well- developed dairy value chain. Ministry of Livestock Development (MoLD) has given due importance for milk production providing support on feed and forage programs to the farmers as well as introducing crossbreeding programs to uplift the genetics of local cattle and buffaloes. By 2018, the country shall be self-sufficient in milk production, where the requirement of 91 lit of milk per person per year is expected to be achieved. Foot and Mouth Disease (FMD) emerges as a major health hazard in dairy sector apart from Hemorrhagic Septicemia (HS) and black quarter (BQ). The incidence of clinical and subclinical mastitis is still high in dairy pocket areas. Moreover, the seasonal fluctuation of milk production is hampering the growth of dairy sector. The need of product diversification and powder milk production are of immense important in long run to sustain dairy sub-sector in Nepal. MOLD has given priority for four pillars for livestock development namely animal feed, animal breeds, animal health and livestock marketing. That sector on balanced development program will lead to increase on milk, meat, eggs, nutrition, and food security in the nation.

Occurrence of mycotoxins in aquaculture: preliminary overview of Asian and European plant ingredients and finished feeds

2017 ◽  
Vol 10 (2) ◽  
pp. 183-194 ◽  
Author(s):  
R.A. Gonçalves ◽  
D. Schatzmayr ◽  
U. Hofstetter ◽  
G.A. Santos
Keyword(s):  
Raw Materials ◽  
Canola Meal ◽  
Fusarium Mycotoxins ◽  
Plant Raw Materials ◽  
Plant Protein Sources ◽  
Corn Gluten ◽  
Contamination Levels ◽  
One Year ◽  
Aflatoxin B ◽  
Plant Sources

Concern about mycotoxins in aquaculture has been growing, partly due to the gradual replacement of animal-derived proteins, such as fish meal, by plant sources. Over a period of one year, 2,176 samples of different plant protein sources and 25 samples of finished aquaculture feeds were analysed. Samples were tested for aflatoxins (AF; sum of aflatoxin B1, B2, G1 and G2), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB; sum of fumonisin B1 and B2), T-2 toxin and ochratoxin A (full toxin screen). The following plant-based meals were analysed: soybean meal (SBM), wheat (WH), wheat bran (WB), maize (C), corn gluten meal (CGM), cottonseed meal (CSM), rapeseed/canola meal (R/CM) and rice bran (RB). The plant raw materials and aquaculture finished feeds were obtained from Asia and Europe. Finished feed samples from Asia were acquired from Vietnam, Indonesia and Myanmar, while from Europe they were sampled from Denmark, Austria, the Netherlands and Germany. Mycotoxins were found in most of the commodities and finished feeds analysed, showing that mycotoxins represent a risk for the development of the aquaculture sector. Generally, in Asian samples we observed that SBM, WH, WB, C, CGM, R/CM and RB were mostly contaminated with Fusarium mycotoxins (ZEA, DON and FB). CSM was contaminated primarily by AF and Fusarium toxins (ZEA and DON) at lower concentrations. European samples were contaminated mainly by Fusarium mycotoxins. The co-occurrence of mycotoxins in all commodities was high, raising the probability of co-occurrence in finished feeds. An accumulation of mycotoxins on the processed plant-based ingredients (e.g. CGM and WB) was observed when compared to the respective whole grains (C and WH, respectively). Compared to results obtained in 2014, finished feeds presented lower contamination levels, but the co-occurrence risk increased.

69 Occurrence of Mycotoxins in 2020 US Corn Grain and Corn By-product Feeds

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 36-37
Author(s):  
Paige N Gott ◽  
Erin Schwandt ◽  
Shelby M Ramirez ◽  
Ursula Hofstetter ◽  
Raj Murugesan
Keyword(s):  
Limit Of Detection ◽  
Animal Health ◽  
Contamination Level ◽  
Negative Effects ◽  
Mycotoxin Contamination ◽  
Contamination Levels ◽  
And Performance ◽  
Harvest Year ◽  
By Products ◽  
Corn Grain

Abstract Mycotoxins are fungal metabolites that limit animal health and performance through various negative effects including reduced feed intake, diarrhea, and compromised immune function. These toxic metabolites have been detected in a broad range of feedstuffs worldwide. This study investigated mycotoxin occurrence and contamination levels in U.S. corn grain samples marked as 2020 harvest year and corn by-product feed samples submitted since October 2020. Samples were screened via LC-MS/MS for six major mycotoxin groups including: aflatoxins, type A trichothecenes, type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN), and ochratoxin A. Results from samples that were above the limit of detection for each respective mycotoxin group were analyzed using the GLIMMIX procedure (SAS 9.4, Cary, NC) to test the effect of harvest year on mean contamination level. Results are presented in Table 1. Occurrence levels of B-Trich, ZEN, and FUM in 2020 corn grain are numerically lower than those from 2019. Mean B-Trich level (parts per billion, ppb) for corn grain samples harvested in 2020 was similar to 2019 crop year. Contamination levels of FUM and ZEN were similar for 2020 crop as compared to prior harvest years. Mycotoxin occurrence in corn by-product feeds continued to be high at 99, 85, and 95% for B-Trich, ZEN, and FUM, respectively. Mean B-Trich and ZEN levels in 2020 corn by-products were similar to respective contamination levels in 2018, but were lower than 2019 samples. Mean FUM contamination in corn by-products has been similar across reported years. Preliminary results of the 2020 corn grain survey indicate mean mycotoxin contamination levels are similar to those observed the past two crop years while levels of B-Trich and ZEN in corn by-product feeds are reduced from 2019. Corn-based feed ingredients may present challenges to producers due to the presence of mycotoxin contamination, therefore, continued monitoring is warranted.

136 Mycotoxin Contamination in United States Corn and Corn DDGS from 2019 and 2020 Harvest

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 73-74
Author(s):  
Lan Zheng ◽  
Chasity Pender ◽  
Paige N Gott ◽  
Erin F Schwandt ◽  
Shelby M Ramirez ◽  
...  
Keyword(s):  
Disease Incidence ◽  
Animal Health ◽  
Clinical Signs ◽  
Mitigation Strategies ◽  
Experimental Unit ◽  
Contamination Level ◽  
Mycotoxin Contamination ◽  
Contamination Levels ◽  
Harvest Year ◽  
Corn Ddgs

Abstract Mycotoxins are harmful secondary fungal metabolites central to food and feed safety management. These toxins are detrimental to animal health and even at low levels can compromise performance. Focus on clinical signs like decreased feed intake and vomiting overlook significant impacts of mycotoxicosis, including increased disease incidence and severity, immune dysfunction, inflammation, and modulation of the gastrointestinal environment. The objective of this study was to determine the effect of harvest year on five major mycotoxin groups: aflatoxins (Afla), type A trichothecenes (A-Trich), type B trichothecenes (B-Trich), fumonisins (FUM), and zearalenone (ZEN) in corn and corn DDGS samples. For each mycotoxin group within ingredient, 2019 harvest (329 corn and 20 corn DDGS) were compared with 2020 harvest (27 corn and 21 corn DDGS). Data were analyzed using GLIMMIX procedure of SAS with harvest year as fixed effect and sample as the experimental unit. Average B-Trich and FUM contamination levels in corn are significantly (P &lt; 0.05) affected by harvest year. However, B-Trich levels remained consistent, whereas FUM decreased (P &lt; 0.05) from 2019 to 2020. Contamination levels in corn for Afla, A-Trich, and ZEN have remained consistent (P &gt; 0.05) from 2019 to 2020. In corn DDGS, B-Trich contamination level was decreased (P &lt; 0.05) from 2019 to 2020, whereas FUM and ZEN contamination levels remained similar (P &gt; 0.05). The 2020 crop risk profile is likely to change as the sample pool expands. A combination of hot weather, storm events, and drought during the 2020 growing season resulted in crop stress and damage, ultimately leading to grain quality and mycotoxin contamination concerns. Due to the continued risk of mycotoxin co-occurrence, expanding mitigation strategies beyond adsorption by mycotoxin deactivation with biotransformation and additional support of immune and liver function is essential.

Safety of low and high cost dry feed intended for dogs in Brazil concerning fumonisins, zearalenone and aflatoxins

2017 ◽  
Vol 10 (3) ◽  
pp. 273-283 ◽  
Author(s):  
E.M.K. Teixeira ◽  
M.S. Frehse ◽  
R.L. Freire ◽  
M.A. Ono ◽  
J.G. Bordini ◽  
...  
Keyword(s):  
Low Cost ◽  
Animal Health ◽  
Daily Intake ◽  
Estimated Daily Intake ◽  
Commercial Feed ◽  
Negative Effect ◽  
Contamination Levels ◽  
And Performance ◽  
Aflatoxin B ◽  
Feed Samples

The Brazilian pet sector exportation has been growing at an impressive rate lately. Considering the intense globalisation, pet food may be commercialised worldwide and the issue concerning safety of dry dog feed has become a topic of widespread international interest. Therefore, the objectives of this study were to evaluate the safety of low and high cost dry feed and the degree of exposure of dogs to mycotoxins through naturally contaminated feed in Brazil. In addition, the safe pet dietary level (SPDL) was calculated. Fumonisins, zearalenone (ZEA) and aflatoxins were analysed in three feed types intended for dogs (n=87), provided by pet owners. Mean fumonisin levels were 272 µg/kg (Standard), 78.2 µg/kg (Premium), and 186 µg/kg (Super Premium) while ZEA mean levels were 52.6 µg/kg (Standard), 10.6 µg/kg (Premium) and 17.5 µg/kg (Super Premium). Mean total aflatoxin levels (aflatoxin B1 + B2 + G1 + G2) in Standard, Premium and Super Premium feed were 1.29 µg/kg, 0.49 µg/kg and 0.53 µg/kg, respectively. The estimated daily intake (EDI) values for fumonisin B1 were 1.41 (Standard), 1.60 (Premium) and 0.93 µg/kg body weight (BW)/day (Super Premium feed), whereas for ZEA they were 0.54 (Standard), 0.37 (Premium) and 0.42 µg/kg BW/day (Super Premium feed). SPDL for fumonisins and ZEA was 2,000 and 375 µg/kg feed, respectively. EDI values for aflatoxin B1 were 13.0 (Standard), 14.0 (Premium) and 11.0 ng/kg BW/day (Super Premium feed). Although the feed samples intended for dogs in Brazil were often contaminated with aflatoxins, fumonisins and ZEA, the estimated daily intake values were low, even for the low cost formulations. However, it cannot be excluded that some of the feed samples could have a negative effect on animal health and performance considering feeding a monodiet of commercial feed, low multi-mycotoxin contamination levels and interactions between mycotoxins.

Management of mycotoxin contamination in food and feed in China

2014 ◽  
Vol 7 (1) ◽  
pp. 53-62 ◽  
Author(s):  
W.W. Zhang ◽  
Z.M. Ye ◽  
Y. Jin ◽  
S.Y. Wang ◽  
L.S. Zhang ◽  
...  
Keyword(s):  
Safety Management ◽  
Animal Feed ◽  
Animal Health ◽  
Management Strategies ◽  
Monitoring Network ◽  
Self Control ◽  
Risk Assessments ◽  
Mycotoxin Contamination ◽  
Code Of Practice ◽  
Food And Feed

Mycotoxins have a significant impact on human and animal health as well as economy and international trade. A number of steps have been taken to minimise mycotoxin contamination worldwide. China, one of the largest importers and exporters of food and animal feed, has implemented management strategies to protect consumers from mycotoxins and ensure safe trading of food and feed. The development and status of these management strategies are of interest to many stakeholders, however, no comprehensive review of these strategies has been published to date. The objective of this article is to review these strategies, including associated regulations, risk assessments, contamination monitoring practices, and processes for the supervision and management of associated production, distribution and consumption chains. There are 49 mycotoxin-related regulations in China; these include maximum levels for seven mycotoxins, standard methods for detecting 17 mycotoxins, and a code of practice for the prevention and reduction of mycotoxin contamination in cereals. Twelve mycotoxins found in daily food are covered by the Chinese contamination monitoring network. A new risk assessment organisation is required to systematically implement and manage food safety risk assessments in China. In addition, self-control by companies and daily supervisions and inspections by government departments are quite effective in preventing the consumption of mycotoxin-contaminated food and feed. This article provides information to all those who wish to understand more about the current safety management systems for combating mycotoxin contamination of food and feed in China.

Mycotoxins in silage: checkpoints for effective management and control

2015 ◽  
Vol 8 (5) ◽  
pp. 603-617 ◽  
Author(s):  
V. Dell’Orto ◽  
G. Baldi ◽  
F. Cheli
Keyword(s):  
Animal Feed ◽  
Negative Impact ◽  
Synergistic Effects ◽  
Animal Health ◽  
Fungal Growth ◽  
Control Programme ◽  
Production Chain ◽  
Mycotoxin Contamination ◽  
Post Harvest ◽  
And Control

Silage has a substantial role in ruminant nutrition. Silages as a source of mycotoxigenic fungi and mycotoxins merit attention. Fungal growth and mycotoxin production before and during storage are a well-known phenomenon, resulting in reduced nutritional value and a possible risk factor for animal health. Mycotoxin co-contamination seems to be unavoidable under current agricultural and silage-making practices. Multi-mycotoxin contamination in silages is of particular concern due to the potential additive or synergistic effects on animals. In regard to managing the challenge of mycotoxins in silages, there are many factors with pre- and post-harvest origins to take into account. Pre-harvest events are predominantly dictated by environmental factors, whereas post-harvest events can be largely controlled by the farmer. An effective mycotoxin management and control programme should be integrated and personalised to each farm at an integrative level throughout the silage production chain. Growing crops in the field, silage making practices, and the feed out phase must be considered. Economical and straightforward silage testing is critical to reach a quick and sufficiently accurate diagnosis of silage quality, which allows for ‘in field decision-making’ with regard to the rapid diagnosis of the quality of given forage for its safe use as animal feed. Regular sampling and testing of silage allow picking up any variations in mycotoxin contamination. The use of rapid methods in the field represents future challenges. Moreover, a proper nutritional intervention needs to be considered to manage mycotoxin-contaminated silages. At farm level, animals are more often exposed to moderate amounts of several mycotoxins rather than to high levels of a single mycotoxin, resulting more frequently in non-specific digestive and health status impairment. Effective dietary strategies to promote rumen health, coupled with the administration of effective and broad-spectrum mycotoxin detoxifiers, are essential to minimise the negative impact of mycotoxins.

Study on mycotoxin contamination in South African food spices

2018 ◽  
Vol 11 (3) ◽  
pp. 401-409 ◽  
Author(s):  
L. Motloung ◽  
S. De Saeger ◽  
M. De Boevre ◽  
C. Detavernier ◽  
K. Audenaert ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
South African ◽  
Ochratoxin A ◽  
Tandem Mass ◽  
Mycotoxin Contamination ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Contamination Levels ◽  
Aflatoxin B ◽  
Roquefortine C

A validated QuEChERS-based method was used to investigate the occurrence of mycotoxins in 70 South African food spices [coarse chilli (n=14), ground chilli (n=4), paprika (n=7), ginger (n=5), chicken spices (n=8), onion spices (n=8), beef spices (n=5), Mexican chilli (n=9), vegetable spice (n=1), fruit chutney spices (n=4), and cheese spices (n=5)]. Mycotoxins were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results revealed that 40% of the samples were contaminated with aflatoxin B1, aflatoxin G1, ochratoxin A, sterigmatocystin, 3-acetyldeoxynivalenol, fumonisin B1, fumonisin B2 and/or roquefortine C. The contamination levels for aflatoxin B1 ranged from 3-19 μg/kg; aflatoxin G1, 10-11 μg/kg; ochratoxin A, 4-20 μg/kg; fumonisin B1 104-591 μg/kg; fumonisin B2, 64-5,897 μg/kg; sterigmatocystin, 11-18 μg/kg; 3-acetyldeoxynivalenol, 42-46 μg/kg; and roquefortine C, 17-57 μg/kg. Mycotoxins co-occurred in 11% of the spice samples. Amongst the samples analysed in this study, paprika had the highest positives (100%) for the determined mycotoxins. Previous reports on mycotoxin contamination in spices, focused on the incidence of aflatoxins and ochratoxin A, but not on the multi-mycotoxin profile in South African spices. This study thus provides a comprehensive assessment of mycotoxin contamination of spices in South Africa.

scholarly journals Clostridia in Insect Processed Animal Proteins—Is an Epidemiological Problem Possible?

Agriculture ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 270
Author(s):  
Tomasz Grenda ◽  
Krzysztof Kwiatek ◽  
Magdalena Goldsztejn ◽  
Magdalena Sapała ◽  
Nina Kozieł ◽  
...  
Keyword(s):  
16S Rdna ◽  
Animal Feed ◽  
Statistical Significance ◽  
Contamination Level ◽  
Type D ◽  
Botulinum Type ◽  
Livestock Animal ◽  
Contamination Levels ◽  
Rdna Analysis ◽  
Clostridium Spp

The aim of this study was the evaluation of the insect processed animal protein (IPAP) contamination level by Clostridium spp. Particularly, we screened for the occurrence of pathogenic species of Clostridia. The samples of IPAP were derived from yellow mealworm (Tenebrio molitor) and black soldier fly (Hermetia illucens) available in the Polish market. The IPAPs were added to experimental feeds for poultry. The differences between the contamination levels of the control (without the addition of IPAP) and experimental (with the addition of IPAP) groups were monitored. The samples were also examined by culture and PCR-based methods to detect 16S rDNA and genes determining botulinum toxin (BoNT) production. Statistical significance was noticed among the feed with the IPAP addition, as well as an increase of contamination by Clostridium spp. In one sample of IPAP, the occurrence of ntnh and bont/D genes determining the production of BoNT/D was noticed. However, a positive result was noticed only at the step of the liquid culture; the Clostridium botulinum type D strain was not isolated. Phenotypically, and according to the 16S rDNA analysis, genetically similar strains to C. botulinum species were isolated. Considering the microbiological safety of IPAP and expanding possibility of its use in livestock animal feed, it seems to be reasonable to provide complex risk assessment on the potential transfer of Clostridia into feed compounds, to assure the safety and sustainable development of insect PAP industry.

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