The use of LC-Orbitrap MS for the detection of Fusarium masked mycotoxins: the case of type A trichothecenes

2012 ◽  
Vol 5 (3) ◽  
pp. 271-280 ◽  
Author(s):  
H. Nakagawa ◽  
S. Sakamoto ◽  
Y. Sago ◽  
M. Kushiro ◽  
H. Nagashima
Keyword(s):  
Type A ◽  
Mass Spectrometric ◽  
Molar Ratio ◽  
Spectrometric Analysis ◽  
Mass Measurements ◽  
Masked Mycotoxins ◽  
Accurate Mass Measurements ◽  
Type A Trichothecenes ◽  
Fragmentation Patterns ◽  
Orbitrap Ms

‘Masked mycotoxins’ (mycotoxin glucosides) derived from type A trichothecenes (T-2 toxin and HT-2 toxin) were detected in commercially available maize powder reference material. These novel glucosides were identified as T-2 toxin-glucoside (T2-Glc) and HT-2 toxin-glucoside (HT2-Glc) on the basis of accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography Orbitrap mass spectrometric analysis. Although their absolute structures were not clarified, 3-OH glucosylation appeared to be the most probable when considering the structure of T-2 toxin and the fragment profiles of each masked mycotoxin. Concomitant detection of deoxynivalenol-3-glucoside (DON-3G) in the same material further supports this probability. According to an extrapolation based on the molar ratio DON-3G/DON (0.059), amounts of T2-Glc and HT2-Glc were estimated to be approximately 24 μg/kg and 41 μg/kg, respectively.

Some Aspects of the Mass Spectra of.N-Nitrosamines

1971 ◽  
Vol 49 (9) ◽  
pp. 1367-1371 ◽  
Author(s):  
J. W. ApSimon ◽  
J. D. Cooney
Keyword(s):  
Mass Spectra ◽  
Accurate Mass ◽  
Mass Measurements ◽  
Molecular Ion ◽  
Accurate Mass Measurements ◽  
Characteristic Fragmentation ◽  
Fragmentation Patterns

The mass spectra of seven cyclic N-nitrosamines were examined for characteristic fragmentation patterns. Accurate mass measurements on three of the compounds indicated that the M-17 and M-30 peaks resulted from molecular ion losses of •OH and •NO respectively. The loss of • OH was rationalized in terms of a McLafferty-type rearrangement.

scholarly journals Gas Chromatographic and Mass Spectrometric Analysis of Nitrilotriacetic Acid in Environmental Aqueous Samples

2002 ◽  
Vol 85 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Asit Raksit
Keyword(s):  
Accurate Method ◽  
Nitrilotriacetic Acid ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Aqueous Samples ◽  
Gas Chromatograph ◽  
Method Detection Limit ◽  
Mass Spectrometric Detector ◽  
Fragmentation Patterns ◽  
Acid Esters

Abstract This study describes a fast and accurate method for the sample preparation, identification, and quantitation of nitrilotriacetic (NTA) acid in environmental aqueous samples at a concentration of ppb level. The method is sensitive, specific, and free from the interferences of fatty and amino acids. The tri-n-propyl- and tri-n-butyl-NTA acid esters were prepared by the reaction of n-propyl-HCl and n-butyl-HCl solutions and NTA acid, respectively. The derivatives were analyzed by a gas chromatograph equipped with a mass spectrometric detector. The method detection limit, 0.006 mg/L of each NTA ester, was determined and validated by an analysis of a fortified water sample. The overall recoveries were 103–115%, n = 8. The method was applied to a real sample and a 0.90 mg/L concentration of NTA acid was found. Mass fragmentation patterns of the derivatives are also reported.

scholarly journals Rapid Mass Spectrometric Analysis of a Novel Fucoidan, Extracted from the Brown AlgaCoccophora langsdorfii

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Stanislav D. Anastyuk ◽  
Tatyana I. Imbs ◽  
Pavel S. Dmitrenok ◽  
Tatyana N. Zvyagintseva
Keyword(s):  
Mass Spectra ◽  
Structural Features ◽  
Degree Of Polymerization ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Low Molecular Weight ◽  
Ionization Mass ◽  
The Novel ◽  
Fragmentation Patterns ◽  
Rapid Mass

The novel highly sulfated (35%) fucoidan fractionCf2, which contained, along with fucose, galactose and traces of xylose and uronic acids was purified from the brown algaCoccophora langsdorfii. Its structural features were predominantly determined (in comparison with fragments of known structure) by a rapid mass spectrometric investigation of the low-molecular-weight fragments, obtained by “mild” (5 mg/mL) and “exhaustive” (maximal concentration) autohydrolysis. Tandem matrix-assisted laser desorption/ionization mass spectra (MALDI-TOF/TOFMS) of fucooligosaccharides with even degree of polymerization (DP), obtained by “mild” autohydrolysis, were the same as that observed for fucoidan fromFucus evanescens, which have a backbone of alternating (1 → 3)- and (1 → 4) linked sulfated at C-2 and sometimes at C-4 of 3-linkedα-L-Fucpresidues. Fragmentation patterns of oligosaccharides with odd DP indicated sulfation at C-2 and at C-4 of (1 → 3) linkedα-L-Fucpresidues on the reducing terminus. Minor sulfation at C-3 was also suggested. The “exhaustive” autohydrolysis allowed us to observe the “mixed” oligosaccharides, built up of fucose/xylose and fucose/galactose. Xylose residues were found to occupy both the reducing and nonreducing termini of FucXyl disaccharides. Nonreducing galactose residues as part of GalFuc disaccharides were found to be linked, possibly, by 2-type of linkage to fucose residues and were found to be sulfated, most likely, at position C-2.

scholarly journals Benzodiazepine Analogues. Part 20. Mass Spectrometric Analysis of Benzoxathiepine Derivatives

1999 ◽  
Vol 23 (9) ◽  
pp. 584-585
Author(s):  
Aifheli C. Gelebe ◽  
Perry T. Kaye
Keyword(s):  
High Resolution ◽  
Electron Impact ◽  
Mass Spectra ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Low Resolution ◽  
Fragmentation Patterns ◽  
Resolution Mass

Fragmentation patterns in the electron-impact (El) mass spectra of benzoxathiepine derivatives are elucidated using a combination of high-resolution and comparative low-resolution mass spectrometric analysis.

Chromone Studies. Part 11.1 Synthesis and Electron-Impact Mass Spectrometric Study of Granulosin and Side-Chain Analogues

2002 ◽  
Vol 2002 (7) ◽  
pp. 321-325 ◽  
Author(s):  
Perry T. Kaye ◽  
Aloysius T. Nchinda ◽  
Christopher A. Gray
Keyword(s):  
Electron Impact ◽  
Mass Spectrometric Study ◽  
Artemia Salina ◽  
Mass Spectrometric ◽  
Side Chain ◽  
Spectrometric Analysis ◽  
Electron Impact Mass ◽  
Impact Ionisation ◽  
Fragmentation Patterns ◽  
Impact Mass

Granulosin, a chromone constituent of the bark of Galipea granulosa, and four analogues, all of which exhibit toxicity to the brine shrimp Artemia salina, have been prepared from 2′,3′,4′-trihydroxyacetophenone. High-resolution mass spectrometric analysis has permitted elucidation of the fragmentation patterns exhibited by these systems following electron-impact ionisation.

A Questionnaire Survey on General Status and Opinions about Clinical Mass Spectrometric Analysis in Korea (2018)

2019 ◽  
Vol 9 (3) ◽  
pp. 161
Author(s):  
Sung-Eun Cho ◽  
Hyojin Chae ◽  
Hyung-Doo Park ◽  
Sail Chun ◽  
Yong-Wha Lee ◽  
...  
Keyword(s):  
Questionnaire Survey ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
General Status
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