scholarly journals Rapid Mass Spectrometric Analysis of a Novel Fucoidan, Extracted from the Brown AlgaCoccophora langsdorfii

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Stanislav D. Anastyuk ◽  
Tatyana I. Imbs ◽  
Pavel S. Dmitrenok ◽  
Tatyana N. Zvyagintseva
Keyword(s):  
Mass Spectra ◽  
Structural Features ◽  
Degree Of Polymerization ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Low Molecular Weight ◽  
Ionization Mass ◽  
The Novel ◽  
Fragmentation Patterns ◽  
Rapid Mass

The novel highly sulfated (35%) fucoidan fractionCf2, which contained, along with fucose, galactose and traces of xylose and uronic acids was purified from the brown algaCoccophora langsdorfii. Its structural features were predominantly determined (in comparison with fragments of known structure) by a rapid mass spectrometric investigation of the low-molecular-weight fragments, obtained by “mild” (5 mg/mL) and “exhaustive” (maximal concentration) autohydrolysis. Tandem matrix-assisted laser desorption/ionization mass spectra (MALDI-TOF/TOFMS) of fucooligosaccharides with even degree of polymerization (DP), obtained by “mild” autohydrolysis, were the same as that observed for fucoidan fromFucus evanescens, which have a backbone of alternating (1 → 3)- and (1 → 4) linked sulfated at C-2 and sometimes at C-4 of 3-linkedα-L-Fucpresidues. Fragmentation patterns of oligosaccharides with odd DP indicated sulfation at C-2 and at C-4 of (1 → 3) linkedα-L-Fucpresidues on the reducing terminus. Minor sulfation at C-3 was also suggested. The “exhaustive” autohydrolysis allowed us to observe the “mixed” oligosaccharides, built up of fucose/xylose and fucose/galactose. Xylose residues were found to occupy both the reducing and nonreducing termini of FucXyl disaccharides. Nonreducing galactose residues as part of GalFuc disaccharides were found to be linked, possibly, by 2-type of linkage to fucose residues and were found to be sulfated, most likely, at position C-2.

Direct analysis of herbicides by paper spray ionization mass spectrometry

2015 ◽  
Vol 7 (23) ◽  
pp. 9808-9816 ◽  
Author(s):  
Steven L. Reeber ◽  
Sneha Gadi ◽  
Sung-Ben Huang ◽  
Gary L. Glish
Keyword(s):  
Mass Spectrometry ◽  
Environmental Samples ◽  
Direct Analysis ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Paper Spray ◽  
Paper Spray Ionization ◽  
Rapid Mass

Paper spray ionization enables the rapid mass spectrometric analysis of environmental samples without the use of chromatography or sample cleanup techniques.

Piezoelectric inkjet assisted rapid electrospray ionization mass spectrometric analysis of metabolites in plant single cells via a direct sampling probe

The Analyst ◽  
2014 ◽  
Vol 139 (22) ◽  
pp. 5734-5739 ◽  
Author(s):  
Zhan Yu ◽  
Lee Chuin Chen ◽  
Satoshi Ninomiya ◽  
Mridul Kanti Mandal ◽  
Kenzo Hiraoka ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Single Cells ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Direct Sampling ◽  
Sampling Probe ◽  
Surface Modified ◽  
Rapid Mass

We report the combination of a piezoelectric inkjet and a surface modified sharp tipped probe for the direct sampling and rapid mass spectrometric analysis of metabolites in plant single cells.

Gas Chromatography Electron Ionization Mass Spectrometric Analysis of O-Alkyl Methylphosphinates for Verification of Chemical Weapons Convention

2009 ◽  
Vol 15 (5) ◽  
pp. 579-585 ◽  
Author(s):  
Deepak Pardasani ◽  
Pankaj K. Kanaujia ◽  
Vijay Tak ◽  
Prabhat Garg ◽  
Avik Mazumder ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Mass Spectra ◽  
Electron Ionization ◽  
Mass Spectrometric ◽  
Chemical Weapons ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Ms Analysis ◽  
Chemical Weapons Convention ◽  
Hydrogen Rearrangement

We describe the gas chromatography/mass spectrometric (GC/MS) analysis of O-alkyl methylphosphinates (AMPs), which are included in Schedule 2B4 chemicals in the Chemical Weapons Convention (CWC). GC/MS analysis of a variety of AMPs and their deuterated analogs revealed that their fragmentations were determined by α-cleavages, McLafferty +1 and hydrogen rearrangement. Based on the obtained electron ionization mass spectra of AMPs, the fragmentation routes were rationalized, which were substantiated by the GC/MS analysis of deuterated analogs.

scholarly journals Benzodiazepine Analogues. Part 20. Mass Spectrometric Analysis of Benzoxathiepine Derivatives

1999 ◽  
Vol 23 (9) ◽  
pp. 584-585
Author(s):  
Aifheli C. Gelebe ◽  
Perry T. Kaye
Keyword(s):  
High Resolution ◽  
Electron Impact ◽  
Mass Spectra ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Low Resolution ◽  
Fragmentation Patterns ◽  
Resolution Mass

Fragmentation patterns in the electron-impact (El) mass spectra of benzoxathiepine derivatives are elucidated using a combination of high-resolution and comparative low-resolution mass spectrometric analysis.

scholarly journals Overcharging Effect in Electrospray Ionization Mass Spectra of Daunomycin-Tuftsin Bioconjugates

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2981
Author(s):  
Lilla Pethő ◽  
Gábor Mező ◽  
Gitta Schlosser
Keyword(s):  
Electrospray Ionization ◽  
Mass Spectra ◽  
Source Parameters ◽  
Tumor Therapy ◽  
Ion Source ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Drug Molecules ◽  
Drug Conjugates

Peptide-based small molecule drug conjugates for targeted tumor therapy are currently in the focus of intensive research. Anthracyclines, like daunomycin, are commonly used anticancer drug molecules and are also often applied in peptide-drug conjugates. However, lability of the O-glycosidic bond during electrospray ionization mass spectrometric analysis hinders the analytical characterization of the constructs. “Overprotonation” can occur if daunomycin is linked to positively charged peptide carriers, like tuftsin derivatives. In these molecules, the high number of positive charges enhances the in-source fragmentation significantly, leading to complex mass spectra composed of mainly fragment ions. Therefore, we investigated different novel tuftsin-daunomycin conjugates to find an appropriate condition for mass spectrometric detection. Our results showed that shifting the charge states to lower charges helped to keep ions intact. In this way, a clear spectrum could be obtained containing intact protonated molecules only. Shifting of the protonation states to lower charges could be achieved with the use of appropriate neutral volatile buffers and with tuning the ion source parameters.

Electrospray ionization mass spectrometric analysis of phospholipids of Escherichia coli

1996 ◽  
Vol 20 (1) ◽  
pp. 233-238 ◽  
Author(s):  
G. Sweetman ◽  
M. Trinei ◽  
J. Modha ◽  
J. Kusel ◽  
P. Freestone ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Electrospray Ionization ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Ionization Mass

scholarly journals Rapid Mass Spectrometric Diagnosis of Bladder Cancer via Urine Carbonyl Metabolic Fingerprints

2021 ◽  
Author(s):  
Zongxiu Nie ◽  
Yuze Li ◽  
Lixia Jiang ◽  
Zhenpeng Wang ◽  
Xiaohua Cao ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Point Of Care ◽  
Low Cost ◽  
Mass Spectrometric ◽  
Detection Sensitivity ◽  
Ionization Mass ◽  
Non Invasive ◽  
Diagnosis Method ◽  
Rapid Mass ◽  
Potential Biomarkers

Abstract The diagnosis of bladder cancer (BC) is currently based on cystoscopy, which is invasive and expensive. Here, we described a non-invasive, low-cost BC diagnosis method based on a desorption, separation, and ionization mass spectrometry platform (DSI-MS) that adopts N, N- Dimethylethylenediamine (DMED) as a differential labeling reagent. The DSI-MS platform avoids the interferences from intra- and/or inter-samples, while the DMED increases detection sensitivity and distinguishes carboxyl, aldehyde, and ketone groups from untreated samples. Carbonyl metabolic fingerprints of urine from 28 BC patients and 38 controls were portrayed and significant differences of some potential biomarkers were observed. The mechanisms of the changes have been discussed. Logistic regression (LR) was applied to discriminate BC from controls and an accuracy of 87% was achieved. We believe this patient-friendly method provides a hopeful approach for BC rapid point-of-care diagnostic.

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