scholarly journals The effect of Rhodococcus rhodochrous supplementation on black soldier fly (Diptera: Stratiomyidae) development, nutrition, and waste conversion

2021 ◽  
pp. 1-12
Author(s):  
K. Franks ◽  
E. Kooienga ◽  
M. Sanders ◽  
K. Pendarvis ◽  
F. Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Stress Responses ◽  
Muscle Development ◽  
Protein Composition ◽  
Rhodococcus Rhodochrous ◽  
Larval Body ◽  
Black Soldier Fly ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae ◽  
Waste Conversion

Black soldier fly larvae are mass produced globally for use as livestock, poultry, and aquaculture feed. Efforts are continuously seeking processes optimising larval rate of growth, size, and waste conversion as a means to lower cost and increase output. Manipulating microbes in the larval substrate (i.e. fermentation or supplementation) has been demonstrated as a potential solution. However, identifying appropriate microbes for use in this process has been limited. The objective of this study was to determine whether supplementing black soldier fly larvae with the oleaginous microbe Rhodococcus rhodochrous would result in accelerated larval development, increased final larval body size, and increased conversion efficiency. Larvae fed a sterile, or non-sterile, diet treated with R. rhodochrous grew 3× faster than the control by the third day and were approximately 2× larger than the control by the conclusion of the experiment. Conversion rate was 2× greater for the treatments indicating less feed would be needed to achieve maximum weight gain. Protein composition of resulting larvae fed diet supplemented with R. rhodochrous, was significantly different than the control not receiving the microbial infusion. Larvae provided the microbe had 4.20 and 2.79% greater fatty acid composition and short-chained fatty acids, respectively, but lower monounsaturated fatty acids (1.60%) and polyunsaturated fatty acids (2.4%). Furthermore, larvae provided R. rhodochrous produced significantly more proteins related to energy production and storage, as well as muscle development and contraction, while those sans microbe, produced proteins related to stress responses (e.g. heat shock proteins). While, this study yielded positive results for the inclusion of R. rhodochrous as part of the black soldier fly larval diet, additional research is needed to optimise the dose at an industrial scale.

Enrichment of polyunsaturated fatty acids in black soldier fly larvae (Hermetia illucens) fortified with squid liver oil

2021 ◽  
pp. 1-12
Author(s):  
G. Tirtawijaya ◽  
J.-S. Choi
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Animal Feed ◽  
Control Group ◽  
Feed Conversion ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Growth Increase ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

Black soldier fly larvae (BSFL; Hermetia illucens) are known as an alternative feed for livestock, but their lack of polyunsaturated fatty acids (such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) makes it less valuable. To overcome these problems, BSFL substrate (chicken feed) was fortified with squid liver oil (SLO) at five different concentrations (0, 2.5, 5, 10, and 20%). The growth rate, feed conversion, and nutritional content of BSFL were evaluated at day 15 of rearing. Of the five concentrations, SLO 5% showed the highest growth increase (25.82-fold) among the other treatments (20.63-22.98-fold; P<0.05). The fortification of SLO 5% did not result in differences in survival and feed conversion of larvae compared to larvae fed the control substrate. By rearing BSFL in a substrate containing SLO 5%, the lipid content of the larvae was 32% higher than that of larvae fed the control substrate (P<0.05). The accumulation of lipids was faster in the substrate containing SLO 5% (33.20% for 8 days) than in the control substrate (24.36% for 15 days). The control group of BSFL contained no DHA or EPA, but after rearing on the SLO 5% fortified substrate, the larvae contained DHA at an average level of 2.99 g/100 g lipid and EPA was 2.68 g/100 g lipid. Harvested larvae from SLO 5% treatment was within safe levels of Pb, Cd, As, and Hg (840, 370, 860, and 26.7 μg/kg, respectively), under the EU threshold for animal feed. Based on our results, it concluded that BSFL enriched with PUFAs, DHA and EPA can be considered as important nutritional components of animal feed without excessive heavy metals accumulation by feeding SLO in an appropriate amount.

scholarly journals Novel Insights on the Sustainable Wet Mode Fractionation of Black Soldier Fly Larvae (Hermetia illucens) into Lipids, Proteins and Chitin

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1888
Author(s):  
Harish Karthikeyan Ravi ◽  
Côme Guidou ◽  
Jérôme Costil ◽  
Christophe Trespeuch ◽  
Farid Chemat ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Press Cake ◽  
Amino Acid Profile ◽  
Subsequent Treatment ◽  
Total Energy Consumption ◽  
Black Soldier Fly ◽  
Aqueous Fraction ◽  
Unit Operations ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

The black soldier fly larvae (BSFL) is a sustainable ingredient for feed applications, biofuels, composite materials and other biobased products. Processing BSFL to obtain lipid and protein fractions with enhanced functional properties as a suitable replacement for conventional feed ingredients has gained considerable momentum. In this regard, a novel and sustainable wet mode fractionation (WMF) scheme for BSFL was explored. Fresh BSFL were steam blanched and pulped to obtain BSFL juice and juice press cake. Subsequent treatment of BSFL juice employing homogenization or enzyme incubation and further centrifugation resulted in the obtention of four different BSFL fractions (Lipid—LF; Cream—CF; Aqueous—AF; and Solid—SF). Total energy consumption for a batch BSFL (500 g) WMF process was 0.321 kWh. Aqueous and solid fractions were the predominant constituents of BSFL juice. Lauric acid (44.52–49.49%) and linoleic acid (19.12–20.12%) were the primary fatty acids present in BSFL lipids. Lipid hydrolysis was observed in lipids belonging to the solid (free fatty acids > triacylglycerides) and cream fractions. Aqueous fraction proteins (ctrl) displayed superior emulsion stability and foam capacity than other treatments. Juice press cake retained 60% of the total chitin content and the rest, 40%, was found in the solid fraction (ctrl). The material distribution of principal constituents in different fractions of the WMF process and amino acid profile was elucidated. Overall, the versatile WMF process proposed in this study involves simple unit operations to obtain functional ingredients from BSFL, which can be further explored by researchers and industry stakeholders.

Dose-Dependent Retention of Omega-3 Fatty Acids by Black Soldier Fly Larvae (Diptera: Stratiomyidae)

2020 ◽  
Vol 113 (3) ◽  
pp. 1221-1226
Author(s):  
Patrick Erbland ◽  
Andrei Alyokhin ◽  
L Brian Perkins ◽  
Michael Peterson
Keyword(s):  
Fatty Acids ◽  
Individual Growth ◽  
Dependent Manner ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Alpha Linolenic Acid ◽  
Black Soldier Fly ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae ◽  
Dose Dependent

Abstract Black soldier fly larvae, Hermetia illucens (L.), are used to convert organic waste streams into insect-based animal feeds. We tested their ability to retain alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) from feeding substrates, which has important implications for their use in aquaculture. When supplementing a chicken feed diet with increasing concentrations of salmon oil (0–42%) over an increasing number of days (0–8), the concentrations of the three omega-3 acids in larvae increased significantly. Larval survival and biomass accumulation were not affected. Supplementing a chicken feed diet with increasing concentrations (0–14%) of Tetraselmis chui Butcher (Chlorodendrales: Chlorodendraceae) microalgae paste also significantly increased ALA and EPA contents of the harvested larvae. However, microalgae also decreased survival, harvested biomass, and individual growth of larvae feeding on the diet with the highest supplement concentration (14%). DHA was not detected in any microalgae diet or subsequent larval tissue samples. All three omega-3 polyunsaturated fatty acids tested in this study were accumulated in dose-dependent manner, with quadratic, and occasionally linear, equations providing the best description of the observed relationships. There were significant negative correlations between several fatty acids, indicating that they may replace one another in living larvae. Our findings confirm that black soldier fly larvae can retain ingested fatty acids and change fatty acid profiles in their tissues accordingly. However, optimizing nutrient content of harvestable larvae is likely to be more complicated than simply enriching their diets with omega-3 fatty acids.

scholarly journals Impact of black soldier fly larvae meal on the chemical and nutritional characteristics of rainbow trout fillets

animal ◽  
2018 ◽  
Vol 12 (8) ◽  
pp. 1672-1681 ◽  
Author(s):  
S. Mancini ◽  
I. Medina ◽  
V. Iaconisi ◽  
F. Gai ◽  
A. Basto ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Black Soldier Fly ◽  
Nutritional Characteristics ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

scholarly journals Black Soldier Fly larvae meal and fat can completely replace soybean cake and oil in diets for laying hens

2021 ◽  
pp. 101034
Author(s):  
M. Heuel ◽  
C. Sandrock ◽  
F. Leiber ◽  
A. Mathys ◽  
M. Gold ◽  
...  
Keyword(s):  
Laying Hens ◽  
Black Soldier Fly ◽  
Soybean Cake ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

scholarly journals Feeding live Black Soldier Fly larvae (Hermetia illucens) to laying hens: effects on feed consumption, hen health, hen behaviour and egg quality

2021 ◽  
pp. 101400
Author(s):  
Fernanda M. Tahamtani ◽  
Emma Ivarsson ◽  
Viktoria Wiklicky ◽  
Cecilia Lalander ◽  
Helena Wall ◽  
...  
Keyword(s):  
Laying Hens ◽  
Egg Quality ◽  
Feed Consumption ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

scholarly journals Cost-Effectiveness of Black Soldier Fly Larvae Meal as Substitute of Fishmeal in Diets for Layer Chicks and Growers

2021 ◽  
Vol 13 (11) ◽  
pp. 6074
Author(s):  
Esther Khayanga Sumbule ◽  
Mary Kivali Ambula ◽  
Isaac Maina Osuga ◽  
Janice Ghemoh Changeh ◽  
David Miano Mwangi ◽  
...  
Keyword(s):  
Weight Gain ◽  
Live Weight ◽  
Daily Weight Gain ◽  
Feed Conversion ◽  
Alternative Source ◽  
Black Soldier Fly ◽  
Promising Alternative ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae ◽  
The Impact

The acceptance of eco-friendly black soldier fly larvae meal (BSFLM) as sustainable alternative protein ingredient in poultry feeds continues to gain momentum worldwide. This study evaluates the impact of BSFLM in layer chick and grower diets on the growth, carcass quality and economic returns. Mean weekly weight gain and total live weight per chick and grower varied significantly. The highest final weight gain was achieved when birds were provided diet with 25.6% BSFLM. Average daily feed intake (ADFI), average daily weight gain (ADG) and overall weight gain of the chick varied significantly, except for the feed conversion ratio (FCR). For grower birds, ADFI, ADG, FCR and overall weight gain did not vary significantly across the various feeding regimes. The weight of the wings and drumsticks had a quadratic response with a maximum weight obtained at 33% inclusion of BSFLM. The weight of the internal organs were not significantly affected by dietary types. Positive cost–benefit ratio and return on investment was recorded for diet types with higher BSFLM inclusion levels (>75%). Diets with 25% and 100% BSFLM inclusion were the most suitable and cost-effective, respectively. Thus, BSFLM represents a promising alternative source of protein that could be sustainably used in the poultry industries.

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