Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 excludes Listeria monocytogenes from the GIT, as shown by bioluminescent studies in mice

2016 ◽  
Vol 7 (2) ◽  
pp. 227-235 ◽  
Author(s):  
W.F. van Zyl ◽  
S.M. Deane ◽  
L.M.T. Dicks
Keyword(s):  
Listeria Monocytogenes ◽  
Lactobacillus Plantarum ◽  
Intestinal Tract ◽  
Competitive Exclusion ◽  
High Cell ◽  
Cell Numbers ◽  
Food Borne ◽  
Enterococcus Mundtii ◽  
Life Threatening ◽  
Gastro Intestinal Tract

Listeria monocytogenes is an opportunistic food-borne pathogen and is life-threatening to individuals with a weakened immune system. The aim of this study was to determine if Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA could prevent colonisation of L. monocytogenes in the gastro-intestinal tract (GIT). Mice were gavaged with L. plantarum 423, E. mundtii ST4SA, and a combination of the two strains, for 6 consecutive days and orally infected with a bioluminescent strain of L. monocytogenes (strain EGDe) on the last day of treatment. 30 min after infection, high cell numbers of L. plantarum 423, E. mundtii ST4SA and L. monocytogenes EGDe were isolated from faeces. L. monocytogenes EGDe cells were absent from the small intestine of L. plantarum 423-treated mice 4 h after infection and from the large intestine 2 h later. No bioluminescent, and thus metabolically active, cells of L. monocytogenes EGDe were recorded in the GIT of mice treated with E. mundtii ST4SA, suggesting that their growth was repressed. L. plantarum 423 and E. mundtii ST4SA colonised the colon the strongest. These strains may be considered for the competitive exclusion of L. monocytogenes from the GIT.

scholarly journals Induced Biliary Excretion of Listeria monocytogenes

2006 ◽  
Vol 74 (3) ◽  
pp. 1819-1827 ◽  
Author(s):  
Jonathan Hardy ◽  
Jeffrey J. Margolis ◽  
Christopher H. Contag
Keyword(s):  
Listeria Monocytogenes ◽  
Biliary Excretion ◽  
Intestinal Tract ◽  
Whole Body ◽  
Intestinal Lumen ◽  
Whole Body Imaging ◽  
Gallbladder Contraction ◽  
Life Threatening ◽  
Immunocompromised Hosts ◽  
Kinetics Of

ABSTRACT Listeria monocytogenes is a ubiquitous gram-positive bacterium that can cause systemic and often life-threatening disease in immunocompromised hosts. This organism is largely an intracellular pathogen; however, we have determined that it can also grow extracellularly in animals, in the lumen of the gallbladder. The significance of growth in the gallbladder with respect to the pathogenesis and spread of listeriosis depends on the ability of the bacterium to leave this organ and be disseminated to other tissues and into the environment. Should this process be highly inefficient, growth in the gallbladder would have no impact on pathogenesis or spread, but if it occurs efficiently, bacterial growth in this organ may contribute to listeriosis and dissemination of this organism. Here, we use whole-body imaging to determine the efficacy and kinetics of food- and hormone-induced biliary excretion of L. monocytogenes from the murine gallbladder, demonstrating that transit through the bile duct into the intestine can occur within 5 min of induction of gallbladder contraction by food or cholecystokinin and that movement of bacteria through the intestinal lumen can occur very rapidly in the absence of fecal material. These studies demonstrate that L. monocytogenes bacteria replicating in the gallbladder can be expelled from the organ efficiently and that the released bacteria move into the intestinal tract, where they pass into the environment and may possibly reinfect the animal.

Bacteriocin production and adhesion properties as mechanisms for the anti-listerial activity of Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA

2019 ◽  
Vol 10 (3) ◽  
pp. 329-349 ◽  
Author(s):  
W.F. van Zyl ◽  
S.M. Deane ◽  
L.M.T. Dicks
Keyword(s):  
Lactobacillus Plantarum ◽  
Molecular Evidence ◽  
Positive Role ◽  
Adhesion Properties ◽  
Enterococcus Mundtii ◽  
Probiotic Lactic Acid Bacteria ◽  
Mechanistic Basis ◽  
Gastro Intestinal Tract

Probiotics play an important role in maintaining a healthy and stable intestinal microbiota, primarily by preventing infection. Probiotic lactic acid bacteria (LAB) are known to be inhibitory to many bacterial enteric pathogens, including antibiotic-resistant strains. Whilst the positive role that probiotics have on human physiology, specifically in the treatment or prevention of specific infectious diseases of the gastro-intestinal tract (GIT) is known, the precise mechanistic basis of these effects remains a major research goal. In this study, molecular evidence to underpin the protective and anti-listerial effect of Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA against orally administered Listeria monocytogenes EGDe in the GIT of mice is provided. Bacteriocins plantaricin 423 and mundticin ST4SA, produced by L. plantarum 423 and E. mundtii ST4SA, respectively, inhibited the growth of L. monocytogenes in vitro and in vivo. Bacteriocin-negative mutants of L. plantarum 423 and E. mundtii ST4SA failed to exclude L. monocytogenes EGDe from the gastrointestinal tract (GIT) of mice. Furthermore, L. plantarum 423 and E. mundtii ST4SA failed to inhibit recombinant strains of L. monocytogenes EGDe in vivo that expressed the immunity proteins of the two bacteriocins. These results confirmed that bacteriocins plantaricin 423 and mundticin ST4SA acted as anti-infective mediators in vivo. Compared to wild type strains, mutants of L. plantarum 423 and E. mundtii ST4SA, in which the adhesion genes were knocked out, were less effective in the exclusion of L. monocytogenes EGDe from the GIT of mice. This work demonstrates the importance of bacteriocin and adhesion genes as probiotic anti-infective mechanisms.

scholarly journals Two Novel Type II Restriction-Modification Systems Occupying Genomically Equivalent Locations on the Chromosomes of Listeria monocytogenes Strains

2012 ◽  
Vol 78 (8) ◽  
pp. 2623-2630 ◽  
Author(s):  
Sangmi Lee ◽  
T. J. Ward ◽  
R. M. Siletzky ◽  
S. Kathariou
Keyword(s):  
Listeria Monocytogenes ◽  
Gc Content ◽  
Restriction Enzymes ◽  
Genomic Region ◽  
Type Ii ◽  
Site Specific ◽  
Content Type ◽  
Food Borne ◽  
Life Threatening ◽  
Restriction Modification

ABSTRACTListeria monocytogenesis responsible for the potentially life-threatening food-borne disease listeriosis. One epidemic-associated clonal group ofL. monocytogenes, epidemic clone I (ECI), harbors a Sau3AI-like restriction-modification (RM) system also present in the same genomic region in certain strains of other lineages. In this study, we identified and characterized two other, novel type II RM systems, LmoJ2 and LmoJ3, at this same locus. LmoJ2 and LmoJ3 appeared to recognize GCWGC (W = A or T) and GCNGC, respectively. Both RM systems consisted of genes with GC content below the genome average and were in the same genomic region in strains of different serotypes and lineages, suggesting site-specific horizontal gene transfer. Genomic DNA from the LmoJ2 and LmoJ3 strains grown at various temperatures (4 to 42°C) was resistant to digestion with restriction enzymes recognizing GCWGC or GCNGC, indicating that the methyltransferases were expressed under these conditions. Phages propagated in an LmoJ2-harboring strain exhibited moderately increased infectivity for this strain at 4 and 8°C but not at higher temperatures, while phages propagated in an LmoJ3 strain had dramatically increased infectivity for this strain at all temperatures. Among the sequencedListeriaphages, lytic phages possessed significantly fewer recognition sites for these RM systems than lysogenic phages, suggesting that in lytic phages sequence content evolved toward reduced susceptibility to such RM systems. The ability of LmoJ2 and LmoJ3 to protect against phages may affect the efficiency of phages as biocontrol agents forL. monocytogenesstrains harboring these RM systems.

Behaviour of Listeria monocytogenes isolates through gastro-intestinal tract passage simulation, before and after two sub-lethal stresses

2012 ◽  
Vol 30 (1) ◽  
pp. 24-28 ◽  
Author(s):  
J. Barbosa ◽  
S. Borges ◽  
R. Magalhães ◽  
V. Ferreira ◽  
I. Santos ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Intestinal Tract ◽  
Before And After ◽  
Gastro Intestinal Tract

A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production

Anaerobe ◽  
2012 ◽  
Vol 18 (4) ◽  
pp. 436-444 ◽  
Author(s):  
Riadh Ben Salah ◽  
Imen Trabelsi ◽  
Riadh Ben Mansour ◽  
Saloua Lassoued ◽  
Hichem Chouayekh ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Intestinal Tract ◽  
Cytokine Production ◽  
Gastro Intestinal Tract
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