Randomised double-blind placebo-controlled study of the effect of Lactobacillus paracasei NCC 2461 on skin reactivity

2014 ◽  
Vol 5 (2) ◽  
pp. 137-145 ◽  
Author(s):  
A. Gueniche ◽  
D. Philippe ◽  
P. Bastien ◽  
G. Reuteler ◽  
S. Blum ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
The Body ◽  
Lactobacillus Paracasei ◽  
Controlled Study ◽  
Skin Barrier Function ◽  
Skin Sensitivity ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Function Recovery

In recent decades, the prevalence of subjects with reactive skin has considerably increased in industrialised countries. 50% of women and 30% of men report cutaneous discomfort classified under reactive/sensitive skin. Several topical approaches have been proposed, in particular through improvement of galenic forms or protection of epidermal surface. We propose to act differently, deeply from inside the body via an innovative nutritional approach. To this purpose, Lactobacillus paracasei NCC 2461 (ST11) was selected because of its specific beneficial skin properties discovered in in vitro studies, i.e. diminution of neurogenic inflammation and promotion of the recovery of skin barrier function. We designed a randomised double-blind placebo-controlled clinical study with a two-month supplementation in two female treatment groups (n=32 per group). A capsaicin test was performed to monitor the time course of skin sensitivity. Moreover, transepidermal water loss was assessed to analyse the rate of skin barrier function recovery; dryness of the leg and roughness of the cheeks was investigated by a dermatologist as well as by self-assessment. The results of the present clinical trial show that oral supplementation with the probiotic decreases skin sensitivity and increases the rate of barrier function recovery. Thus, the data provide evidence that daily intake of ST11 could improve reactive skin condition.

scholarly journals Hydrogel-Forming Microneedles Increase in Volume During Swelling in Skin, but Skin Barrier Function Recovery is Unaffected

2014 ◽  
Vol 103 (5) ◽  
pp. 1478-1486 ◽  
Author(s):  
Ryan F. Donnelly ◽  
Karen Mooney ◽  
Maelíosa T.C. Mccrudden ◽  
Eva M. Vicente-Pérez ◽  
Luc Belaid ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Function Recovery

Skin barrier function recovery after diamond microdermabrasion

2009 ◽  
Vol 36 (10) ◽  
pp. 529-533 ◽  
Author(s):  
Hei Sung KIM ◽  
Sook Hee LIM ◽  
Ji Youn SONG ◽  
Mi-Yeon KIM ◽  
Ji Ho LEE ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Function Recovery

scholarly journals Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study

2011 ◽  
Vol 107 (6) ◽  
pp. 876-884 ◽  
Author(s):  
Giuliano Rizzardini ◽  
Dorte Eskesen ◽  
Philip C. Calder ◽  
Amedeo Capetti ◽  
Lillian Jespersen ◽  
...  
Keyword(s):  
Influenza Vaccination ◽  
Effective Means ◽  
Innate Immune ◽  
Lactobacillus Paracasei ◽  
Secretory Iga ◽  
Controlled Study ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Bifidobacterium Animalis ◽  
Immune Parameters

The present study investigated the ability of Bifidobacterium animalis ssp. lactis (BB-12®) and Lactobacillus paracasei ssp. paracasei (L. casei 431®) to modulate the immune system using a vaccination model in healthy subjects. A randomised, double-blind, placebo-controlled, parallel-group study was conducted in 211 subjects (56 % females, mean age 33·2 (sd 13·1) years). Subjects consumed a minimum of 109 colony-forming units of BB-12® (capsule) or L. casei 431® (dairy drink) or a matching placebo once daily for 6 weeks. After 2 weeks, a seasonal influenza vaccination was given. Plasma and saliva samples were collected at baseline and after 6 weeks for the analysis of antibodies, cytokines and innate immune parameters. Changes from baseline in vaccine-specific plasma IgG, IgG1 and IgG3 were significantly greater in both probiotic groups v. the corresponding placebo group (L. casei 431®, P = 0·01 for IgG; P < 0·001 for remaining comparisons). The number of subjects obtaining a substantial increase in specific IgG (defined as ≥ 2-fold above baseline) was significantly greater in both probiotic groups v. placebo (BB-12®, P < 0·001 for IgG, IgG1 and IgG3; L. casei 431®, P < 0·001 for IgG1 and IgG3). Significantly greater mean fold increases for vaccine-specific secretory IgA in saliva were observed in both probiotic groups v. placebo (BB-12®, P = 0·017; L. casei 431®, P = 0·035). Similar results were observed for total antibody concentrations. No differences were found for plasma cytokines or innate immune parameters. Data herein show that supplementation with BB-12® or L. casei 431® may be an effective means to improve immune function by augmenting systemic and mucosal immune responses to challenge.

Supplementation of Flaxseed Oil Diminishes Skin Sensitivity and Improves Skin Barrier Function and Condition

2011 ◽  
Vol 24 (2) ◽  
pp. 67-74 ◽  
Author(s):  
K. Neukam ◽  
S. De Spirt ◽  
W. Stahl ◽  
M. Bejot ◽  
J.-M. Maurette ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Flaxseed Oil ◽  
Skin Barrier Function ◽  
Skin Sensitivity

scholarly journals Clinical Evaluation of Topical Lotion Containing 2-Aza-8-Oxohypoxanthine on Skin Barrier Function against Water Loss

Cosmetics ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 83
Author(s):  
Hisae Aoshima ◽  
Rinta Ibuki ◽  
Masayuki Ito ◽  
Hirokazu Kawagishi
Keyword(s):  
Moisture Content ◽  
Stratum Corneum ◽  
Water Loss ◽  
Barrier Function ◽  
Statistical Difference ◽  
Skin Barrier ◽  
Comparative Trial ◽  
Skin Barrier Function ◽  
Double Blind ◽  
Split Face

A clinical study was conducted to evaluate the efficacy of 2-aza-8-oxohypoxanthine (AOH) on human skin in a double-blind, placebo-controlled, and split-face comparative trial. To this end, a topical lotion containing 0.1% of AOH and its placebo formulation were applied in 20 Japanese subjects (age: 41–58 years, average: 48.4 ± 4.7 years) to their faces daily for eight weeks. The moisture content in the stratum corneum and the trans-epidermal water loss (TEWL) were measured at the beginning of the study and after eight weeks. The results obtained upon comparing the data of the two measuring points showed that the 0.1% AOH lotion caused a statistically significant larger decrease in TEWL after eight weeks. Moreover, the 0.1% AOH lotion produced a larger increase in the moisture content in the stratum corneum after eight weeks, although there was no statistical difference between the two formulations. These results clearly indicate that AOH is effective as a cosmetic agent with a skin barrier function against water loss.

scholarly journals Oryza Ceramide®, a rice-derived extract consisting of glucosylceramides and β-sitosterol glucoside, improves facial skin dehydration in Japanese subjects

2021 ◽  
Vol 11 (8) ◽  
pp. 385
Author(s):  
Tsuyoshi Takara ◽  
Kazuo Yamamoto ◽  
Naoko Suzuki ◽  
Shinichiro Yamashita ◽  
Shin-ichiro Iio ◽  
...  
Keyword(s):  
Water Loss ◽  
Random Number Generator ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Controlled Study ◽  
Skin Barrier Function ◽  
Facial Skin ◽  
Double Blind ◽  
Hydrophobic Fraction

Background and objective: The ingestion of plant-derived glucosylceramides (GlcCer) has been reported to contribute to skin barrier function and hydration of the epidermis. b-sitosterol glucoside (BSG) colocalized with GlcCer in the rice hydrophobic fraction has been shown to increase ceramides in the stratum corneum in vitro. Although clinical studies demonstrated that GlcCer reduced transepidermal water loss (TEWL), the contribution of BSG to epidermal dehydration when applied with GlcCer remains unknown. Therefore, we herein conducted a clinical trial on the effects of a rice-derived mixed fraction of GlcCer and BSG (Oryza Ceramide®) on TEWL and other skin parameters. Methods: A randomized, double-blind, placebo-controlled study design was used. Oryza Ceramide® (type PCD, 40 mg daily) containing 1.2 mg of GlcCer and 40 mg of BSG was used as the active sample. We enrolled 44 healthy Japanese women with epidermal dehydration. All subjects were randomly allocated to an active group (n=22) or placebo group (n=22) using a computerized random number generator. Capsules containing the active sample or placebo were administered for 12 weeks between August and December 2020. Cheek TEWL after 12 weeks was assessed as the primary outcome, and TEWL on a different part of the skin and various skin parameters, including epidermal moisture, pigmentation, pores, and elasticity, were measured before and after 4, 8, and 12 weeks of the intervention. Blood, urine, and body parameters were also examined to evaluate safety.Results: Forty-four subjects completed the trial, and the per protocol set comprised 22 each in the active and placebo groups. Cheek TEWL significantly reduced after the Oryza Ceramide® intervention for 4 and 12 weeks. Among the secondary outcomes examined, lip moisture (12 weeks) and visible pore number (4 weeks) were improved by Oryza Ceramide®. Laboratory tests revealed no abnormalities to suggest any adverse effects of Oryza Ceramide®.Conclusions: Oryza Ceramide® (40 mg/day) consisting of GlcCer and BSG improved facial TEWL, lip moisture, and visible pores, and these effects may be attributed to increases in epidermal ceramides. The combination of rice GlcCer and BSG appears to be beneficial for improving facial skin conditions.Trial Registration: UMIN-CTR: UMIN000041295Foundation: The study was funded by Oryza Oil & Fat Chemical Co., Ltd. and Aichi Prefectural Subsidies for Research and Development of Creative Products in 2020.Keywords: rice; glucosylceramide; β-sitosterol glucoside; trans epidermal water loss; pore 

scholarly journals Retaining Skin Barrier Function Properties of the Stratum Corneum with Components of the Natural Moisturizing Factor—A Randomized, Placebo-Controlled Double-Blind In Vivo Study

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1649
Author(s):  
Johannes Schleusener ◽  
Andrew Salazar ◽  
Jörg von Hagen ◽  
Jürgen Lademann ◽  
Maxim E. Darvin
Keyword(s):  
Stratum Corneum ◽  
Barrier Function ◽  
Bound Water ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Double Blind Study ◽  
Weakly Bound ◽  
Double Blind

The influence of a topically applied formulation containing components of natural moisturizing factor (NMF) on barrier-related parameters of the stratum corneum (SC) was investigated in vivo using confocal Raman microspectroscopy in a randomized, placebo-controlled double-blind study on 12 volunteers for 14 days. This method allowed for the elucidation of subtle differences between the verum and the placebo even though the components of the verum naturally occur in the SC. This differentiation is not possible non-invasively by conventional methods. In this study, we found that the applied verum and placebo formulations disrupted the equilibrium of water, NMF and lipids in the SC. The adverse effects of the formulation could be mitigated by incorporating it into a simplified supplementation of NMF molecules. As a long-term effect, the amount of strongly bound water increases at 30–40% SC depth (p < 0.05) and the amount of weakly bound water decreases at 30–40% SC depth (p < 0.05) for the verum. This supplement was also unexpectedly able to prevent intercellular lipids (ICL) disorganization in selected depths. In the long term, the verum treatment limited the lateral disorganization of the ICL to the upper 20% SC depth. Further research is required to elucidate the interplay of these factors in the SC, to better understand their contribution to the equilibrium and barrier function of the skin. This understanding of the interaction of these naturally occurring components could help in the future to develop and optimize topical treatments for diseases like psoriasis, atopic dermatitis, ichthyosis where the skin barrier is disrupted.

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