scholarly journals The production and adsorption of inflammatory cytokines by closed loop circulation of whole blood with hemofilters

1998 ◽  
Vol 5 (1) ◽  
pp. 33-38
Author(s):  
Toshio Naka ◽  
Masahiro Shinozaki ◽  
Toshihiko Morinaga ◽  
Yoshiaki Tomobuchi ◽  
Koichi Kuribayashi ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Closed Loop ◽  
Loop Circulation

The production of anti-inflammatory cytokines in whole blood by physico-chemical induction

2003 ◽  
Vol 52 (10) ◽  
pp. 404-407 ◽  
Author(s):  
H. Meijer ◽  
J. Reinecke ◽  
C. Becker ◽  
G. Tholen ◽  
P. Wehling
Keyword(s):  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Chemical Induction ◽  
Physico Chemical ◽  
Anti Inflammatory

scholarly journals Physiologically relevant aspirin concentrations trigger immunostimulatory cytokine production by human leukocytes

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0254606
Author(s):  
Regine Brox ◽  
Holger Hackstein
Keyword(s):  
Acetylsalicylic Acid ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Plasma Concentrations ◽  
Immunomodulatory Activity ◽  
Prostaglandin E ◽  
Dependent Manner ◽  
Cox 2 ◽  
Anti Inflammatory

Acetylsalicylic acid is a globally used non-steroidal anti-inflammatory drug (NSAID) with diverse pharmacological properties, although its mechanism of immune regulation during inflammation (especially at in vivo relevant doses) remains largely speculative. Given the increase in clinical perspective of Acetylsalicylic acid in various diseases and cancer prevention, this study aimed to investigate the immunomodulatory role of physiological Acetylsalicylic acid concentrations (0.005, 0.02 and 0.2 mg/ml) in a human whole blood of infection-induced inflammation. We describe a simple, highly reliable whole blood assay using an array of toll-like receptor (TLR) ligands 1–9 in order to systematically explore the immunomodulatory activity of Acetylsalicylic acid plasma concentrations in physiologically relevant conditions. Release of inflammatory cytokines and production of prostaglandin E2 (PGE2) were determined directly in plasma supernatant. Experiments demonstrate for the first time that plasma concentrations of Acetylsalicylic acid significantly increased TLR ligand-triggered IL-1β, IL-10, and IL-6 production in a dose-dependent manner. In contrast, indomethacin did not exhibit this capacity, whereas cyclooxygenase (COX)-2 selective NSAID, celecoxib, induced a similar pattern like Acetylsalicylic acid, suggesting a possible relevance of COX-2. Accordingly, we found that exogenous addition of COX downstream product, PGE2, attenuates the TLR ligand-mediated cytokine secretion by augmenting production of anti-inflammatory cytokines and inhibiting release of pro-inflammatory cytokines. Low PGE2 levels were at least involved in the enhanced IL-1β production by Acetylsalicylic acid.

scholarly journals Fc-gamma receptor expression and cytokine response to intravenous human immunoglobulin in mothers and neonates

2021 ◽  
Author(s):  
Stephania Vazquez-Rodriguez ◽  
Lourdes A. Arriaga-Pizano ◽  
Ismael Mancilla-Herrera ◽  
Jessica Prieto-Chávez ◽  
Roberto Arizmendi-Villanueva ◽  
...  
Keyword(s):  
Adjuvant Therapy ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Blood Cultures ◽  
Receptor Expression ◽  
Human Immunoglobulin ◽  
List Type ◽  
Fc Gamma Receptor ◽  
Gamma Receptor ◽  
Pro Inflammatory Cytokines

AbstractObjectiveThe use of intravenous human immunoglobulin (IVIg) as adjuvant therapy for sepsis has been shown efficacious in adults, but its use in pregnant women and newborns is controversial. Fc gamma receptors (FcγRs) support the ability of IVIg to stimulate the synthesis of inflammatory mediators and promote phagocytosis by leukocytes, however, the FcγRs expression is differential between adults and neonates. We aimed to explore the effect of IVIg in monocytes and neutrophils from mother and neonates in whole blood cultures.Study designWhole blood from adults, maternal, and neonates were incubated with LPS and/or IVIg. After 0, 24, and 48 hours, we measured the expression of FcγRs (CD16, CD32, and CD64) and bacterial phagocytosis by monocytes and neutrophils. Also, the concentration of pro-inflammatory cytokines/chemokines was determined.ResultsFcγRs expression is quite similar among groups, and the LPS or IVIg challenge did not change the FcγRs expression on monocytes and neutrophils. Also, the LPS or IVIg challenge did not modify phagocytosis capacity in any group. However, IVIg induces a higher IL-8 response in neonates than in adults.ConclusionOur results suggest that the IL-8 response to IVIg in whole blood from neonates is not dependent on differential FcγR expression.Key messagesIVIg challenge in neonates or adults does not induce FcγR change expression on monocytes or neutrophilsIVIg induces higher IL-8 response in neonates than in adults

Clot Accumulation Characteristics of Plasminogen-bearing Liposomes in a Flow-system

1998 ◽  
Vol 79 (01) ◽  
pp. 144-149 ◽  
Author(s):  
H. Feitsma ◽  
C. Kluft ◽  
J. L. M. Heeremans ◽  
R. Prevost ◽  
D. J. A. Crommelin
Keyword(s):  
Closed System ◽  
Whole Blood ◽  
Surface Density ◽  
Closed Loop ◽  
Flow System ◽  
Blood Clot ◽  
Fibrin Clot ◽  
Partial Degradation ◽  
Accumulation Characteristics

SummaryIn this study, the clot accumulation properties of liposome-coupled plasminogen were compared to those of free (non-liposomal) plasminogen in an in vitro, closed-loop, flow-system. After introduction of a clot into the closed system, double-radiolabelled plasminogen-liposomes were administered and the accumulation of radiolabel on the entire clot was measured.Liposomal plasminogen showed improved accumulation over free plasminogen, on both a fibrin clot and a whole blood clot. Moreover, once liposomal plasminogen was fibrin associated, it could not be washed away with buffer, in contrast to free plasminogen. Liposomal plasminogen was able to compete successfully with an excess of free plasminogen. The plateau levels for the accumulated amount of plasminogen depended on the incubated amount of plasminogen and were influenced by partial degradation of the clot. Furthermore, it was shown that a threshold liposomal plasminogen surface-density was needed for optimum clot accumulation.

Li experiments on T-11M and T-10 in support of a steady-state tokamak concept with Li closed loop circulation

2011 ◽  
Vol 51 (7) ◽  
pp. 073044 ◽  
Author(s):  
S.V. Mirnov ◽  
E.A. Azizov ◽  
A.G. Alekseev ◽  
V.B. Lazarev ◽  
R.R. Khayrutdinov ◽  
...  
Keyword(s):  
Steady State ◽  
Closed Loop ◽  
Loop Circulation

scholarly journals Effect of flavonoid-rich meals and low-flavonoid meals based on the dietary reference intakes for Japanese, using basic foodstuffs on the gene expression of inflammatory cytokines in the whole blood cells from adult men of normal or light overweight

2021 ◽  
Vol 11 (2) ◽  
pp. 56
Author(s):  
Ryo Mannen ◽  
Michiko T. Yasuda ◽  
Ayami Sano ◽  
Toshinao Goda ◽  
Kayoko Shimoi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Blood Cells ◽  
Inflammatory Cytokine ◽  
Disease Risk ◽  
Human Study ◽  
Cytokine Gene Expression ◽  
Adult Men ◽  
Whole Blood Cells

Introduction: Flavonoids have a variety of functions, such as antioxidant activity, and are expected to have a disease prevention effect. In order to verify the disease risk reduction effect of flavonoids, we carried out a crossover trial in seven adult men of normal or light overweight who ingested flavonoid-rich meals, with a diverse combination of basic foodstuffs, and low-flavonoid meals and compared blood disease-related inflammatory markers.Methods: On the first two days of the study, seven male volunteers were provided with low-flavonoid meals (flavonoid content below the detection limit of HPLC: less than 0.24 mg/meal) three times a day as a washout. For the next seven days, they were fed flavonoid-rich meals (46.9 ± 8.1 mg/meal) or low-flavonoid meals. Blood samples were collected from all the volunteers before breakfast on the third day, after the washout and before breakfast on the tenth day. The test was consisted of one cycle from the first day to the tenth day, and the participants carried out two cycles. Flavonoid concentrations in plasma and gene expression of inflammatory cytokine (interleukin 1 beta, interleukin 6, interleukin 18, and tumor necrosis factor-α) in whole blood cells were compared before and after the intervention. Gene expression in whole blood cells was measured using real time RT-PCR.Results: We found a significant increase in plasma flavonoid concentration (quercetin, kaempferol, daidzein, and genistein) upon intervention with flavonoid-rich meals (p < 0.05). In addition, the inflammatory cytokine gene expression was reduced in the subjects with a body mass index of more than, but not less than, 25 kg/m2 compared with that observed after the intake of low-flavonoid meals.Conclusion: These results suggest that flavonoid-rich meals have an anti-inflammatory effect in obese persons who are likely to have chronic inflammation.Keywords: Flavonoids, inflammatory cytokines, flavonoid-rich meal, human study

scholarly journals Effects of JAK1-Preferential Inhibitor Filgotinib on Circulating Biomarkers and Whole Blood Genes/Pathways of Patients With Moderately to Severely Active Crohn’s Disease

2021 ◽  
Author(s):  
Xavier Roblin ◽  
Adrian Serone ◽  
Oh Kyu Yoon ◽  
Luting Zhuo, ◽  
Ethan Grant ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Disease Activity ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Janus Kinase ◽  
Oncostatin M ◽  
Circulating Biomarkers ◽  
Amyloid A ◽  
Pro Inflammatory Cytokines

Abstract Background Pro-inflammatory cytokines are dysregulated in Crohn’s disease (CD) and could serve as surrogate markers to improve diagnostic and therapeutic approaches, potentially addressing an unmet need. We profiled circulating biomarkers and whole blood transcriptional pathway activity to identify those associated with CD using data from the phase 2 FITZROY study with filgotinib, an oral preferential janus kinase-1 inhibitor. Methods Patients with serum and whole blood samples taken from the induction period were included. Serum cytokines were measured (ELISA), whole blood RNA sequenced, and stool samples taken to measure fecal calprotectin (FC). Spearman’s Rank correlations were assessed between biomarkers and baseline disease activity; post-treatment endoscopic improvement was measured by the Simplified Endoscopy Score for CD (SES-CD), FC and the Crohn’s Disease Activity Index. Effect of filgotinib on circulating biomarkers was also evaluated. Results Serum biomarkers (n = 168) and whole blood RNA sequencing (n = 104) were assessed. Moderate correlation between serum analytes with SES-CD and FC was noted; most highly correlated were acute phase proteins CRP (rho = 0.35 [SES-CD] and 0.47 [FC]), serum amyloid A (rho = 0.40 and 0.39, respectively) and pro-inflammatory cytokines interleukin (IL)-6 (rho = 0.31 and 0.30, respectively), IL-22 (rho = 0.36 and 0.35, respectively), and oncostatin M (rho = 0.35 and 0.33, respectively). Filgotinib treatment was associated with reduction of many candidate biomarkers, particularly in patients with treatment response. Early changes in IL-6 and IL-10 may be prognostic for endoscopic response. Conclusions Several circulating factors with potential as CD activity biomarkers were identified. Larger studies are necessary to investigate the best utility of these markers for CD.

Sign in / Sign up

Export Citation Format

Share Document