scholarly journals Immunomodulatory Effects of Helicobacter pylori on Pro and Anti-inflammatory Cytokines Production in Peripheral Whole Blood Cells Culture

2016 ◽  
Vol 13 (4) ◽  
pp. 2221-2230
Author(s):  
Nazila Bahmaie ◽  
Soghrat Faghihzadeh ◽  
Abdolreza Esmaeilzadeh ◽  
Bahram Amini
Keyword(s):  
Helicobacter Pylori ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Blood Cells ◽  
Immunomodulatory Effects ◽  
Anti Inflammatory ◽  
Whole Blood Cells

scholarly journals INFLUENCE OF THERAPY UPON LPS-INDUCED CYTOKINE SECRETION BY THE BLOOD-DERIVED INNATE IMMUNITY CELLS OF THE BRONCHIAL ASTHMA PATIENTS

2019 ◽  
Vol 21 (4) ◽  
pp. 789-796
Author(s):  
D. A. Serov ◽  
D. S. Kabanov ◽  
N. I. Kosyakova ◽  
I. R. Prokhorenko
Keyword(s):  
Healthy Volunteers ◽  
Immune Cells ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Innate Immune ◽  
Innate Immune Cells ◽  
Blood Samples ◽  
Anti Inflammatory ◽  
Whole Blood Cells

Bronchial asthma (BA) is the most widespread chronic inflammatory disease. Since BA is associated with a systemic inflammation state, a comprehensive study of its effect in this disease, and influence of pathogenetic therapy should be performed, by studying the whole blood cytokine status of the patients suffering with BA. The cells from respiratory tract in acute-phase BA patients may produce pro-, as well as anti-inflammatory mediators. The anti-inflammatory mediators are able to suppress activity of immune cells in peripheral blood. Thus, the aim of present study was to evaluate eventual inflammation-associated and functional activity of immune cells from the patients’ peripheral blood in BA and following appropriate therapy. Bacterial lipopolysaccharide (LPS) a classical pro-inflammatory agent. We have studied an LPSinduced cytokine-induced ex vivo secretion model by peripheral blood immune cells, as a relevant test for their functional activity. The LPS-induced responses of whole blood cells from patients with proven BA diagnosis have been studied at pre-treatment time points, and following two weeks of basic anti-inflammatory therapy. According to clinical indications, the antagonists of CysLTR1, or combinations of glucocorticosteroids and β-adrenoreceptor agonists were administered by inhalation to BA patients. LPS-induced production of TNFα, IL-6, IL-8 (at 6 h) and IFNγ, IL-17A or IL-1β (at 24 h) by whole blood cells from BA patients or healthy volunteers has been assessed by ELISA technique. The cytokine production from non-stimulated whole blood cells from BA patients and healthy volunteers were used as the baseline control. IL-4 concentrations in plasma of BA patients and healthy volunteers were also measured. We have shown a decrease of IL-6 production in control blood samples from BA patients after two weeks of therapy. This may indicate the attenuation of the observed inflammatory process. The therapy applied did not influence the background levels and LPS-induced secretion of IL-1β, IL-1ra, IFNγ, and IL-8 in whole blood samples from BA patients. IL-4 plasma levels in BA patients were not changed after two weeks of therapy. It has been shown that whole blood from BA patients produced less TNFα and IL-8, both in control samples, and during their response to LPS, than the values obtained in healthy volunteers. These findings are in agreement with a notion that BA causes partial depression of innate immune cells activity. The increased LPS-induced TNFα secretion by the whole blood cells from BA patients has been observed following two weeks of basic anti-inflammatory therapy. We suggest that the increased LPS-induced TNFα secretion could be explained by partial restoration of peripheral blood immune cell activity associated with anti-inflammatory BA therapy. To elucidate the mechanism of increased LPS-induced TNFα secretion, we have estimated whole blood concentration of soluble CD14 (sCD14) in BA patients. No significant differences between sCD14 concentrations have been found. Obtained result presume existence of sCD14-independent mechanism of TNFα regulation by whole blood cells in response on LPS which may occur during anti-inflammatory therapy of BA. We suppose that basic anti-inflammatory therapy of BA does not simply reduce IL-6 concentration in peripheral blood, but may also partially restore the activity of innate immune cells in BA patients.

scholarly journals Effect of flavonoid-rich meals and low-flavonoid meals based on the dietary reference intakes for Japanese, using basic foodstuffs on the gene expression of inflammatory cytokines in the whole blood cells from adult men of normal or light overweight

2021 ◽  
Vol 11 (2) ◽  
pp. 56
Author(s):  
Ryo Mannen ◽  
Michiko T. Yasuda ◽  
Ayami Sano ◽  
Toshinao Goda ◽  
Kayoko Shimoi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Blood Cells ◽  
Inflammatory Cytokine ◽  
Disease Risk ◽  
Human Study ◽  
Cytokine Gene Expression ◽  
Adult Men ◽  
Whole Blood Cells

Introduction: Flavonoids have a variety of functions, such as antioxidant activity, and are expected to have a disease prevention effect. In order to verify the disease risk reduction effect of flavonoids, we carried out a crossover trial in seven adult men of normal or light overweight who ingested flavonoid-rich meals, with a diverse combination of basic foodstuffs, and low-flavonoid meals and compared blood disease-related inflammatory markers.Methods: On the first two days of the study, seven male volunteers were provided with low-flavonoid meals (flavonoid content below the detection limit of HPLC: less than 0.24 mg/meal) three times a day as a washout. For the next seven days, they were fed flavonoid-rich meals (46.9 ± 8.1 mg/meal) or low-flavonoid meals. Blood samples were collected from all the volunteers before breakfast on the third day, after the washout and before breakfast on the tenth day. The test was consisted of one cycle from the first day to the tenth day, and the participants carried out two cycles. Flavonoid concentrations in plasma and gene expression of inflammatory cytokine (interleukin 1 beta, interleukin 6, interleukin 18, and tumor necrosis factor-α) in whole blood cells were compared before and after the intervention. Gene expression in whole blood cells was measured using real time RT-PCR.Results: We found a significant increase in plasma flavonoid concentration (quercetin, kaempferol, daidzein, and genistein) upon intervention with flavonoid-rich meals (p < 0.05). In addition, the inflammatory cytokine gene expression was reduced in the subjects with a body mass index of more than, but not less than, 25 kg/m2 compared with that observed after the intake of low-flavonoid meals.Conclusion: These results suggest that flavonoid-rich meals have an anti-inflammatory effect in obese persons who are likely to have chronic inflammation.Keywords: Flavonoids, inflammatory cytokines, flavonoid-rich meal, human study

scholarly journals СONCENTRATION OF ANTI-INFLAMATORY CYTOKINES IN CELL CULTURE SUPERNATANTS IN CHILDREN WITH JUVENILE IDIOPATHIC ARTHRITIS

2019 ◽  
Vol 21 (4) ◽  
pp. 725-736
Author(s):  
I. M. Krivolapova ◽  
I. A. Pashnina ◽  
V. A. Chereshnev
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Cultures ◽  
Juvenile Arthritis ◽  
Peripheral Blood Cells ◽  
Healthy Children ◽  
Anti Inflammatory

Juvenile idiopathic arthritis is a chronic inflammatory disease of the joints in children, mainly of autoimmune or auto-inflammatory nature. It is a heterogeneous group, which includes different subtypes of the disease. Different mechanisms may play role in the pathogenesis of distinct subtypes of juvenile arthritis. However, a long-term imbalance of pro- and anti-inflammatory cytokines is important for all subtypes of disease. The aim of the present study was to determine spontaneous and stimulated anti-inflammatory cytokines production by peripheral blood cells from the children with juvenile idiopathic arthritis. Patients of 2 to 17 years old with different subtypes of juvenile idiopathic arthritis (n = 99) and healthy children without signs of autoimmune diseases (control, n = 31) were examined. Spontaneous and phytohemagglutinin-stimulated concentrations of IL-1ra, IL-4, IL-10, TGF-β in supernatants of whole-blood cultures were determined by ELISA. Differences in the spontaneous and mitogen-stimulated secretion of the cytokines in patients with different subtypes of juvenile arthritis have not been revealed. The spontaneous IL-1ra, IL-4 and IL-10 production by blood cells in the common group of patients with juvenile idiopathic arthritis was similar to the controls. The median value of spontaneous TGF-β concentration in the patients was below the detection level, whereas blood cells of healthy children had a higher potential of spontaneous TGF-β production. IL-4 and IL-10 production after incubation of peripheral blood cells with phytohemagglutinin in patients and in the control group did not differ from the controls, while IL-1ra and TGF-β synthesis was significantly lower than in healthy children.The spontaneous and/or stimulated production of IL-1ra, TGF-β by blood cells in children with juvenile idiopathic arthritis reflects the pathogenic significance of these cytokines in disease. Stimulation of cells can reveal a latent deficiency in the synthesis of cytokines, which is not evident when determining its concentration in serum or supernatants of spontaneous whole-blood cultures.

Immunomodulatory effects of Sanglifehrin A in the innate and acquired immune response of neonatal whole blood cells

Immunobiology ◽  
2009 ◽  
Vol 214 (3) ◽  
pp. 235-243
Author(s):  
Christoph Härtel ◽  
Jan Rupp ◽  
Peter Iblher ◽  
Alexander Puzik ◽  
Ines Osthues ◽  
...  
Keyword(s):  
Immune Response ◽  
Whole Blood ◽  
Blood Cells ◽  
Immunomodulatory Effects ◽  
Sanglifehrin A ◽  
Whole Blood Cells

scholarly journals Identification of Cytokines in Whole Blood for Differential Diagnosis of Tuberculosis versus Pneumonia

2010 ◽  
Vol 17 (5) ◽  
pp. 771-777 ◽  
Author(s):  
Wen-Lin Su ◽  
Wann-Cherng Perng ◽  
Ching-Hui Huang ◽  
Cheng-Yu Yang ◽  
Chin-Pyng Wu ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Cutoff Point ◽  
Interleukin 12 ◽  
Operating Characteristics ◽  
Percent Change ◽  
Ifn Γ ◽  
Sensitivity Specificity ◽  
Whole Blood Cells ◽  
Stimulation Of

ABSTRACT Differentiating tuberculosis (TB) from pneumonia remains a challenge. We evaluated the cytokine profiles of whole blood cells from patients with TB (n = 38) or pneumonia (n = 30) and from healthy individuals (n = 30) before and after stimulating cells with ESAT-6 or lipopolysaccharide (LPS). When the percent change in the levels of gamma interferon (IFN-γ) after stimulation with ESAT-6 was used in receiver operating characteristics (ROC) analysis (a graphic method to determine the diagnostic accuracy of a test) to identify a patient with TB, the area under the curve (AUC) was 90.4%, and a cutoff point of a 3.59% change produced a corresponding sensitivity, specificity, and accuracy of over 80%. When the change in IFN-γ after stimulation of blood cells with LPS was used to identify a patient with pneumonia, the AUC reached 89.1%, and a cutoff point of 3.59% produced a sensitivity, specificity, and accuracy of approximately 80% each. When the change in interleukin-12 (IL-12) after stimulation of blood cells with LPS was selected to define a patient with pneumonia, the AUC was 85.2%, and a cutoff point of 2.08% gave a sensitivity, specificity, and accuracy of 80.0%, 78.9%, and 79.4%, respectively. We conclude that the percent change in IFN-γ after stimulation of whole blood cells with ESAT-6 may differentiate patients with TB from patients with pneumonia. The percent change in IFN-γ and IL-12 after LPS stimulation of whole blood cells could differentiate patients with pneumonia from patients with TB.

scholarly journals Synthesis of the 3,5-diphenyl-1H-pyrazole and cytogenetic and oxidative alterations after exposure of cultured human whole blood cells

2017 ◽  
Vol 3 (1) ◽  
pp. 1344115 ◽  
Author(s):  
Esvet Akbas ◽  
Fatih Caglar Celikezen ◽  
Hasan Turkez ◽  
Ozlem Ozdemir ◽  
Adem Ruzgar ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Human Whole Blood ◽  
Whole Blood Cells

The production of anti-inflammatory cytokines in whole blood by physico-chemical induction

2003 ◽  
Vol 52 (10) ◽  
pp. 404-407 ◽  
Author(s):  
H. Meijer ◽  
J. Reinecke ◽  
C. Becker ◽  
G. Tholen ◽  
P. Wehling
Keyword(s):  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Chemical Induction ◽  
Physico Chemical ◽  
Anti Inflammatory

scholarly journals Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Lada Živković ◽  
Vladan Bajić ◽  
Dijana Topalović ◽  
Marija Bruić ◽  
Biljana Spremo-Potparević
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Natural Products ◽  
Whole Blood ◽  
Blood Cells ◽  
Statistical Significance ◽  
Individual Treatment ◽  
Alternative Source ◽  
Whole Blood Cells ◽  
Antigenotoxic Effects

The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.

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