scholarly journals Flow cytometric estimation of the nuclear genome size of 22Echinops (Asteraceae) taxa from Turkey

2015 ◽  
Vol 39 ◽  
pp. 580-587 ◽  
Author(s):  
Handan ŞAPCI ◽  
Monika REWERS ◽  
Cem VURAL ◽  
Elwira SLIWINSKA
Keyword(s):  
Genome Size ◽  
Nuclear Genome ◽  
Flow Cytometric

Nuclear genome size in Selaginella

Genome ◽  
2007 ◽  
Vol 50 (4) ◽  
pp. 351-356 ◽  
Author(s):  
Damon P. Little ◽  
Robbin C. Moran ◽  
Eric D. Brenner ◽  
Dennis Wm. Stevenson
Keyword(s):  
Chromosome Number ◽  
Genome Size ◽  
Nuclear Genome ◽  
Rank Correlation ◽  
Cultivated Plants ◽  
Strongly Correlated ◽  
Flow Cytometric Data ◽  
Spearman’S Rank Correlation ◽  
Time Estimates ◽  
Flow Cytometric

Estimates of nuclear genome size for 9 Selaginella species were obtained using flow cytometry, and measurements for 7 of these species are reported for the first time. Estimates range from 0.086 to 0.112 pg per holoploid genome (84–110 Mb). The data presented here agree with the previously published flow cytometric results for S. moellendorffii . Within the 9 species sampled here, chromosome number varies from 2n = 16 to 2n = 27. Nuclear genome size appears to be strongly correlated with chromosome number (Spearman’s rank correlation; p = 0.00003725). Cultivated S. moellendorffii lacks sexual reproduction—manifest by the production of abortive megasporangia. Flow cytometric data generated from a herbarium specimen of a fertile wild-collected S. moellendorffii are virtually indistinguishable from the data generated from fresh material (0.088 vs. 0.089 pg/1C). Therefore, the limited fertility observed in cultivated plants is probably not the result of abnormal chromosome number (e.g., induced by interspecific hybridization).

scholarly journals Challenges of flow-cytometric estimation of nuclear genome size in orchids, a plant group with both whole-genome and progressively partial endoreplication

2015 ◽  
Vol 87 (10) ◽  
pp. 958-966 ◽  
Author(s):  
Pavel Trávníček ◽  
Jan Ponert ◽  
Tomáš Urfus ◽  
Jana Jersáková ◽  
Jan Vrána ◽  
...  
Keyword(s):  
Genome Size ◽  
Nuclear Genome ◽  
Whole Genome ◽  
Flow Cytometric ◽  
Plant Group

scholarly journals Origin of the Eukaryotic Cell

2017 ◽  
Vol 01 (02) ◽  
pp. 108-120 ◽  
Author(s):  
Nick Lane
Keyword(s):  
Protein Synthesis ◽  
Host Cell ◽  
Genome Size ◽  
Energy Savings ◽  
Nuclear Genome ◽  
Eukaryotic Cell ◽  
Eukaryotic Cells ◽  
Bacterial Genomes ◽  
Complex Cells ◽  
Life On Earth

All complex life on Earth is composed of ‘eukaryotic’ cells. Eukaryotes arose just once in 4 billion years, via an endosymbiosis — bacteria entered a simple host cell, evolving into mitochondria, the ‘powerhouses’ of complex cells. Mitochondria lost most of their genes, retaining only those needed for respiration, giving eukaryotes ‘multi-bacterial’ power without the costs of maintaining thousands of complete bacterial genomes. These energy savings supported a substantial expansion in nuclear genome size, and far more protein synthesis from each gene.

scholarly journals Genome Size of Twenty Wild Species of Oryza Determined by Flow Cytometric and Chromosome Analyses

2007 ◽  
Vol 57 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Toshie Miyabayashi ◽  
Ken-Ichi Nonomura ◽  
Hiroko Morishima ◽  
Nori Kurata
Keyword(s):  
Genome Size ◽  
Wild Species ◽  
Flow Cytometric

Nuclear genome size variation in fleshy-fruited Neotropical Myrtaceae

2008 ◽  
Vol 276 (3-4) ◽  
pp. 209-217 ◽  
Author(s):  
Itayguara Ribeiro da Costa ◽  
Marcelo Carnier Dornelas ◽  
Eliana Regina Forni-Martins
Keyword(s):  
Genome Size ◽  
Size Variation ◽  
Nuclear Genome ◽  
Genome Size Variation

scholarly journals Flow Cytometric Determination of Genome Size in the Taxodiaceae, Cupressaceae sensu stricto and Sciadopityaceae.

CYTOLOGIA ◽  
2001 ◽  
Vol 66 (3) ◽  
pp. 307-311 ◽  
Author(s):  
Masahiro Hizume ◽  
Teiji Kondo ◽  
Fukashi Shibata ◽  
Ryoko Ishizuka
Keyword(s):  
Genome Size ◽  
Sensu Stricto ◽  
Flow Cytometric

The Genome Size of Some Species of Hyalomma Ticks from Turkish Thrace 

2018 ◽  
Vol 23 (11) ◽  
pp. 2122 ◽  
Author(s):  
Nadim Yılmazer
Keyword(s):  
Genome Size ◽  
X Chromosome ◽  
Tick Species ◽  
Animal Health ◽  
Chromosome Size ◽  
Diploid Genome ◽  
Flow Cytometric ◽  
Determination System ◽  
Sex Determination System ◽  
First Time

Hyalomma scupense, H. excavatum and H. marginatum are globally important tick species, as well as in Turkey, both in terms of human and animal health. The genome sizes of these tick species were determined in this study for the first time. From flow cytometric measurements, diploid genome sizes of female and male H. scupense were found to be 2.13 pg and 1.75 pg, respectively, while H. excavatum were 2.21 pg and 1.94 pg, and H. marginatum were 2.48 pg and 1.98 pg, respectively. Differences in diploid genome size indicate X chromosome size of females and males in these ticks because they have an XX:XO sex determination system. Thus, it was estimated that the X chromosome of H. scupense, H. excavatum, and H. marginatum may be composed of as much as 0.38 pg, 0.27 pg, and 0.50 pg of DNA, respectively. These findings indicate suitability of these three species for genome sequencing due to the relatively small size of their genomes compared with other tick species.

scholarly journals Nuclear genome size and karyotype analysis in Papaver for BAC library construction

2005 ◽  
Vol 21 (2) ◽  
pp. 145-150
Author(s):  
T. K. Kyrylenko ◽  
O. I. Martynenko ◽  
O. G. Alkhimova
Keyword(s):  
Genome Size ◽  
Karyotype Analysis ◽  
Bac Library ◽  
Nuclear Genome ◽  
Library Construction

Genome size and nucleotypic variation in Malus germplasm

Genome ◽  
2009 ◽  
Vol 52 (2) ◽  
pp. 148-155 ◽  
Author(s):  
Schuyler S. Korban ◽  
Wannasiri Wannarat ◽  
Charlotte M. Rayburn ◽  
Tatiana C. Tatum ◽  
A. Lane Rayburn
Keyword(s):  
Genome Size ◽  
Flow Cytometric Analysis ◽  
Internal Standard ◽  
The United States ◽  
Mean Value ◽  
Ploidy Levels ◽  
Flow Cytometric ◽  
Malus Species ◽  
Leaf Tissues ◽  
Stomatal Length

The genus Malus has anywhere between 25 and 33 species along with several subspecies. Malus species as well as clones within the same species have varying ploidy levels, as these are more than likely collected from different trees and (or) from different locations. In recent years, large numbers of Malus germplasm accessions have been collected and maintained at the United States National Germplasm Clonal Repository; however, genome sizes of this material have not yet been determined. In this study, leaf tissues from young grafted trees of 100 Malus species and hybrids growing in a nursery at the University of Illinois were collected and immediately used for extracting nuclei. Leaf tissues from apple and maize line W-22, used as an internal standard, were co-chopped and prepared for flow cytometric analysis. Apple nuclei were stained with propidium iodide, an intercalating dye, and a minimum of 8000 nuclei per sample were analyzed. Mean fluorescence of apple nuclei was then determined. A total of four replications per sample was used. Among 100 Malus accessions analyzed, one tetraploid, three triploid, and 96 diploid genotypes were identified. Significant differences in genome size were identified among the three ploidy types observed and also within diploid genotypes. The 2C mean value for tetraploids was 3.13 pg and ranged from 2.27 to 2.41 pg for triploids, whereas 2C values for diploids ranged between 1.44 and 1.72 pg. In addition, leaf impressions of young, fully expanded leaves were collected from young trees of 10 selected genotypes based on their ploidy and flow cytometric analysis and used to measure the nucleotypic parameter stomatal length. Ten stomata were measured per slide, three slides were analyzed per leaf, and three leaves were analyzed per accession. Overall, mean length of stomata ranged between 19.47 μm (diploid) and 27.6 μm (tetraploid), indicating that stomatal length in a tetraploid Malus genotype was 1.4-fold higher than that of a diploid genotype. A positive correlation between genome size and the nucleotypic parameter stomatal length was observed.

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