scholarly journals Evaluation of immune response according to the metastatic status in the regional lymph nodes in patients with gastric carcinoma

2010 ◽  
Vol 24 (6) ◽  
Author(s):  
Nakamura
Keyword(s):  
Immune Response ◽  
Gastric Carcinoma ◽  
Lymph Nodes ◽  
Regional Lymph Nodes

scholarly journals Bovine Immune Response to Vaccination and Infection with Leptospira borgpetersenii Serovar Hardjo

mSphere ◽  
2021 ◽  
Vol 6 (2) ◽  
Author(s):  
Jennifer H. Wilson-Welder ◽  
David P. Alt ◽  
Jarlath E. Nally ◽  
Steven C. Olsen
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Lymph Nodes ◽  
Γδ T Cells ◽  
Regional Lymph Nodes ◽  
Content Type ◽  
Oil Emulsion ◽  
Leptospira Borgpetersenii ◽  
Ifn Γ ◽  
Experimental Challenge

ABSTRACT This study examined the humoral and cellular response of cattle vaccinated with two commercial leptospiral vaccines, Leptavoid and Spirovac, and a novel bacterin vaccine using Seppic Montanide oil emulsion adjuvant. Vaccination was followed by experimental challenge. All vaccinated cattle were protected from colonization of the kidney and shedding of Leptospira in urine, as detected by culture and immunofluorescence assay. Agglutinating antibody titers were detected in vaccinated cattle at 4 weeks following vaccination, with small anamnestic response detected following experimental challenge. Only animals vaccinated with the oil emulsion-adjuvanted bacterin produced significant IgG2 titers following vaccination, and nonvaccinated animals produced serum IgA titers after experimental challenge. CD4+ and γδ T cells from vaccinated cattle proliferated when cultured with antigen ex vivo. Cellular responses included a marked proliferation of γδ T cells immediately following experimental challenge in vaccinated cattle and release of gamma interferon (IFN-γ), interleukin 17a (IL-17a), and IL-12p40 from stimulated cells. Proliferative and cytokine responses were found not just in peripheral mononuclear cells but also in lymphocytes isolated from renal lymph nodes at 10 weeks following experimental challenge. Overall, effects of leptospirosis vaccination and infection were subtle, resulting in only modest activation of CD4+ and γδ T cells. The use of Seppic Montanide oil emulsion adjuvants may shorten the initiation of response to vaccination, which could be useful during outbreaks or in areas where leptospirosis is endemic. IMPORTANCE Leptospirosis is an underdiagnosed, underreported zoonotic disease of which domestic livestock can be carriers. As a reservoir host for Leptospira borgpetersenii serovar Hardjo, cattle may present with reproductive issues, including abortion, birth of weak or infected calves, or failure to breed. Despite years of study and the availability of commercial vaccines, detailed analysis of the bovine immune response to vaccination and Leptospira challenge is lacking. This study evaluated immunologic responses to two efficacious commercial vaccines and a novel bacterin vaccine using an adjuvant chosen for enhanced cellular immune responses. Antigen-specific responsive CD4 and γδ T cells were detected following vaccination and were associated with release of inflammatory cytokines IFN-γ and IL-17a after stimulation. CD4 and γδ cells increased in the first week after infection and, combined with serum antibody, may play a role in clearance of bacteria from the blood and resident tissues. Additionally, these antigen-reactive T cells were found in the regional lymph nodes following infection, indicating that memory responses may not be circulating but are still present in regional lymph nodes. The information gained in this study expands knowledge of bovine immune response to leptospirosis vaccines and infection. The use of oil emulsion adjuvants may enhance early immune responses to leptospiral bacterins, which could be useful in outbreaks or situations where leptospirosis is endemic.

Gastric carcinoma with lymphoid stroma: Correlation to reactive hyperplasia in regional lymph nodes and prognosis

1983 ◽  
Vol 13 (3) ◽  
pp. 177-183 ◽  
Author(s):  
Takeshi Okamura ◽  
Yoshifumi Kodama ◽  
Takahisa Kamegawa ◽  
Chiaki Sano ◽  
Ryunosuke Kumashiro ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Lymph Nodes ◽  
Regional Lymph Nodes ◽  
Lymphoid Stroma ◽  
Reactive Hyperplasia

Correlation of peripheral blood lymphocyte counts with outcome and histopathologic features of lymphocytic inflammation in patients with stage II colorectal carcinoma.

2014 ◽  
Vol 32 (3_suppl) ◽  
pp. 475-475
Author(s):  
Hector Hugo Li-Chang ◽  
Naziheh Assarzadegan ◽  
David Messenger ◽  
Andrea Grin ◽  
Christopher Howlett ◽  
...  
Keyword(s):  
Immune Response ◽  
Lymph Node ◽  
Colorectal Carcinoma ◽  
Lymph Nodes ◽  
Tumor Infiltrating Lymphocytes ◽  
Peripheral Lymphocyte ◽  
Stage Ii ◽  
Regional Lymph Nodes ◽  
Reactive Changes ◽  
Infiltrating Lymphocytes

475 Background: The prognostic importance of the local antitumor immune response in colorectal carcinoma (CRC) is well established. Recent studies suggest that systemic antitumor immunity may also impact on survival. This study aimed to determine the relationship between peripheral blood lymphocyte counts (PBLCs), reactive features in regional lymph nodes, local tumoral/peri-tumoral lymphocytic inflammation and survival in stage II CRC. Methods: Resection specimens from 185 patients with stage II CRC were assessed for peri-tumoral lymphocytic inflammation, tumor-infiltrating lymphocytes, lymph node yield, lymph node size, and reactive changes in individual lymph nodes. Reactive changes were scored semi-quantitatively and given a reactivity [R] score of 1-4 based on the presence and size of germinal centers. Pre-operative PBLCs and neutrophil counts were recorded. Results: Lower peripheral lymphocyte counts were associated with advanced age (p = 0.016), T4 disease (p = 0.006), venous invasion (p = 0.006), lymph node yields <12 (p = 0.024), smaller mean nodal diameters (p = 0.021), absence of tumor-infiltrating lymphocytes (p = 0.002) and fewer highly-reactive lymph nodes (R score 4) per case (p = 0.032). Cox regression analysis revealed that advanced age (Hazard Ratio, 1.03 per year [95% confidence interval, 1.01-1.05]; p = 0.015) and higher peripheral lymphocyte counts (HR, 0.52 per 1x109cells/L [95% CI, 0.31-0.86]; p = 0.011) were independently predictive of disease-free survival. Neutrophil counts were not associated with outcome. A higher neutrophil to lymphocyte ratio was associated with worse survival on univariate, but not multivariable analysis. Conclusions: The PBLC is an independent predictor of survival in stage II CRC, and is significantly associated with the immune status of regional lymph nodes and the local lymphocytic immune response.

High Fas expression in gastric carcinoma cells as a factor correlating with the occurrence of metastases to regional lymph nodes

2014 ◽  
Vol 59 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Mariusz Gryko ◽  
Katarzyna Guzińska-Ustymowicz ◽  
Joanna Kiśluk ◽  
Dariusz Cepowicz ◽  
Andrzej Kemona ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Lymph Nodes ◽  
Carcinoma Cells ◽  
Regional Lymph Nodes

scholarly journals Morphometric analysis of regional lymph nodes with and without metastasis from early gastric carcinoma

Cancer ◽  
2000 ◽  
Vol 88 (11) ◽  
pp. 2438-2442 ◽  
Author(s):  
Kenjiro Nakamura ◽  
Takashi Morisaki ◽  
Hirokazu Noshiro ◽  
Nobuhiro Torata ◽  
Naoko Kinukawa ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Lymph Nodes ◽  
Morphometric Analysis ◽  
Early Gastric Carcinoma ◽  
Regional Lymph Nodes
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