Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro

2001 ◽  
Author(s):  
Ken Yoshida ◽  
Hideya Yamazaki ◽  
Shuji Ozeki ◽  
Takehiro Inoue ◽  
Yasuo Yoshioka ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Micronucleus Formation ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Radiation Induced

scholarly journals Anticancer effect of thalidomide in vitro on human osteosarcoma cells

2016 ◽  
Vol 36 (6) ◽  
pp. 3545-3551 ◽  
Author(s):  
Jianwei Zhu ◽  
Ya Yang ◽  
Sihong Liu ◽  
Huihua Xu ◽  
Yong Wu ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Anticancer Effect ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro

2012 ◽  
Vol 12 (1) ◽  
pp. 1183-1188 ◽  
Author(s):  
XING LIU ◽  
WEI LI ◽  
SHUO GENG ◽  
QING-GANG MENG ◽  
ZHENG-GANG BI
Keyword(s):  
Osteosarcoma Cells ◽  
Sonodynamic Therapy ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals Licochalcone A-Induced Apoptosis Through the Activation of p38MAPK Pathway Mediated Mitochondrial Pathways of Apoptosis in Human Osteosarcoma Cells In Vitro and In Vivo

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1441 ◽  
Author(s):  
Lin ◽  
Yang ◽  
Chiou ◽  
Hsieh ◽  
Wen ◽  
...  
Keyword(s):  
Cell Viability ◽  
Mitochondrial Membrane ◽  
Annexin V ◽  
Osteosarcoma Cells ◽  
Antitumor Effects ◽  
Licochalcone A ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

Background: Licochalcone A (LicA) is isolated from the roots of Glycyrrhiza glabra and possesses antitumor and anti-invasive activities against several tumor cells. However, the antitumor effects of LicA on human osteosarcoma cells have yet to be demonstrated either in vitro or in vivo. Methods: Cell viability was measured by MTT assay. Apoptosis and mitochondrial dysfunction were detected with Annexin V/PI staining and JC-1 staining by flow cytometry. The expressions of caspase- or mitochondrial-related proteins were demonstrated by western blotting. Antitumor effect of LicA on 143B xenograft mice in vivo. Results: LicA could inhibit cell proliferation and induce apoptosis in human osteosarcoma cells, as evidenced by a decrease in cell viability, loss of mitochondrial membrane potentials, and activation of caspases. LicA treatment substantially reduced the expression of Bcl-2 and Mcl-1 and increased the expression of cleaved-caspase-3, cleaved-caspase-9, cleaved-PARP, and Bax in HOS and U2OS cells. Moreover, mitochondrial membrane potential and apoptosis suppression mediated by Z-VAD or tauroursodeoxycholic acid significantly reduced LicA-induced mitochondria-dependent apoptosis. The study also determined that LicA treatment induced p38MAPK phosphorylation, but siRNA-p38 or BIRB796 substantially reversed cell viability through the inhibition of mitochondria-dependent apoptosis pathways. Finally, an in vivo study revealed that LicA significantly inhibited 143B xenograft tumor growth. Conclusions: These findings demonstrate that LicA has antitumor activities against human osteosarcoma cells through p38MAPK regulation of mitochondria-mediated intrinsic apoptotic pathways in vitro and in vivo.

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