scholarly journals Anticancer effect of thalidomide in vitro on human osteosarcoma cells

2016 ◽  
Vol 36 (6) ◽  
pp. 3545-3551 ◽  
Author(s):  
Jianwei Zhu ◽  
Ya Yang ◽  
Sihong Liu ◽  
Huihua Xu ◽  
Yong Wu ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Anticancer Effect ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals Bioinspired-Metalloporphyrin Magnetic Nanocomposite as a Reusable Catalyst for Synthesis of Diastereomeric (−)-Isopulegol Epoxide: Anticancer Activity Against Human Osteosarcoma Cells (MG-63)

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 52 ◽  
Author(s):  
Lucas Dias ◽  
Ana Batista de Carvalho ◽  
Sara Pinto ◽  
Gilberto Aquino ◽  
Mário Calvete ◽  
...  
Keyword(s):  
Reusable Catalyst ◽  
Osteosarcoma Cell ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma Cell Line ◽  
Anticancer Effect ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Human Osteosarcoma Cell ◽  
Ic50 Values

In the present study, we developed a green epoxidation approach for the synthesis of the diastereomers of (−)-isopulegol benzyl ether epoxide using molecular oxygen as the oxidant and a hybrid manganese(III)-porphyrin magnetic reusable nanocomposite as the catalyst. High activity, selectivity, and stability were obtained, with up to four recycling cycles without the loss of activity and selectivity for epoxide. The anticancer effect of the newly synthesized isopulegol epoxide diastereomers was evaluated on a human osteosarcoma cell line (MG-63); both diastereomers showed similar in vitro potency. The measured IC50 values were significantly lower than those reported for other monoterpene analogues, rendering these epoxide isomers as promising anti-tumor agents against low prognosis osteosarcoma.

Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro

2001 ◽  
Author(s):  
Ken Yoshida ◽  
Hideya Yamazaki ◽  
Shuji Ozeki ◽  
Takehiro Inoue ◽  
Yasuo Yoshioka ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Micronucleus Formation ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Radiation Induced

scholarly journals Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro

2012 ◽  
Vol 12 (1) ◽  
pp. 1183-1188 ◽  
Author(s):  
XING LIU ◽  
WEI LI ◽  
SHUO GENG ◽  
QING-GANG MENG ◽  
ZHENG-GANG BI
Keyword(s):  
Osteosarcoma Cells ◽  
Sonodynamic Therapy ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals Licochalcone A-Induced Apoptosis Through the Activation of p38MAPK Pathway Mediated Mitochondrial Pathways of Apoptosis in Human Osteosarcoma Cells In Vitro and In Vivo

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1441 ◽  
Author(s):  
Lin ◽  
Yang ◽  
Chiou ◽  
Hsieh ◽  
Wen ◽  
...  
Keyword(s):  
Cell Viability ◽  
Mitochondrial Membrane ◽  
Annexin V ◽  
Osteosarcoma Cells ◽  
Antitumor Effects ◽  
Licochalcone A ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

Background: Licochalcone A (LicA) is isolated from the roots of Glycyrrhiza glabra and possesses antitumor and anti-invasive activities against several tumor cells. However, the antitumor effects of LicA on human osteosarcoma cells have yet to be demonstrated either in vitro or in vivo. Methods: Cell viability was measured by MTT assay. Apoptosis and mitochondrial dysfunction were detected with Annexin V/PI staining and JC-1 staining by flow cytometry. The expressions of caspase- or mitochondrial-related proteins were demonstrated by western blotting. Antitumor effect of LicA on 143B xenograft mice in vivo. Results: LicA could inhibit cell proliferation and induce apoptosis in human osteosarcoma cells, as evidenced by a decrease in cell viability, loss of mitochondrial membrane potentials, and activation of caspases. LicA treatment substantially reduced the expression of Bcl-2 and Mcl-1 and increased the expression of cleaved-caspase-3, cleaved-caspase-9, cleaved-PARP, and Bax in HOS and U2OS cells. Moreover, mitochondrial membrane potential and apoptosis suppression mediated by Z-VAD or tauroursodeoxycholic acid significantly reduced LicA-induced mitochondria-dependent apoptosis. The study also determined that LicA treatment induced p38MAPK phosphorylation, but siRNA-p38 or BIRB796 substantially reversed cell viability through the inhibition of mitochondria-dependent apoptosis pathways. Finally, an in vivo study revealed that LicA significantly inhibited 143B xenograft tumor growth. Conclusions: These findings demonstrate that LicA has antitumor activities against human osteosarcoma cells through p38MAPK regulation of mitochondria-mediated intrinsic apoptotic pathways in vitro and in vivo.

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