scholarly journals Endogenous Leu332Gln mutation in p53 disrupts the tetramerization ability in a canine mammary gland tumor cell line

2018 ◽  
Author(s):  
Kazuhiko Ochiai ◽  
Daigo Azakami ◽  
Masami Morimatsu ◽  
Hinako Hirama ◽  
Shota Kawakami ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cell Line ◽  
Mammary Gland Tumor ◽  
Gland Tumor ◽  
Canine Mammary Gland

scholarly journals Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression

2016 ◽  
Vol 17 (3) ◽  
pp. 385 ◽  
Author(s):  
Tomohiro Osaki ◽  
Yuji Sunden ◽  
Akihiko Sugiyama ◽  
Kazuo Azuma ◽  
Yusuke Murahata ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Tumor Cell ◽  
Cell Line ◽  
Mirna Expression ◽  
Tumor Cell Line ◽  
Mammary Gland Tumor ◽  
Gland Tumor ◽  
Canine Mammary Gland

Effectiveness of small interfering RNA (siRNA) against the Mcl-1 gene in a canine mammary gland tumor cell line

2009 ◽  
Vol 87 (1) ◽  
pp. 64-66 ◽  
Author(s):  
Rui Kano ◽  
Takayuki Yano ◽  
Kota Nagamatsu ◽  
Haruhiko Maruyama ◽  
Hiroshi Kamata ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Tumor Cell ◽  
Cell Line ◽  
Small Interfering Rna ◽  
Tumor Cell Line ◽  
Mammary Gland Tumor ◽  
Gland Tumor ◽  
Interfering Rna ◽  
Canine Mammary Gland

Antitumor Activity of Rivoceranib Against Canine Mammary Gland Tumor Cell Lines

2019 ◽  
Vol 39 (10) ◽  
pp. 5483-5494
Author(s):  
JEONG-HA LEE ◽  
QIANG LI ◽  
JU-HYUN AN ◽  
HYUNG-KYU CHAE ◽  
JIN-WOO CHOI ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Antitumor Activity ◽  
Tumor Cell ◽  
Cell Lines ◽  
Tumor Cell Lines ◽  
Mammary Gland Tumor ◽  
Gland Tumor ◽  
Canine Mammary Gland

scholarly journals Sox4 Regulates the Sensitivity of Canine Mammary Gland Tumor Cells to Cisplatin via the Wnt/β-catenin Signaling Pathway

2021 ◽  
Author(s):  
Mengxin Hu ◽  
Siqi Huang ◽  
Enshuang Xu ◽  
Danning Tong ◽  
Shengzi Jin ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Cell Line ◽  
Signaling Pathway ◽  
Cisplatin Resistance ◽  
Epithelial Mesenchymal Transition ◽  
Mammary Gland Tumor ◽  
Gland Tumor ◽  
Mesenchymal Transition ◽  
Expression Levels ◽  
Canine Mammary Gland

Abstract Background: The development of cisplatin resistance is one of the major causes of breast cancer treatment failure, and is associated with changes in Sox4 gene expression. In this study, a cisplatin-resistant cell line, CHMpCIS, was constructed from the cell line CHMp, which was isolated from the primary lesion of a malignant canine mammary gland tumor (CMGT). Sox4 expression was evaluated to assess its roles in cisplatin sensitivity, proliferation and apoptosis, epithelial-mesenchymal transition (EMT), cancer stem cell (CSC) features, and activation of the Wnt/β-catenin signaling pathway in CMGT cells.Results: CHMpCIS Cells exhibited changes in morphology, slower proliferation, and greater anti-apoptotic ability, EMT and CSC features, and the Wnt/β-catenin pathway was activated in CHMpCIS cells. In CMGT tissues, Sox4 expression was elevated. In CHMpCIS cells, silencing Sox4 inhibited cisplatin resistance, EMT and CSC features, and Wnt/β-catenin signaling activation. Then activating the Wnt/β-catenin signaling pathway increased Sox4 expression levels.Conclusions: Silencing Sox4 inhibited the above-mentioned cancer cell characteristics in CHMpCIS cells compared with CHMp cells. In addition, activating the Wnt/β-catenin signaling pathway increased Sox4 expression levels, as part of a positive feedback loop. These findings may provide new targets and therapeutic strategies for the clinical treatment of CMGT as well as a reference for human mammary gland tumor (HMGT) research.

scholarly journals LncRNA-42060 Regulates Tamoxifen Sensitivity and Tumor Development via Regulating the miR-204-5p/SOX4 Axis in Canine Mammary Gland Tumor Cells

2021 ◽  
Vol 8 ◽  
Author(s):  
Enshuang Xu ◽  
Mengxin Hu ◽  
Reidong Ge ◽  
Danning Tong ◽  
Yuying Fan ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mammary Gland ◽  
Tumor Cells ◽  
Luciferase Reporter ◽  
Drug Resistant ◽  
Mammary Gland Tumor ◽  
Luciferase Reporter Gene ◽  
Gland Tumor ◽  
Canine Mammary Gland ◽  
Resistant Cells

Tamoxifen is the drug of choice for endocrine therapy of breast cancer. Its clinical use is limited by the development of drug resistance. There is increasing evidence that long non-coding RNAs (lncRNAs) are associated with tumor drug resistance. Therefore, we established two TAM-resistant cell lines, CHMpTAM and CHMmTAM. The different expression levels of lncRNA and miRNA in CHMmTAM and CHMm were screened by RNA sequencing, and the lncRNA-miRNA interactions were analyzed. LncRNA ENSCAFG42060 (lnc-42060) was found to be significantly upregulated in drug-resistant cells and tumor tissues. Further functional validation revealed that the knockdown of lnc-42060 inhibited proliferation, migration, clone formation, restoration of TAM sensitivity, and reduction of stem cell formation in drug-resistant cells, whereas overexpression of lnc-4206 showed opposite results. Bioinformatics and dual-luciferase reporter gene assays confirmed that lnc-42060 could act as a sponge for miR-204-5p, further regulating SOX4 expression activity and thus influencing tumor cell progression. In conclusion, we screened lncRNAs and miRNAs associated with TAM resistance in canine mammary gland tumor cells for the first time. lnc-42060 served as a novel marker that may be used as an important biomarker for future diagnosis and treatment.

scholarly journals Adhesional Function of Canine Mammary Gland Tumor Cells Expressing Sialyl Lewis X

2009 ◽  
Vol 71 (9) ◽  
pp. 1225-1228 ◽  
Author(s):  
Takayuki NAKAGAWA ◽  
Yoshifumi ENDO ◽  
Manabu WATANABE ◽  
Manabu MOCHIZUKI ◽  
Ryohei NISHIMURA ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Tumor Cells ◽  
Sialyl Lewis X ◽  
Mammary Gland Tumor ◽  
Gland Tumor ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Canine Mammary Gland

scholarly journals Significance of Tumor-Infiltrating Immune Cells in Spontaneous Canine Mammary Gland Tumor: 140 Cases

2012 ◽  
Vol 74 (2) ◽  
pp. 227-230 ◽  
Author(s):  
Kohei SAEKI ◽  
Yoshifumi ENDO ◽  
Kazuyuki UCHIDA ◽  
Ryohei NISHIMURA ◽  
Nobuo SASAKI ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Immune Cells ◽  
Mammary Gland Tumor ◽  
Gland Tumor ◽  
Canine Mammary Gland
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