scholarly journals Ursodeoxycholic acid suppresses epithelial-mesenchymal transition and cancer stem cell formation by reducing the levels of peroxiredoxin II and reactive oxygen species in pancreatic cancer cells

2017 ◽  
Author(s):  
Yoon Kim ◽  
Seok Jeong ◽  
Eun-Kyung Kim ◽  
Eui Kim ◽  
Jae Cho
Keyword(s):  
Reactive Oxygen Species ◽  
Pancreatic Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Cancer Cells ◽  
Epithelial Mesenchymal Transition ◽  
Cell Formation ◽  
Oxygen Species ◽  
Mesenchymal Transition ◽  
Pancreatic Cancer Cells

scholarly journals Monitoring Epithelial–Mesenchymal Transition of Pancreatic Cancer Cells via Investigation of Mitochondrial Dysfunction

2020 ◽  
Vol 3 (2) ◽  
pp. 32 ◽  
Author(s):  
Jae Jun Sim ◽  
Keun-Yeong Jeong
Keyword(s):  
Pancreatic Cancer ◽  
Membrane Potential ◽  
Mitochondrial Dysfunction ◽  
Cancer Cells ◽  
Epithelial Mesenchymal Transition ◽  
Ros Generation ◽  
Oxygen Species ◽  
Mesenchymal Transition ◽  
Pancreatic Cancer Cells ◽  
Hypoxic Environment

In this protocol, we introduced a method of measuring mitochondrial dysfunction to confirm the epithelial–mesenchymal transition (EMT) in pancreatic cancer cells under a hypoxic environment. There are many expertized and complicated methods to verify EMT. However, our methods have indicated that EMT can be identified by examining changes in reactive oxygen species (ROS) generation and membrane potential in mitochondria. To demonstrate whether the changes in the indicators of mitochondrial dysfunction are correlative to EMT, cell morphology, and expression of E-cadherin and N-cadherin were additionally observed. The results verified that a decrease in membrane potential and an increase in ROS in mitochondria were associated with EMT of pancreatic cancer cells. This protocol would be useful as a basis for providing an additional indicator for changes in the tumor microenvironment of pancreatic cancer cells relating to EMT under a hypoxic environment.

scholarly journals A reactive oxygen species-generating, cyclooxygenase-2 inhibiting, cancer stem cell-potent tetranuclear copper(ii) cluster

2017 ◽  
Vol 46 (38) ◽  
pp. 12785-12789 ◽  
Author(s):  
C. Lu ◽  
K. Laws ◽  
A. Eskandari ◽  
K. Suntharalingam
Keyword(s):  
Reactive Oxygen Species ◽  
Stem Cells ◽  
Schiff Base ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Cancer Stem Cell ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
Cyclooxygenase 2 ◽  
Oxygen Species

Tetranuclear copper(ii) complexes containing multiple diclofenac and Schiff base moieties,1–4, are shown to kill bulk cancer cells and cancer stem cells (CSCs) with low micromolar potency.

scholarly journals Tephrosin induces apoptosis of human pancreatic cancer cells through the generation of reactive oxygen species

2021 ◽  
Vol 12 (1) ◽  
pp. 270-280
Author(s):  
Jie Du ◽  
Fan Jiang ◽  
Shen-Sheng Xu ◽  
Zi-Feng Huang ◽  
Li‐Li Chen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
Human Pancreatic Cancer ◽  
Oxygen Species ◽  
Pancreatic Cancer Cells
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