scholarly journals PEITC inhibits human brain glioblastoma GBM 8401 cell migration and invasion through the inhibition of uPA, Rho A, and Ras with inhibition of MMP-2, -7 and -9 gene expression

2015 ◽  
Vol 34 (5) ◽  
pp. 2489-2496 ◽  
Author(s):  
YU-CHENG CHOU ◽  
MENG-YA CHANG ◽  
MEI-JEN WANG ◽  
FU-SHUN YU ◽  
HSIN-CHUNG LIU ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Migration ◽  
Human Brain ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
Rho A

scholarly journals Galectin-14 Promotes Trophoblast Migration and Invasion by Upregulating the Expression of MMP-9 and N-Cadherin

2021 ◽  
Vol 9 ◽  
Author(s):  
Miaomiao Wang ◽  
Yuqing Xu ◽  
Peng Wang ◽  
Yanfei Xu ◽  
Pengzhen Jin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Migration ◽  
Early Pregnancy ◽  
Pregnancy Loss ◽  
Migration And Invasion ◽  
Early Pregnancy Loss ◽  
Cell Migration And Invasion ◽  
Akt Phosphorylation ◽  
Trophoblast Migration ◽  
Expression And Activity

Galectin-14 is specifically expressed in placental trophoblasts, and its expression is reduced in trophoblasts retrieved from the cervix of women destined to develop early pregnancy loss. However, the roles of galectin-14 in regulating trophoblasts and in the pathogenesis of pregnancy complication have never been investigated. In the current research, we aimed to investigate the roles of galectin-14 in the regulation of trophoblasts. Tissues of the placenta and villi were collected. Primary trophoblasts and human trophoblast cell line HTR-8/SVneo were used. Western blotting and RT-PCR were used to quantify gene expression. The siRNA-mediated galectin-14 knockdown and lentivirus-mediated overexpression were performed to manipulate the gene expression in trophoblasts. Transwell migration and invasion assays were used to evaluate cell migration and invasion capacity. Gelatin zymography was used to determine the gelatinase activity. Galectin-14 was significantly decreased in the villi of early pregnancy loss and the placenta of preeclampsia. Knockdown of galectin-14 in primary trophoblasts inhibited cell migration and invasion, downregulated the expression of matrix metalloproteinase (MMP)-9 and N-cadherin, the activity of MMP-9, and decreased the phosphorylation of Akt. Meanwhile, the overexpression of galectin-14 in HTR-8/SVneo promoted cell migration and invasion, upregulated the expression of MMP-9 and N-cadherin, the activity of MMP-9, and increased the phosphorylation of Akt. Increased Akt phosphorylation promoted cell migration and invasion and upregulated the expression and activity of MMP-9, while decreased Akt phosphorylation inhibited cell migration and invasion and downregulated the expression and activity of MMP-9. Thus, galectin-14 promotes trophoblast migration and invasion by enhancing the expression of MMP-9 and N-cadherin through Akt phosphorylation. The dysregulation of galectin-14 is involved in the pathogenesis of early pregnancy loss and preeclampsia.

Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

2017 ◽  
Vol 32 (11) ◽  
pp. 2400-2413 ◽  
Author(s):  
Yung-Luen Shih ◽  
Man-Kuan Au ◽  
Ko-Lin Liu ◽  
Ming-Yang Yeh ◽  
Ching-Hsiao Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Migration ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
Human Osteosarcoma

miR-20a-enhanced cell migration and invasion via ATg5 in osteosarcoma

2020 ◽  
Vol 44 (4) ◽  
Author(s):  
Huaming Wang ◽  
Jie Yin ◽  
Jin Huang ◽  
Zongwei Liu ◽  
Shengtai Pei
Keyword(s):  
Cell Migration ◽  
Migration And Invasion ◽  
Cell Migration And Invasion
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