Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

2017 ◽  
Vol 32 (11) ◽  
pp. 2400-2413 ◽  
Author(s):  
Yung-Luen Shih ◽  
Man-Kuan Au ◽  
Ko-Lin Liu ◽  
Ming-Yang Yeh ◽  
Ching-Hsiao Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Migration ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
Human Osteosarcoma

scholarly journals Galectin-14 Promotes Trophoblast Migration and Invasion by Upregulating the Expression of MMP-9 and N-Cadherin

2021 ◽  
Vol 9 ◽  
Author(s):  
Miaomiao Wang ◽  
Yuqing Xu ◽  
Peng Wang ◽  
Yanfei Xu ◽  
Pengzhen Jin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Migration ◽  
Early Pregnancy ◽  
Pregnancy Loss ◽  
Migration And Invasion ◽  
Early Pregnancy Loss ◽  
Cell Migration And Invasion ◽  
Akt Phosphorylation ◽  
Trophoblast Migration ◽  
Expression And Activity

Galectin-14 is specifically expressed in placental trophoblasts, and its expression is reduced in trophoblasts retrieved from the cervix of women destined to develop early pregnancy loss. However, the roles of galectin-14 in regulating trophoblasts and in the pathogenesis of pregnancy complication have never been investigated. In the current research, we aimed to investigate the roles of galectin-14 in the regulation of trophoblasts. Tissues of the placenta and villi were collected. Primary trophoblasts and human trophoblast cell line HTR-8/SVneo were used. Western blotting and RT-PCR were used to quantify gene expression. The siRNA-mediated galectin-14 knockdown and lentivirus-mediated overexpression were performed to manipulate the gene expression in trophoblasts. Transwell migration and invasion assays were used to evaluate cell migration and invasion capacity. Gelatin zymography was used to determine the gelatinase activity. Galectin-14 was significantly decreased in the villi of early pregnancy loss and the placenta of preeclampsia. Knockdown of galectin-14 in primary trophoblasts inhibited cell migration and invasion, downregulated the expression of matrix metalloproteinase (MMP)-9 and N-cadherin, the activity of MMP-9, and decreased the phosphorylation of Akt. Meanwhile, the overexpression of galectin-14 in HTR-8/SVneo promoted cell migration and invasion, upregulated the expression of MMP-9 and N-cadherin, the activity of MMP-9, and increased the phosphorylation of Akt. Increased Akt phosphorylation promoted cell migration and invasion and upregulated the expression and activity of MMP-9, while decreased Akt phosphorylation inhibited cell migration and invasion and downregulated the expression and activity of MMP-9. Thus, galectin-14 promotes trophoblast migration and invasion by enhancing the expression of MMP-9 and N-cadherin through Akt phosphorylation. The dysregulation of galectin-14 is involved in the pathogenesis of early pregnancy loss and preeclampsia.

scholarly journals Quercetin Inhibits Cell Migration and Invasion in Human Osteosarcoma Cells

2017 ◽  
Vol 43 (2) ◽  
pp. 553-567 ◽  
Author(s):  
Haifeng Lan ◽  
Wei Hong ◽  
Pan Fan ◽  
Dongyang Qian ◽  
Jianwei Zhu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Migration ◽  
Mouse Model ◽  
Migration And Invasion ◽  
Nude Mouse Model ◽  
Cell Migration And Invasion ◽  
Human Osteosarcoma ◽  
Mg63 Cells ◽  
Mrna And Protein Expression

Background/Aims: Osteosarcoma is a malignant tumor associated with high mortality; however, no effective therapies for the disease have been developed. Several studies have focused on elucidating the pathogenesis of osteosarcoma and have aimed to develop novel therapies for the disease. Quercetin is a vital dietary flavonoid that has been shown to have a variety of anticancer effects, as it induces cell cycle arrest, apoptosis, and differentiation and is involved in cell adhesion, metastasis and angiogenesis. Herein, we aimed to investigate the effects of quercetin on osteosarcoma migration and invasion in vitro and in vivo and to explore the molecular mechanisms underlying its effects on osteosarcoma migration and invasion. Methods: Cell viability, cell cycle activity and cell apoptosis were measured using CCK-8 assay and flow cytometry, and cell migration and invasion were evaluated by wound healing and transwell assays, respectively. The mRNA and protein expression levels of several proteins of interest were assessed by real-time quantitative PCR and western blotting, respectively. Moreover, a nude mouse model of human osteosarcoma lung metastasis was established to assess the anti-metastatic effects of quercetin in vivo. Results: We noted no significant differences in cell cycle activity and apoptosis between HOS and MG63 cells and control cells. Treatment with quercetin significantly attenuated cell migration and invasion in HOS and MG63 cells compared with treatment with control medium. Moreover HIF-1α, VEGF, MMP2, and MMP9 mRNA and protein expression levels were significantly downregulated in HOS cells treated with quercetin compared with HOS cells treated with controls. Additionally, treatment with quercetin attenuated metastatic lung tumor formation and growth in the nude mouse model of osteosarcoma compared with treatment with controls. Conclusion: Our findings regarding the inhibitory effects of quercetin on cell migration and invasion suggest that quercetin may have potential as a therapy for human osteosarcoma.

scholarly journals Fistein Suppresses Human Osteosarcoma U-2 OS Cell Migration and Invasion via Affecting FAK, uPA and NF-ĸB Signaling Pathway In Vitro

In Vivo ◽  
2019 ◽  
Vol 33 (3) ◽  
pp. 801-810 ◽  
Author(s):  
JR-KAI CHEN ◽  
SHU-FEN PENG ◽  
KUANG CHI LAI ◽  
HSIN-CHUNG LIU ◽  
YI-PING HUANG ◽  
...  
Keyword(s):  
Cell Migration ◽  
Signaling Pathway ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
Human Osteosarcoma
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