scholarly journals Ginsenoside Rg3 induces DNA damage in human osteosarcoma cells and reduces MNNG-induced DNA damage and apoptosis in normal human cells

2013 ◽  
Vol 31 (2) ◽  
pp. 919-925 ◽  
Author(s):  
YUE-HUI ZHANG ◽  
HAI-DONG LI ◽  
BO LI ◽  
SHENG-DAN JIANG ◽  
LEI-SHENG JIANG
Keyword(s):  
Dna Damage ◽  
Human Cells ◽  
Ginsenoside Rg3 ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Normal Human ◽  
Normal Human Cells

scholarly journals Reduction of X-ray-induced DNA damage in normal human cells treated with the PrC-210 radioprotector

Biology Open ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. bio035113 ◽  
Author(s):  
Michael Brand ◽  
Matthias Sommer ◽  
Frank Jermusek ◽  
William E. Fahl ◽  
Michael Uder
Keyword(s):  
Dna Damage ◽  
Human Cells ◽  
X Ray ◽  
Normal Human ◽  
Normal Human Cells

scholarly journals Significant Suppression of CT Radiation-Induced DNA Damage in Normal Human Cells by the PrC-210 Radioprotector

2018 ◽  
Vol 190 (2) ◽  
pp. 133 ◽  
Author(s):  
Frank Jermusek ◽  
Chelsea Benedict ◽  
Emma Dreischmeier ◽  
Michael Brand ◽  
Michael Uder ◽  
...  
Keyword(s):  
Dna Damage ◽  
Human Cells ◽  
Significant Suppression ◽  
Ct Radiation ◽  
Radiation Induced ◽  
Normal Human ◽  
Normal Human Cells

scholarly journals EML4-ALK induces cellular senescence in mortal normal human cells and promotes anchorage-independent growth in immortalized normal human cells

2020 ◽  
Author(s):  
Akihiko Miyanaga ◽  
Masaru Matsumoto ◽  
Jessica A. Beck ◽  
Izumi Horikawa ◽  
Takahiro Oike ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cellular Senescence ◽  
Human Fibroblasts ◽  
Human Cells ◽  
Functional Study ◽  
Sequencing Analysis ◽  
Anchorage Independent Growth ◽  
Normal Human ◽  
Normal Human Cells

Abstract Background: Chromosomal inversions involving anaplastic lymphoma kinase (ALK) and echinoderm microtubule associated protein like 4 (EML4) generate a fusion protein EML4-ALK in non-small cell lung cancer (NSCLC). The understanding of EML4-ALK function can be improved by a functional study using normal human cells.Methods: Here we for the first time conduct such study to examine the effects of EML4-ALK on cell proliferation, cellular senescence, DNA damage, gene expression profiles and transformed phenotypes.Results: The lentiviral expression of EML4-ALK in mortal, normal human fibroblasts caused, through its constitutive ALK kinase activity, an early induction of cellular senescence with senescence-associated b-galactosidase activity, upregulation of p16INK4A and p21WAF1, and accumulated DNA damage. In contrast, when EML4-ALK was expressed in normal human fibroblasts immortalized by telomerase reverse transcriptase (hTERT), which is activated in the vast majority of NSCLC, the cells showed accelerated proliferation and acquired anchorage-independent growth ability in soft-agar medium, without accumulated DNA damage, chromosome aberration, nor p53 mutation. EML4-ALK induced the phosphorylation of STAT3 in both mortal and immortalized cells, but RNA sequencing analysis suggested that the different STAT3-regulated signaling pathways contributed to the different phenotypic outcomes in these cells. While EML4-ALK also induced anchorage-independent growth in hTERT-immortalized human bronchial epithelial cells in vitro, the expression of EML4-ALK alone did not cause detectable in vivo tumorigenicity in immunodeficient mice.Conclusions: Our data indicate that the immortalized state is critical for EML4-ALK to manifest its in vitro transforming activity in human cells. This study provides the isogenic pairs of human cells with and without EML4-ALK expression.

scholarly journals Berberine induces apoptosis and DNA damage in MG-63 human osteosarcoma cells

2014 ◽  
Vol 10 (4) ◽  
pp. 1734-1738 ◽  
Author(s):  
YU ZHU ◽  
NAN MA ◽  
HUI-XIANG LI ◽  
LIN TIAN ◽  
YU-FENG BA ◽  
...  
Keyword(s):  
Dna Damage ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals Human RECQL5 participates in the removal of endogenous DNA damage

2012 ◽  
Vol 23 (21) ◽  
pp. 4273-4285 ◽  
Author(s):  
Takashi Tadokoro ◽  
Mahesh Ramamoorthy ◽  
Venkateswarlu Popuri ◽  
Alfred May ◽  
Jingyan Tian ◽  
...  
Keyword(s):  
Dna Damage ◽  
Genome Stability ◽  
Excision Repair ◽  
Human Cells ◽  
Recq Helicase ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Normal Human ◽  
Normal Human Cells ◽  
Base Excision

Human RECQL5 is a member of the RecQ helicase family, which maintains genome stability via participation in many DNA metabolic processes, including DNA repair. Human cells lacking RECQL5 display chromosomal instability. We find that cells depleted of RECQL5 are sensitive to oxidative stress, accumulate endogenous DNA damage, and increase the cellular poly(ADP-ribosyl)ate response. In contrast to the RECQ helicase family members WRN, BLM, and RECQL4, RECQL5 accumulates at laser-induced single-strand breaks in normal human cells. RECQL5 depletion affects the levels of PARP-1 and XRCC1, and our collective results suggest that RECQL5 modulates and/or directly participates in base excision repair of endogenous DNA damage, thereby promoting chromosome stability in normal human cells.

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