Study of the combined effect of X-irradiation and epigallocatechin-gallate (a tea component) on the growth inhibition and induction of apoptosis in human cancer cell lines

2004 ◽  
Author(s):  
Sarah Baatout ◽  
Paul Jacquet ◽  
Hanane Derradji ◽  
Daniella Ooms ◽  
Arlette Michaux ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Epigallocatechin Gallate ◽  
Combined Effect ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
X Irradiation ◽  
Induction Of Apoptosis

Comparative studies of adriamycin and 28-Deacetyl sendanin onin vitro growth inhibition of human cancer cell lines

1994 ◽  
Vol 17 (2) ◽  
pp. 100-103 ◽  
Author(s):  
Hwan Mook Kim ◽  
Goo Taeg Oh ◽  
Sang Bae Han ◽  
Dong Ho Hong ◽  
Bang Yeon Hwang ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Cell Lines ◽  
Cancer Cell ◽  
Comparative Studies ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines

scholarly journals Human cancer cell lines growth inhibition by GTn oligodeoxyribonucleotides recognizing single-stranded DNA-binding proteins

1998 ◽  
Vol 252 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Bruna Scaggiante ◽  
Carla Morassutti ◽  
Barbara Dapas ◽  
Giuseppe Tolazzi ◽  
Franca Ustulin ◽  
...  
Keyword(s):  
Dna Binding ◽  
Growth Inhibition ◽  
Cell Lines ◽  
Cancer Cell ◽  
Binding Proteins ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Dna Binding Proteins ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines

scholarly journals Modulating the activity of short arginine-tryptophan containing antibacterial peptides with N-terminal metallocenoyl groups

2012 ◽  
Vol 8 ◽  
pp. 1753-1764 ◽  
Author(s):  
H Bauke Albada ◽  
Alina-Iulia Chiriac ◽  
Michaela Wenzel ◽  
Maya Penkova ◽  
Julia E Bandow ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Growth Inhibition ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
Inhibition Studies ◽  
Kinetics Of

A series of small synthetic arginine and tryptophan containing peptides was prepared and analyzed for their antibacterial activity. The effect of N-terminal substitution with metallocenoyl groups such as ferrocene (FcCO) and ruthenocene (RcCO) was investigated. Antibacterial activity in different media, growth inhibition, and killing kinetics of the most active peptides were determined. The toxicity of selected derivatives was determined against erythrocytes and three human cancer cell lines. It was shown that the replacement of an N-terminal arginine residue with a metallocenoyl moiety modulates the activity of WRWRW-peptides against Gram-positive and Gram-negative bacteria. MIC values of 2–6 µM for RcCO-W(RW)2 and 1–11 µM for (RW)3 were determined. Interestingly, W(RW)2-peptides derivatized with ferrocene were significantly less active than those derivatized with ruthenocene which have similar structural but different electronic properties, suggesting a major influence of the latter. The high activities observed for the RcCO-W(RW)2- and (RW)3-peptides led to an investigation of the origin of activity of these peptides using several important activity-related parameters. Firstly, killing kinetics of the RcCO-W(RW)2-peptide versus killing kinetics of the (RW)3 derivative showed faster reduction of the colony forming units for the RcCO-W(RW)2-peptide, although MIC values indicated higher activity for the (RW)3-peptide. This was confirmed by growth inhibition studies. Secondly, hemolysis studies revealed that both peptides did not lead to significant destruction of erythrocytes, even up to 500 µg/mL for (RW)3 and 250 µg/mL for RcCO-W(RW)2. In addition, toxicity against three human cancer cell lines (HepG2, HT29, MCF7) showed that the (RW)3-peptide had an IC50 value of ~140 µM and the RcW(RW)2 one of ~90 µM, indicating a potentially interesting therapeutic window. Both the killing kinetics and growth inhibition studies presented in this work point to a membrane-based mode of action for these two peptides, each having different kinetic parameters.

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